Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expression
Abstract Background HOTAIR was known to enhance radioresistance in several cancers. However, the function of HOTAIR on radioresistance involving the regulation of HIF-1α in cervical cancer has not been reported. Methods BALB/c nude mice were injected subcutaneously with HeLa cells and irradiated by...
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doaj-0037db81ceb24ff9887a3fe8f97fbcd92020-11-25T02:01:11ZengBMCRadiation Oncology1748-717X2018-10-011311910.1186/s13014-018-1153-4Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expressionNing Li0Dan-dan Meng1Ling Gao2Yue Xu3Pei-jie Liu4Yong-wei Tian5Zhen-ying Yi6Yan Zhang7Xiao-jing Tie8Zhi-qiao Xu9Tumor Diagnosis and Treatment Center of Kaifeng Central HospitalTumor Diagnosis and Treatment Center of Kaifeng Central HospitalTumor Diagnosis and Treatment Center of Kaifeng Central HospitalTumor Diagnosis and Treatment Center of Kaifeng Central HospitalTumor Diagnosis and Treatment Center of Kaifeng Central HospitalTumor Diagnosis and Treatment Center of Kaifeng Central HospitalTumor Diagnosis and Treatment Center of Kaifeng Central HospitalTumor Diagnosis and Treatment Center of Kaifeng Central HospitalTumor Diagnosis and Treatment Center of Kaifeng Central HospitalTumor Diagnosis and Treatment Center of Kaifeng Central HospitalAbstract Background HOTAIR was known to enhance radioresistance in several cancers. However, the function of HOTAIR on radioresistance involving the regulation of HIF-1α in cervical cancer has not been reported. Methods BALB/c nude mice were injected subcutaneously with HeLa cells and irradiated by X-ray. The tumor volume was measured and the expression of HOTAIR in tumors was detected by quantitative real-time PCR. Western blot was performed to detect the protein level of HIF-1α. MTT (3-(4,5-Dimethylthiazol-2-yl) 22,5-diphenyltetrazolium bromide) assay and the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to examine the cell viability and cell apoptosis of HeLa cells and C33A cells exposed to radiation. Results Radiotherapy inhibited the tumor growth in mice bearing HeLa cells. Radiotherapy reduced the expression of HOTAIR and HIF-1α in tumor tissues and HeLa cells or C33A cells. HOTAIR overexpression abrogated the effect of radiation on the cell viability and cell apoptosis of HeLa and C33A cells. HOTAIR also upregulated the expression of HIF-1α in HeLa and C33A cell exposed to radiation. HIF-1α knockdown reversed increasing cell viability and reducing apoptosis of HeLa and C33A cell induced by HOTAIR overexpression. HOTAIR overexpression promoted tumor growth in mice bearing HeLa and exposed to radiation. Conclusion Radiotherapy might inhibit cervical cancer cell growth through HOTAIR/HIF-1α pathway.http://link.springer.com/article/10.1186/s13014-018-1153-4RadiotherapyCervical cancerHOTAIRHIF-1αCell apoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ning Li Dan-dan Meng Ling Gao Yue Xu Pei-jie Liu Yong-wei Tian Zhen-ying Yi Yan Zhang Xiao-jing Tie Zhi-qiao Xu |
spellingShingle |
Ning Li Dan-dan Meng Ling Gao Yue Xu Pei-jie Liu Yong-wei Tian Zhen-ying Yi Yan Zhang Xiao-jing Tie Zhi-qiao Xu Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expression Radiation Oncology Radiotherapy Cervical cancer HOTAIR HIF-1α Cell apoptosis |
author_facet |
Ning Li Dan-dan Meng Ling Gao Yue Xu Pei-jie Liu Yong-wei Tian Zhen-ying Yi Yan Zhang Xiao-jing Tie Zhi-qiao Xu |
author_sort |
Ning Li |
title |
Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expression |
title_short |
Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expression |
title_full |
Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expression |
title_fullStr |
Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expression |
title_full_unstemmed |
Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expression |
title_sort |
overexpression of hotair leads to radioresistance of human cervical cancer via promoting hif-1α expression |
publisher |
BMC |
series |
Radiation Oncology |
issn |
1748-717X |
publishDate |
2018-10-01 |
description |
Abstract Background HOTAIR was known to enhance radioresistance in several cancers. However, the function of HOTAIR on radioresistance involving the regulation of HIF-1α in cervical cancer has not been reported. Methods BALB/c nude mice were injected subcutaneously with HeLa cells and irradiated by X-ray. The tumor volume was measured and the expression of HOTAIR in tumors was detected by quantitative real-time PCR. Western blot was performed to detect the protein level of HIF-1α. MTT (3-(4,5-Dimethylthiazol-2-yl) 22,5-diphenyltetrazolium bromide) assay and the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to examine the cell viability and cell apoptosis of HeLa cells and C33A cells exposed to radiation. Results Radiotherapy inhibited the tumor growth in mice bearing HeLa cells. Radiotherapy reduced the expression of HOTAIR and HIF-1α in tumor tissues and HeLa cells or C33A cells. HOTAIR overexpression abrogated the effect of radiation on the cell viability and cell apoptosis of HeLa and C33A cells. HOTAIR also upregulated the expression of HIF-1α in HeLa and C33A cell exposed to radiation. HIF-1α knockdown reversed increasing cell viability and reducing apoptosis of HeLa and C33A cell induced by HOTAIR overexpression. HOTAIR overexpression promoted tumor growth in mice bearing HeLa and exposed to radiation. Conclusion Radiotherapy might inhibit cervical cancer cell growth through HOTAIR/HIF-1α pathway. |
topic |
Radiotherapy Cervical cancer HOTAIR HIF-1α Cell apoptosis |
url |
http://link.springer.com/article/10.1186/s13014-018-1153-4 |
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