Competitive impedimetric aptasensors for detection of small molecule pollutants by the signal amplification of self-assembled biotin-phenylalanine nanoparticle networks
Impedimetric aptasensors without signal amplification exhibit poor sensitivity for the detection of small molecule contaminants. In this work, biotinylated nanoparticles of biotin-FNPs were readily prepared by the self-assembly of biotin-phenylalanine (biotin-Phe) monomers. The biotin-FNPs were then...
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doaj-0607000aed7b49999ed9ad3c3e96c5fc2020-11-25T03:38:39ZengElsevierElectrochemistry Communications1388-24812020-09-01118106791Competitive impedimetric aptasensors for detection of small molecule pollutants by the signal amplification of self-assembled biotin-phenylalanine nanoparticle networksMing La0Daohong Wu1Yanping Gao2Ning Xia3Yifan Niu4Lin Liu5Xinyao Yi6College of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan, Henan 467000, People’s Republic of China; Henan Province of Key Laboratory of New Optoelectronic Functional Materials, Anyang, Henan 455000, People’s Republic of ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, People’s Republic of ChinaCollege of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan, Henan 467000, People’s Republic of ChinaHenan Province of Key Laboratory of New Optoelectronic Functional Materials, Anyang, Henan 455000, People’s Republic of ChinaHenan Province of Key Laboratory of New Optoelectronic Functional Materials, Anyang, Henan 455000, People’s Republic of ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, People’s Republic of China; Henan Province of Key Laboratory of New Optoelectronic Functional Materials, Anyang, Henan 455000, People’s Republic of ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, People’s Republic of China; Corresponding author.Impedimetric aptasensors without signal amplification exhibit poor sensitivity for the detection of small molecule contaminants. In this work, biotinylated nanoparticles of biotin-FNPs were readily prepared by the self-assembly of biotin-phenylalanine (biotin-Phe) monomers. The biotin-FNPs were then used for the development of competitive impedimetric aptasensors by streptavidin–biotin (SA–biotin) coupling chemistry. Specifically, capture of biotinylated DNA (biotin-DNA) by the aptamer-modified electrode allowed for the in situ formation of SA–biotin-FNPs networks on the electrode surface, hampering the electron transfer by creating an insulating layer. The target–aptamer interaction prevented the capture of biotin-DNA, thus inhibiting the formation of SA–biotin-FNPs networks on the electrode surface and allowing for the electron transfer. To demonstrate the analytical performances of the strategy, aflatoxin B1 (AFB1) was determined as the model analyte. The aptasensor exhibited a linear range of 0.05–3 pg/mL. The detectable concentration is much lower than that achieved by other impedimetric aptasensors. The strategy may provide a general way for the design of biosensors to determine various small molecules by matching sequence-specific aptamers.http://www.sciencedirect.com/science/article/pii/S1388248120301429AptasensorElectrochemical impedance spectroscopySmall moleculesSignal amplificationNanomaterials |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ming La Daohong Wu Yanping Gao Ning Xia Yifan Niu Lin Liu Xinyao Yi |
spellingShingle |
Ming La Daohong Wu Yanping Gao Ning Xia Yifan Niu Lin Liu Xinyao Yi Competitive impedimetric aptasensors for detection of small molecule pollutants by the signal amplification of self-assembled biotin-phenylalanine nanoparticle networks Electrochemistry Communications Aptasensor Electrochemical impedance spectroscopy Small molecules Signal amplification Nanomaterials |
author_facet |
Ming La Daohong Wu Yanping Gao Ning Xia Yifan Niu Lin Liu Xinyao Yi |
author_sort |
Ming La |
title |
Competitive impedimetric aptasensors for detection of small molecule pollutants by the signal amplification of self-assembled biotin-phenylalanine nanoparticle networks |
title_short |
Competitive impedimetric aptasensors for detection of small molecule pollutants by the signal amplification of self-assembled biotin-phenylalanine nanoparticle networks |
title_full |
Competitive impedimetric aptasensors for detection of small molecule pollutants by the signal amplification of self-assembled biotin-phenylalanine nanoparticle networks |
title_fullStr |
Competitive impedimetric aptasensors for detection of small molecule pollutants by the signal amplification of self-assembled biotin-phenylalanine nanoparticle networks |
title_full_unstemmed |
Competitive impedimetric aptasensors for detection of small molecule pollutants by the signal amplification of self-assembled biotin-phenylalanine nanoparticle networks |
title_sort |
competitive impedimetric aptasensors for detection of small molecule pollutants by the signal amplification of self-assembled biotin-phenylalanine nanoparticle networks |
publisher |
Elsevier |
series |
Electrochemistry Communications |
issn |
1388-2481 |
publishDate |
2020-09-01 |
description |
Impedimetric aptasensors without signal amplification exhibit poor sensitivity for the detection of small molecule contaminants. In this work, biotinylated nanoparticles of biotin-FNPs were readily prepared by the self-assembly of biotin-phenylalanine (biotin-Phe) monomers. The biotin-FNPs were then used for the development of competitive impedimetric aptasensors by streptavidin–biotin (SA–biotin) coupling chemistry. Specifically, capture of biotinylated DNA (biotin-DNA) by the aptamer-modified electrode allowed for the in situ formation of SA–biotin-FNPs networks on the electrode surface, hampering the electron transfer by creating an insulating layer. The target–aptamer interaction prevented the capture of biotin-DNA, thus inhibiting the formation of SA–biotin-FNPs networks on the electrode surface and allowing for the electron transfer. To demonstrate the analytical performances of the strategy, aflatoxin B1 (AFB1) was determined as the model analyte. The aptasensor exhibited a linear range of 0.05–3 pg/mL. The detectable concentration is much lower than that achieved by other impedimetric aptasensors. The strategy may provide a general way for the design of biosensors to determine various small molecules by matching sequence-specific aptamers. |
topic |
Aptasensor Electrochemical impedance spectroscopy Small molecules Signal amplification Nanomaterials |
url |
http://www.sciencedirect.com/science/article/pii/S1388248120301429 |
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