Asymbiotic germination <i>in vitro</i> in seeds of four species of Cuban orchids

• Main Authors: Loexis Rodríguez, José R. Valles, Roberto González, Karen Alvarado, Enidia Telles, Amauri Díaz, Esmérida Sánchez
• Format: Article
• Language: English
• Published: Universidad Central Marta Abreu de Las Villas 2001-04-01
• Series: Biotecnología Vegetal
• Online Access: https://revista.ibp.co.cu/index.php/BV/article/view/75

About the most of 300 species of Cuban native orchids is have few reference of the micropropagation in the science literature. However, the biotechnical techniques fulfill an important acting in the rescue of threatened species. By that in the laboratory of Vegetable Biotechnology of the Mountain Development Center in Guantanamo, aspects related with the germination were studied in vitro of Campylocentrum micrantrum, Encyclia cochleata, Epidendrum difforme and Oceoclade maculata using the salts of the culture medium Murashige and Skoog (1962) and Knudson (1946), increasing with activate charcoal (0; 1; 2 g.l-1), Agar tachnical No. 3 6 g.l-1 and the adjusted pH at 5.6. The seeds was incubated under conditions of 16 hours light to intensity of 27 uMol.m².s-¹ and 24 ± 1 °C. The reached results showed that the germination of the studied species was obtained under different times and conditions of the medium of cultivation where it stood out for the smallest time used for the germination Encyclia cochleata to the eight weeks in the salts of Murashige and Skoog (1962) and without the addition of activated charcoal, as long as Oceoclade maculata needed 24 weeks to germinate. Key words: antioxidante, biotechnology, flowers, micropropagación, native species