| Summary: | <p>Abstract</p> <p>Background</p> <p>LRP5, a member of the low density lipoprotein receptor superfamily, regulates diverse developmental processes in embryogenesis and maintains physiological homeostasis in adult organisms. However, how the expression of human <it>LRP5 </it>gene is regulated remains unclear.</p> <p>Results</p> <p>In order to characterize the transcriptional regulation of human <it>LRP5 </it>gene, we cloned the 5' flanking region and evaluated its transcriptional activity in a luciferase reporter system. We demonstrated that both KLF15 and Sp1 binding sites between -72 bp and -53 bp contribute to the transcriptional activation of human <it>LRP5 </it>promoter. Chromatin immunoprecipitation assay demonstrated that the ubiquitous transcription factors KLF15 and Sp1 bind to this region. Using <it>Drosophila </it>SL2 cells, we showed that KLF15 and Sp1 trans-activated the <it>LRP5 </it>promoter in a manner dependent on the presence of Sp1-binding and KLF15-binding motifs.</p> <p>Conclusions</p> <p>Both KLF15 and Sp1 binding sites contribute to the basal activity of human <it>LRP5 </it>promoter. This study provides the first insight into the mechanisms by which transcription of human <it>LRP5 </it>gene is regulated.</p>
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