Retinal Ganglion Cell Diversity and Subtype Specification from Human Pluripotent Stem Cells
Summary: Retinal ganglion cells (RGCs) are the projection neurons of the retina and transmit visual information to postsynaptic targets in the brain. While this function is shared among nearly all RGCs, this class of cell is remarkably diverse, comprised of multiple subtypes. Previous efforts have i...
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doaj-05bed16f4f6a49e4a9581f6eda7310392020-11-24T23:59:50ZengElsevierStem Cell Reports2213-67112018-04-0110412821293Retinal Ganglion Cell Diversity and Subtype Specification from Human Pluripotent Stem CellsKirstin B. Langer0Sarah K. Ohlemacher1M. Joseph Phillips2Clarisse M. Fligor3Peng Jiang4David M. Gamm5Jason S. Meyer6Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USADepartment of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USAWaisman Center, University of Wisconsin-Madison, Madison, WI 53705, USA; McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI 53705, USADepartment of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USAMorgridge Institute for Research, Madison, WI 53705, USAWaisman Center, University of Wisconsin-Madison, Madison, WI 53705, USA; McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI 53705, USA; Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI 53705, USADepartment of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USA; Department of Medical and Molecular Genetics, Indiana University, Indianapolis, IN 46202, USA; Stark Neurosciences Research Institute, Indiana University, Indianapolis, IN 46202, USA; Corresponding authorSummary: Retinal ganglion cells (RGCs) are the projection neurons of the retina and transmit visual information to postsynaptic targets in the brain. While this function is shared among nearly all RGCs, this class of cell is remarkably diverse, comprised of multiple subtypes. Previous efforts have identified numerous RGC subtypes in animal models, but less attention has been paid to human RGCs. Thus, efforts of this study examined the diversity of RGCs differentiated from human pluripotent stem cells (hPSCs) and characterized defined subtypes through the expression of subtype-specific markers. Further investigation of these subtypes was achieved using single-cell transcriptomics, confirming the combinatorial expression of molecular markers associated with these subtypes, and also provided insight into more subtype-specific markers. Thus, the results of this study describe the derivation of RGC subtypes from hPSCs and will support the future exploration of phenotypic and functional diversity within human RGCs. : In this article, Langer and colleagues present extensive characterization of RGC subtypes derived from human pluripotent stem cells, with multiple subtypes identified by subtype-specific molecular markers. Their results present a more detailed analysis of RGC diversity in human cells and yield the use of different markers to identify RGC subtypes. Keywords: iPSC, retina, retinal ganglion cell, RGC subtype, stem cell, ipRGC, alpha RGC, direction selective RGC, RNA-seqhttp://www.sciencedirect.com/science/article/pii/S2213671118301012 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kirstin B. Langer Sarah K. Ohlemacher M. Joseph Phillips Clarisse M. Fligor Peng Jiang David M. Gamm Jason S. Meyer |
spellingShingle |
Kirstin B. Langer Sarah K. Ohlemacher M. Joseph Phillips Clarisse M. Fligor Peng Jiang David M. Gamm Jason S. Meyer Retinal Ganglion Cell Diversity and Subtype Specification from Human Pluripotent Stem Cells Stem Cell Reports |
author_facet |
Kirstin B. Langer Sarah K. Ohlemacher M. Joseph Phillips Clarisse M. Fligor Peng Jiang David M. Gamm Jason S. Meyer |
author_sort |
Kirstin B. Langer |
title |
Retinal Ganglion Cell Diversity and Subtype Specification from Human Pluripotent Stem Cells |
title_short |
Retinal Ganglion Cell Diversity and Subtype Specification from Human Pluripotent Stem Cells |
title_full |
Retinal Ganglion Cell Diversity and Subtype Specification from Human Pluripotent Stem Cells |
title_fullStr |
Retinal Ganglion Cell Diversity and Subtype Specification from Human Pluripotent Stem Cells |
title_full_unstemmed |
Retinal Ganglion Cell Diversity and Subtype Specification from Human Pluripotent Stem Cells |
title_sort |
retinal ganglion cell diversity and subtype specification from human pluripotent stem cells |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2018-04-01 |
description |
Summary: Retinal ganglion cells (RGCs) are the projection neurons of the retina and transmit visual information to postsynaptic targets in the brain. While this function is shared among nearly all RGCs, this class of cell is remarkably diverse, comprised of multiple subtypes. Previous efforts have identified numerous RGC subtypes in animal models, but less attention has been paid to human RGCs. Thus, efforts of this study examined the diversity of RGCs differentiated from human pluripotent stem cells (hPSCs) and characterized defined subtypes through the expression of subtype-specific markers. Further investigation of these subtypes was achieved using single-cell transcriptomics, confirming the combinatorial expression of molecular markers associated with these subtypes, and also provided insight into more subtype-specific markers. Thus, the results of this study describe the derivation of RGC subtypes from hPSCs and will support the future exploration of phenotypic and functional diversity within human RGCs. : In this article, Langer and colleagues present extensive characterization of RGC subtypes derived from human pluripotent stem cells, with multiple subtypes identified by subtype-specific molecular markers. Their results present a more detailed analysis of RGC diversity in human cells and yield the use of different markers to identify RGC subtypes. Keywords: iPSC, retina, retinal ganglion cell, RGC subtype, stem cell, ipRGC, alpha RGC, direction selective RGC, RNA-seq |
url |
http://www.sciencedirect.com/science/article/pii/S2213671118301012 |
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