Delayed Activation Kinetics of Th2- and Th17 Cells Compared to Th1 Cells

by Andrea Duechting, Anna Przybyla, Stefanie Kuerten, Paul V. Lehmann
Published 2017-09-01

IL-21 in Conjunction with Anti-CD40 and IL-4 Constitutes a Potent Polyclonal B Cell Stimulator for Monitoring Antigen-Specific Memory B Cells

by Fridolin Franke, Greg A. Kirchenbaum, Stefanie Kuerten, Paul V. Lehmann
Published 2020-02-01

Normal Distribution of CD8+ T-Cell-Derived ELISPOT Counts within Replicates Justifies the Reliance on Parametric Statistics for Identifying Positive Responses

by Alexey Y. Karulin, Richard Caspell, Marcus Dittrich, Paul V. Lehmann
Published 2015-03-01

Discordance Between the Predicted Versus the Actually Recognized CD8+ T Cell Epitopes of HCMV pp65 Antigen and Aleatory Epitope Dominance

by Alexander A. Lehmann, Ting Zhang, Pedro A. Reche, Paul V. Lehmann
Published 2021-02-01

An Enhanced ELISPOT Assay for Sensitive Detection of Antigen-Specific T Cell Responses to Borrelia burgdorferi

by Gottfried H. Kellermann, Paul V. Lehmann, Diana R. Roen, Chenggang Jin
Published 2013-09-01

Serial Measurements of Apoptotic Cell Numbers Provide Better Acceptance Criterion for PBMC Quality than a Single Measurement Prior to the T Cell Assay

by Marie Wunsch, Richard Caspell, Stefanie Kuerten, Paul V. Lehmann, Srividya Sundararaman
Published 2015-01-01

Neuroantigen-Specific CD4 Cells Expressing Interferon-γ (IFN-γ), Interleukin (IL)-2 and IL-3 in a Mutually Exclusive Manner Prevail in Experimental Allergic Encephalomyelitis (EAE)...

by Paul V. Lehmann, Maike D. Hesse, Stefan Quast, Alexey Y. Karulin
Published 2012-08-01

How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides?

by Ramu A. Subbramanian, Paul V. Lehmann, Oleg S. Targoni, Ioana Moldovan, Wenji Zhang
Published 2012-10-01

CERI, CEFX, and CPI: Largely Improved Positive Controls for Testing Antigen-Specific T Cell Function in PBMC Compared to CEF

by Alexander A. Lehmann, Pedro A. Reche, Ting Zhang, Maneewan Suwansaard, Paul V. Lehmann
Published 2021-01-01

B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody

by Philipp Fecher, Richard Caspell, Villian Naeem, Alexey Y. Karulin, Stefanie Kuerten, Paul V. Lehmann
Published 2018-05-01

Cytomegalovirus (CMV)-Specific Perforin and Granzyme B ELISPOT Assays Detect Reactivation of CMV Infection in Inflammatory Bowel Disease

by Paul V. Lehmann, Jan Heidemann, Matthias Ross, Dominik Bettenworth, Tobias M. Nowacki, Andreas Lügering
Published 2012-04-01

ELISPOT Assays in 384-Well Format: Up to 30 Data Points with One Million Cells

by Jodi Hanson, Srividya Sundararaman, Richard Caspell, Edith Karacsony, Alexey Y. Karulin, Paul V. Lehmann
Published 2015-01-01

Optimal Thawing of Cryopreserved Peripheral Blood Mononuclear Cells for Use in High-Throughput Human Immune Monitoring Studies

by Ramu A. Subbramanian, Paul V. Lehmann, Richard Caspell, Ioana Moldovan, Jessica Laux, Hari Ramachandran
Published 2012-07-01

Deconvoluting the T Cell Response to SARS-CoV-2: Specificity Versus Chance and Cognate Cross-Reactivity

by Alexander A. Lehmann, Greg A. Kirchenbaum, Ting Zhang, Pedro A. Reche, Paul V. Lehmann
Published 2021-05-01

Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays

by Ramu A. Subbramanian, Wenji Zhang, Edith Karacsony, Mascha S. Recks, Helena Batoulis, Stefanie Kuerten, Paul V. Lehmann
Published 2012-07-01

Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients

by Stefanie Kuerten, Tobias R. Schlingmann, Tarvo Rajasalu, Doychin N. Angelov, Paul V. Lehmann, Magdalena Tary-Lehmann
Published 2008-01-01

A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool

by Annemarie Schiller, Ting Zhang, Ruliang Li, Andrea Duechting, Srividya Sundararaman, Anna Przybyla, Stefanie Kuerten, Paul V. Lehmann
Published 2017-12-01

ELISPOTs Produced by CD8 and CD4 Cells Follow Log Normal Size Distribution Permitting Objective Counting

by Alexey Y. Karulin, Kinga Karacsony, Wenji Zhang, Oleg S. Targoni, Ioana Moldovan, Marcus Dittrich, Srividya Sundararaman, Paul V. Lehmann
Published 2015-01-01

Functional T Cell Reactivity to Melanocyte Antigens Is Lost during the Progression of Malignant Melanoma, but Is Restored by Immunization

by Anna Przybyla, Alexander A. Lehmann, Ting Zhang, Jacek Mackiewicz, Łukasz Galus, Greg A. Kirchenbaum, Andrzej Mackiewicz, Paul V. Lehmann
Published 2021-01-01

Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT

by Magdalena Tary-Lehmann, Paul V. Lehmann, Nicole L. Yonkers, Benigno Rodriguez, Kimberly A. Milkovich, Wenji Zhang, Donald D. Anthony
Published 2012-05-01