Establishment of a Micropropagation Protocol for Elite Accessions of Bael (Aegle marmelos (L.) Corr.), a Tropical Hardwood Species

The limited availability of high-quality planting material hinders the popularization of bael (Aegle marmelos (L.) Corr.) as a profitable cash crop in Sri Lanka. Five elite bael accessions, namely, Beheth Beli, Paragammana, Mawanella, Rambukkana, and Polonnaruwa-Supun, have been identified and used...

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Main Authors: Chamila Pathirana, Udayanthi Attanayake, Udula Dissanayake, Lakshman Gamlath, Kalyani Ketipearachchi, Terrence Madhujith, Janakie Eeswara
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:Advances in Agriculture
Online Access:http://dx.doi.org/10.1155/2020/8840386
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spelling doaj-0b9320570642457b937f7ca44e9a1b1e2020-11-25T03:17:33ZengHindawi LimitedAdvances in Agriculture2356-654X2314-75392020-01-01202010.1155/2020/88403868840386Establishment of a Micropropagation Protocol for Elite Accessions of Bael (Aegle marmelos (L.) Corr.), a Tropical Hardwood SpeciesChamila Pathirana0Udayanthi Attanayake1Udula Dissanayake2Lakshman Gamlath3Kalyani Ketipearachchi4Terrence Madhujith5Janakie Eeswara6Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri LankaDepartment of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri LankaDepartment of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri LankaFruit Crop Research & Development Station, Gannoruwa, Peradeniya, Sri LankaFruit Crop Research & Development Station, Gannoruwa, Peradeniya, Sri LankaPostgraduate Institute of Agriculture, University of Peradeniya, Peradeniya 20400, Sri LankaDepartment of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya 20400, Sri LankaThe limited availability of high-quality planting material hinders the popularization of bael (Aegle marmelos (L.) Corr.) as a profitable cash crop in Sri Lanka. Five elite bael accessions, namely, Beheth Beli, Paragammana, Mawanella, Rambukkana, and Polonnaruwa-Supun, have been identified and used for mass propagation through budding and grafting. However, this process gets hampered by the limitations in large-scale production of planting material. Micropropagation is an alternative technique to produce clonal plants in large-scale; however, no established protocol is available for the field-grown elite bael accessions in Sri Lanka, and hence, the present study was conducted. The best sterilization method was found to be the washing of explants in a 2.5% fungicide solution for two hours. The full MS and ½MS media provided significantly similar performance in shooting, as indicated by the measured parameters. The twig plants did better than leaf explants concerning shoot extension, in which twig explants yield 1.33 cm taller shoots. The 1 mg/l of BAP concentration generated the highest number of shoots (6.20) and the most extended shoots (3.83 cm). The most successful rooting (60% success) was spotted with full MS, 1 mg/ml NAA, and 3% sugar. As previously reported, the successful micropropagation is possible if the explants are harvested from April to June, immediately after the fruiting season of the plant. The established protocol can mass-produce clonal bael plants from the elite accessions.http://dx.doi.org/10.1155/2020/8840386
collection DOAJ
language English
format Article
sources DOAJ
author Chamila Pathirana
Udayanthi Attanayake
Udula Dissanayake
Lakshman Gamlath
Kalyani Ketipearachchi
Terrence Madhujith
Janakie Eeswara
spellingShingle Chamila Pathirana
Udayanthi Attanayake
Udula Dissanayake
Lakshman Gamlath
Kalyani Ketipearachchi
Terrence Madhujith
Janakie Eeswara
Establishment of a Micropropagation Protocol for Elite Accessions of Bael (Aegle marmelos (L.) Corr.), a Tropical Hardwood Species
Advances in Agriculture
author_facet Chamila Pathirana
Udayanthi Attanayake
Udula Dissanayake
Lakshman Gamlath
Kalyani Ketipearachchi
Terrence Madhujith
Janakie Eeswara
author_sort Chamila Pathirana
title Establishment of a Micropropagation Protocol for Elite Accessions of Bael (Aegle marmelos (L.) Corr.), a Tropical Hardwood Species
title_short Establishment of a Micropropagation Protocol for Elite Accessions of Bael (Aegle marmelos (L.) Corr.), a Tropical Hardwood Species
title_full Establishment of a Micropropagation Protocol for Elite Accessions of Bael (Aegle marmelos (L.) Corr.), a Tropical Hardwood Species
title_fullStr Establishment of a Micropropagation Protocol for Elite Accessions of Bael (Aegle marmelos (L.) Corr.), a Tropical Hardwood Species
title_full_unstemmed Establishment of a Micropropagation Protocol for Elite Accessions of Bael (Aegle marmelos (L.) Corr.), a Tropical Hardwood Species
title_sort establishment of a micropropagation protocol for elite accessions of bael (aegle marmelos (l.) corr.), a tropical hardwood species
publisher Hindawi Limited
series Advances in Agriculture
issn 2356-654X
2314-7539
publishDate 2020-01-01
description The limited availability of high-quality planting material hinders the popularization of bael (Aegle marmelos (L.) Corr.) as a profitable cash crop in Sri Lanka. Five elite bael accessions, namely, Beheth Beli, Paragammana, Mawanella, Rambukkana, and Polonnaruwa-Supun, have been identified and used for mass propagation through budding and grafting. However, this process gets hampered by the limitations in large-scale production of planting material. Micropropagation is an alternative technique to produce clonal plants in large-scale; however, no established protocol is available for the field-grown elite bael accessions in Sri Lanka, and hence, the present study was conducted. The best sterilization method was found to be the washing of explants in a 2.5% fungicide solution for two hours. The full MS and ½MS media provided significantly similar performance in shooting, as indicated by the measured parameters. The twig plants did better than leaf explants concerning shoot extension, in which twig explants yield 1.33 cm taller shoots. The 1 mg/l of BAP concentration generated the highest number of shoots (6.20) and the most extended shoots (3.83 cm). The most successful rooting (60% success) was spotted with full MS, 1 mg/ml NAA, and 3% sugar. As previously reported, the successful micropropagation is possible if the explants are harvested from April to June, immediately after the fruiting season of the plant. The established protocol can mass-produce clonal bael plants from the elite accessions.
url http://dx.doi.org/10.1155/2020/8840386
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