Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study

Exiguobacterium is a polyextremophile bacterial genus with a physiology that allows it to develop in different adverse environments. The Salar de Huasco is one of these environments due to its altitude, atmospheric pressure, solar radiation, temperature variations, pH, salinity, and the presence of...

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Main Authors: Juan Castro-Severyn, Coral Pardo-Esté, Yoelvis Sulbaran, Carolina Cabezas, Valentina Gariazzo, Alan Briones, Naiyulin Morales, Martial Séveno, Mathilde Decourcelle, Nicolas Salvetat, Francisco Remonsellez, Eduardo Castro-Nallar, Franck Molina, Laurence Molina, Claudia P. Saavedra
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-09-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.02161/full
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language English
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author Juan Castro-Severyn
Juan Castro-Severyn
Coral Pardo-Esté
Yoelvis Sulbaran
Carolina Cabezas
Valentina Gariazzo
Alan Briones
Naiyulin Morales
Martial Séveno
Mathilde Decourcelle
Nicolas Salvetat
Francisco Remonsellez
Francisco Remonsellez
Eduardo Castro-Nallar
Franck Molina
Laurence Molina
Claudia P. Saavedra
spellingShingle Juan Castro-Severyn
Juan Castro-Severyn
Coral Pardo-Esté
Yoelvis Sulbaran
Carolina Cabezas
Valentina Gariazzo
Alan Briones
Naiyulin Morales
Martial Séveno
Mathilde Decourcelle
Nicolas Salvetat
Francisco Remonsellez
Francisco Remonsellez
Eduardo Castro-Nallar
Franck Molina
Laurence Molina
Claudia P. Saavedra
Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study
Frontiers in Microbiology
Exiguobacterium
arsenic
tolerance
proteomic
polyextremophile
author_facet Juan Castro-Severyn
Juan Castro-Severyn
Coral Pardo-Esté
Yoelvis Sulbaran
Carolina Cabezas
Valentina Gariazzo
Alan Briones
Naiyulin Morales
Martial Séveno
Mathilde Decourcelle
Nicolas Salvetat
Francisco Remonsellez
Francisco Remonsellez
Eduardo Castro-Nallar
Franck Molina
Laurence Molina
Claudia P. Saavedra
author_sort Juan Castro-Severyn
title Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study
title_short Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study
title_full Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study
title_fullStr Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study
title_full_unstemmed Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study
title_sort arsenic response of three altiplanic exiguobacterium strains with different tolerance levels against the metalloid species: a proteomics study
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2019-09-01
description Exiguobacterium is a polyextremophile bacterial genus with a physiology that allows it to develop in different adverse environments. The Salar de Huasco is one of these environments due to its altitude, atmospheric pressure, solar radiation, temperature variations, pH, salinity, and the presence of toxic compounds such as arsenic. However, the physiological and/or molecular mechanisms that enable them to prosper in these environments have not yet been described. Our research group has isolated several strains of Exiguobacterium genus from different sites of Salar de Huasco, which show different resistance levels to As(III) and As(V). In this work, we compare the protein expression patterns of the three strains in response to arsenic by a proteomic approach; strains were grown in absence of the metalloid and in presence of As(III) and As(V) sublethal concentrations and the protein separation was carried out in 2D electrophoresis gels (2D-GE). In total, 999 spots were detected, between 77 and 173 of which showed significant changes for As(III) among the three strains, and between 90 and 143 for As(V), respectively, compared to the corresponding control condition. Twenty-seven of those were identified by mass spectrometry (MS). Among these identified proteins, the ArsA [ATPase from the As(III) efflux pump] was found to be up-regulated in response to both arsenic conditions in the three strains, as well as the Co-enzyme A disulfide reductase (Cdr) in the two more resistant strains. Interestingly, in this genus the gene that codifies for Cdr is found within the genic context of the ars operon. We suggest that this protein could be restoring antioxidants molecules, necessary for the As(V) reduction. Additionally, among the proteins that change their expression against As, we found several with functions relevant to stress response, e.g., Hpf, LuxS, GLpX, GlnE, and Fur. This study allowed us to shed light into the physiology necessary for these bacteria to be able to tolerate the toxicity and stress generated by the presence of arsenic in their niche.
