<i>Bacillus cytotoxicus</i> Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity
Geothermal areas are the niches of a rich microbial diversity that is not only part of the intangible patrimony of a country but also the source of many microbial species with potential biotechnological applications. Particularly, microbial species in geothermal areas in Argentina have been scarcely...
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doaj-013f0139d331433fbd71d8a8eb80eb1c2020-11-25T02:23:30ZengMDPI AGMicroorganisms2076-26072020-05-01879679610.3390/microorganisms8060796<i>Bacillus cytotoxicus</i> Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic ActivityIvana Cavello0María Sofía Urbieta1Sebastián Cavalitto2Edgardo Donati3CINDEFI (CCT La Plata-CONICET, UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, (B1900ASH) La Plata 1900, ArgentinaCINDEFI (CCT La Plata-CONICET, UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, (B1900ASH) La Plata 1900, ArgentinaCINDEFI (CCT La Plata-CONICET, UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, (B1900ASH) La Plata 1900, ArgentinaCINDEFI (CCT La Plata-CONICET, UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, (B1900ASH) La Plata 1900, ArgentinaGeothermal areas are the niches of a rich microbial diversity that is not only part of the intangible patrimony of a country but also the source of many microbial species with potential biotechnological applications. Particularly, microbial species in geothermal areas in Argentina have been scarcely explored regarding their possible biotechnological uses. The purpose of this work was to explore the proteolytic and keratinolytic enzymatic potential of microorganisms that inhabit in the Domuyo geothermal area in the Neuquén Province. To this end, we did enrichment cultures from two high-temperature natural samples in mineral media only supplemented with whole chicken feathers. After the isolation and the phylogenetic and morphologic characterization of different colonies, we obtained a collection of <i>Bacillus cytotoxicus </i>isolates, a species with no previous report of keratinolytic activity and only reported in rehydrated meals connected with food poisoning outbreaks. Its natural habitat has been unknown up to now. We characterized the proteolytic and keratinolytic capacities of the <i>B.</i> <i>cytotoxicus</i> isolates in different conditions, which proved to be remarkably high compared with those of other similar species. Thus, our work represents the first report of the isolation as well as the keratinolytic capacity characterization of strains of <i>B. cytotixicus</i> obtained from a natural environment.https://www.mdpi.com/2076-2607/8/6/796Domuyo geothermal areakeratinasesfeather wastecytK-1 toxin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ivana Cavello María Sofía Urbieta Sebastián Cavalitto Edgardo Donati |
spellingShingle |
Ivana Cavello María Sofía Urbieta Sebastián Cavalitto Edgardo Donati <i>Bacillus cytotoxicus</i> Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity Microorganisms Domuyo geothermal area keratinases feather waste cytK-1 toxin |
author_facet |
Ivana Cavello María Sofía Urbieta Sebastián Cavalitto Edgardo Donati |
author_sort |
Ivana Cavello |
title |
<i>Bacillus cytotoxicus</i> Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity |
title_short |
<i>Bacillus cytotoxicus</i> Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity |
title_full |
<i>Bacillus cytotoxicus</i> Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity |
title_fullStr |
<i>Bacillus cytotoxicus</i> Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity |
title_full_unstemmed |
<i>Bacillus cytotoxicus</i> Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity |
title_sort |
<i>bacillus cytotoxicus</i> isolated from a pristine natural geothermal area reveals high keratinolytic activity |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-05-01 |
description |
Geothermal areas are the niches of a rich microbial diversity that is not only part of the intangible patrimony of a country but also the source of many microbial species with potential biotechnological applications. Particularly, microbial species in geothermal areas in Argentina have been scarcely explored regarding their possible biotechnological uses. The purpose of this work was to explore the proteolytic and keratinolytic enzymatic potential of microorganisms that inhabit in the Domuyo geothermal area in the Neuquén Province. To this end, we did enrichment cultures from two high-temperature natural samples in mineral media only supplemented with whole chicken feathers. After the isolation and the phylogenetic and morphologic characterization of different colonies, we obtained a collection of <i>Bacillus cytotoxicus </i>isolates, a species with no previous report of keratinolytic activity and only reported in rehydrated meals connected with food poisoning outbreaks. Its natural habitat has been unknown up to now. We characterized the proteolytic and keratinolytic capacities of the <i>B.</i> <i>cytotoxicus</i> isolates in different conditions, which proved to be remarkably high compared with those of other similar species. Thus, our work represents the first report of the isolation as well as the keratinolytic capacity characterization of strains of <i>B. cytotixicus</i> obtained from a natural environment. |
topic |
Domuyo geothermal area keratinases feather waste cytK-1 toxin |
url |
https://www.mdpi.com/2076-2607/8/6/796 |
work_keys_str_mv |
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