Cohesin cleavage by separase is enhanced by a substrate motif distinct from the cleavage site
Prior to anaphase, securin binds separase and thereby prevents cohesin cleavage. Here, the authors develop a method to produce active securin-free separase, identify a docking motif in cohesin that promotes cleavage, and show that securin interferes with this interaction.
Main Authors: | Laura E. Rosen, Joseph E. Klebba, Jonathan B. Asfaha, Chloe M. Ghent, Melody G. Campbell, Yifan Cheng, David O. Morgan |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2019-11-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-019-13209-y |
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