topic Exiguobacterium
arsenic
tolerance
proteomic
polyextremophile
url https://www.frontiersin.org/article/10.3389/fmicb.2019.02161/full
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spelling doaj-0ac5d238306c44cd86ef8cc1d36c8eed2020-11-25T01:22:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-09-011010.3389/fmicb.2019.02161477326Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics StudyJuan Castro-Severyn0Juan Castro-Severyn1Coral Pardo-Esté2Yoelvis Sulbaran3Carolina Cabezas4Valentina Gariazzo5Alan Briones6Naiyulin Morales7Martial Séveno8Mathilde Decourcelle9Nicolas Salvetat10Francisco Remonsellez11Francisco Remonsellez12Eduardo Castro-Nallar13Franck Molina14Laurence Molina15Claudia P. Saavedra16Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileCenter for Bioinformatics and Integrative Biology, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileLaboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileLaboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileLaboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileLaboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileLaboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileLaboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileBioCampus Montpellier, CNRS, INSERM, Université de Montpellier, Montpellier, FranceBioCampus Montpellier, CNRS, INSERM, Université de Montpellier, Montpellier, FranceSys2Diag, UMR9005 CNRS ALCEDIAG, Montpellier, FranceLaboratorio de Microbiología Aplicada y Extremófilos, Departamento de Ingeniería Química, Facultad de Ingeniería y Ciencias Geológicas, Universidad Católica del Norte, Antofagasta, ChileCentro de Investigación Tecnológica del Agua en el Desierto (CEITSAZA), Universidad Católica del Norte, Antofagasta, ChileCenter for Bioinformatics and Integrative Biology, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileSys2Diag, UMR9005 CNRS ALCEDIAG, Montpellier, FranceSys2Diag, UMR9005 CNRS ALCEDIAG, Montpellier, FranceLaboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, ChileExiguobacterium is a polyextremophile bacterial genus with a physiology that allows it to develop in different adverse environments. The Salar de Huasco is one of these environments due to its altitude, atmospheric pressure, solar radiation, temperature variations, pH, salinity, and the presence of toxic compounds such as arsenic. However, the physiological and/or molecular mechanisms that enable them to prosper in these environments have not yet been described. Our research group has isolated several strains of Exiguobacterium genus from different sites of Salar de Huasco, which show different resistance levels to As(III) and As(V). In this work, we compare the protein expression patterns of the three strains in response to arsenic by a proteomic approach; strains were grown in absence of the metalloid and in presence of As(III) and As(V) sublethal concentrations and the protein separation was carried out in 2D electrophoresis gels (2D-GE). In total, 999 spots were detected, between 77 and 173 of which showed significant changes for As(III) among the three strains, and between 90 and 143 for As(V), respectively, compared to the corresponding control condition. Twenty-seven of those were identified by mass spectrometry (MS). Among these identified proteins, the ArsA [ATPase from the As(III) efflux pump] was found to be up-regulated in response to both arsenic conditions in the three strains, as well as the Co-enzyme A disulfide reductase (Cdr) in the two more resistant strains. Interestingly, in this genus the gene that codifies for Cdr is found within the genic context of the ars operon. We suggest that this protein could be restoring antioxidants molecules, necessary for the As(V) reduction. Additionally, among the proteins that change their expression against As, we found several with functions relevant to stress response, e.g., Hpf, LuxS, GLpX, GlnE, and Fur. This study allowed us to shed light into the physiology necessary for these bacteria to be able to tolerate the toxicity and stress generated by the presence of arsenic in their niche.https://www.frontiersin.org/article/10.3389/fmicb.2019.02161/fullExiguobacteriumarsenictoleranceproteomicpolyextremophile