Transmission of Mycobacterium tuberculosis in a Family Proved by Genotyping
Molecular genetic methods have been applied in various epidemiologic studies including investigations of disease acquisition by contact. This report describes the use of various molecular genetic methods in tracing possible household transmission of tuberculosis by contact. Methods: Four Mycobacteri...
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doaj-01301806083e4733b7bc0e54291df8142020-11-24T20:40:15ZengElsevierJournal of the Formosan Medical Association0929-66462007-10-011061080881410.1016/S0929-6646(08)60045-5Transmission of Mycobacterium tuberculosis in a Family Proved by GenotypingChih-Yun Yang0Ruwen Jou1Pei-Chun Chuang2Jenn-Tyang Chang3Jen-Jyh Lee4Ruay-Ming Huang5Chest Hospital, Department of Health, TaipeiReference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Department of Health, TaipeiReference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Department of Health, TaipeiChest Hospital, Department of Health, TaipeiDivision of Chest Medicine, Department of Internal Medicine, Buddhist Tzu Chi General Hospital and Tzu Chi University, Hualien, TaiwanChest Hospital, Department of Health, TaipeiMolecular genetic methods have been applied in various epidemiologic studies including investigations of disease acquisition by contact. This report describes the use of various molecular genetic methods in tracing possible household transmission of tuberculosis by contact. Methods: Four Mycobacterium tuberculosis strains, each from four members of a family, were first isolated and identified in the clinical laboratory of the Chest Hospital and were submitted to the National Reference Laboratory of Mycobacteriology for further confirmation and genotyping. In this study, IS6110 restriction fragment length polymorphism (RFLP), spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive units—variable number tandem repeats (MIRU—VNTR), and rpoB gene sequencing were used for genotyping. Results: All four strains were found to have identical spoligotypes, MIRU–VNTR patterns, and similar IS6110 RFLP profiles. The results of the drug susceptibility test and of rpoB sequencing showed that all four strains were rifampicin resistant. Conclusion: Household transmission through close contact was thus proved by genotyping. We conclude that all four family members were infected with the same lineage of M. tuberculosis.http://www.sciencedirect.com/science/article/pii/S0929664608600455household transmissiongenotypingMycobacterium tuberculosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chih-Yun Yang Ruwen Jou Pei-Chun Chuang Jenn-Tyang Chang Jen-Jyh Lee Ruay-Ming Huang |
spellingShingle |
Chih-Yun Yang Ruwen Jou Pei-Chun Chuang Jenn-Tyang Chang Jen-Jyh Lee Ruay-Ming Huang Transmission of Mycobacterium tuberculosis in a Family Proved by Genotyping Journal of the Formosan Medical Association household transmission genotyping Mycobacterium tuberculosis |
author_facet |
Chih-Yun Yang Ruwen Jou Pei-Chun Chuang Jenn-Tyang Chang Jen-Jyh Lee Ruay-Ming Huang |
author_sort |
Chih-Yun Yang |
title |
Transmission of Mycobacterium tuberculosis in a Family Proved by Genotyping |
title_short |
Transmission of Mycobacterium tuberculosis in a Family Proved by Genotyping |
title_full |
Transmission of Mycobacterium tuberculosis in a Family Proved by Genotyping |
title_fullStr |
Transmission of Mycobacterium tuberculosis in a Family Proved by Genotyping |
title_full_unstemmed |
Transmission of Mycobacterium tuberculosis in a Family Proved by Genotyping |
title_sort |
transmission of mycobacterium tuberculosis in a family proved by genotyping |
publisher |
Elsevier |
series |
Journal of the Formosan Medical Association |
issn |
0929-6646 |
publishDate |
2007-10-01 |
description |
Molecular genetic methods have been applied in various epidemiologic studies including investigations of disease acquisition by contact. This report describes the use of various molecular genetic methods in tracing possible household transmission of tuberculosis by contact.
Methods: Four Mycobacterium tuberculosis strains, each from four members of a family, were first isolated and identified in the clinical laboratory of the Chest Hospital and were submitted to the National Reference Laboratory of Mycobacteriology for further confirmation and genotyping. In this study, IS6110 restriction fragment length polymorphism (RFLP), spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive units—variable number tandem repeats (MIRU—VNTR), and rpoB gene sequencing were used for genotyping.
Results: All four strains were found to have identical spoligotypes, MIRU–VNTR patterns, and similar IS6110 RFLP profiles. The results of the drug susceptibility test and of rpoB sequencing showed that all four strains were rifampicin resistant.
Conclusion: Household transmission through close contact was thus proved by genotyping. We conclude that all four family members were infected with the same lineage of M. tuberculosis. |
topic |
household transmission genotyping Mycobacterium tuberculosis |
url |
http://www.sciencedirect.com/science/article/pii/S0929664608600455 |
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