Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53

Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53

Summary: Targeted knockin mediated by double-stranded DNA cleavage is accompanied by unwanted insertions and deletions (indels) at on-target and off-target sites. A nick-mediated approach scarcely generates indels but exhibits reduced efficiency of targeted knockin. Here, we demonstrate that tandem...

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Bibliographic Details
Main Authors: Toshinori Hyodo, Md. Lutfur Rahman, Sivasundaram Karnan, Takuji Ito, Atsushi Toyoda, Akinobu Ota, Md Wahiduzzaman, Shinobu Tsuzuki, Yohei Okada, Yoshitaka Hosokawa, Hiroyuki Konishi
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124719317218

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http://www.sciencedirect.com/science/article/pii/S2211124719317218

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