Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. In the p...

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Bibliographic Details
Main Authors: Takuya Sato, Tetsushi Sakuma, Tetsuhiro Yokonishi, Kumiko Katagiri, Satoshi Kamimura, Narumi Ogonuki, Atsuo Ogura, Takashi Yamamoto, Takehiko Ogawa
Format: Article
Language:English
Published: Elsevier 2015-07-01
Series:Stem Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S221367111500154X

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