Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis
Abstract Pretreatment is the key step to overcome the recalcitrance of lignocellulosic biomass making sugars available for subsequent enzymatic hydrolysis and microbial fermentation. During the process of pretreatment and enzymatic hydrolysis as well as fermentation, various toxic compounds may be g...
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doaj-0d41412e96a94b7eb9eb708fde95aa002020-11-24T23:21:41ZengSpringerOpenBioresources and Bioprocessing2197-43652018-02-015111210.1186/s40643-018-0193-9Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilisYongfu Yang0Mimi Hu1Ying Tang2Binan Geng3Mengyue Qiu4Qiaoning He5Shouwen Chen6Xia Wang7Shihui Yang8Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityAbstract Pretreatment is the key step to overcome the recalcitrance of lignocellulosic biomass making sugars available for subsequent enzymatic hydrolysis and microbial fermentation. During the process of pretreatment and enzymatic hydrolysis as well as fermentation, various toxic compounds may be generated with strong inhibition on cell growth and the metabolic capacity of fermenting strains. Zymomonas mobilis is a natural ethanologenic bacterium with many desirable industrial characteristics, but it can also be severely affected by lignocellulosic hydrolysate inhibitors. In this review, analytical methods to identify and quantify potential inhibitory compounds generated during lignocellulose pretreatment and enzymatic hydrolysis were discussed. The effect of hydrolysate inhibitors on Z. mobilis was also summarized as well as corresponding approaches especially the high-throughput ones for the evaluation. Then the strategies to enhance inhibitor tolerance of Z. mobilis were presented, which include both forward and reverse genetics approaches such as classical and novel mutagenesis approaches, adaptive laboratory evolution, as well as genetic and metabolic engineering. Moreover, this review provided perspectives and guidelines for future developments of robust strains for efficient bioethanol or biochemical production from lignocellulosic materials.http://link.springer.com/article/10.1186/s40643-018-0193-9LignocelluloseInhibitorZymomonas mobilisRobustnessAdaptive laboratory evolution (ALE)Mutagenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yongfu Yang Mimi Hu Ying Tang Binan Geng Mengyue Qiu Qiaoning He Shouwen Chen Xia Wang Shihui Yang |
spellingShingle |
Yongfu Yang Mimi Hu Ying Tang Binan Geng Mengyue Qiu Qiaoning He Shouwen Chen Xia Wang Shihui Yang Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis Bioresources and Bioprocessing Lignocellulose Inhibitor Zymomonas mobilis Robustness Adaptive laboratory evolution (ALE) Mutagenesis |
author_facet |
Yongfu Yang Mimi Hu Ying Tang Binan Geng Mengyue Qiu Qiaoning He Shouwen Chen Xia Wang Shihui Yang |
author_sort |
Yongfu Yang |
title |
Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis |
title_short |
Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis |
title_full |
Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis |
title_fullStr |
Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis |
title_full_unstemmed |
Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis |
title_sort |
progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in zymomonas mobilis |
publisher |
SpringerOpen |
series |
Bioresources and Bioprocessing |
issn |
2197-4365 |
publishDate |
2018-02-01 |
description |
Abstract Pretreatment is the key step to overcome the recalcitrance of lignocellulosic biomass making sugars available for subsequent enzymatic hydrolysis and microbial fermentation. During the process of pretreatment and enzymatic hydrolysis as well as fermentation, various toxic compounds may be generated with strong inhibition on cell growth and the metabolic capacity of fermenting strains. Zymomonas mobilis is a natural ethanologenic bacterium with many desirable industrial characteristics, but it can also be severely affected by lignocellulosic hydrolysate inhibitors. In this review, analytical methods to identify and quantify potential inhibitory compounds generated during lignocellulose pretreatment and enzymatic hydrolysis were discussed. The effect of hydrolysate inhibitors on Z. mobilis was also summarized as well as corresponding approaches especially the high-throughput ones for the evaluation. Then the strategies to enhance inhibitor tolerance of Z. mobilis were presented, which include both forward and reverse genetics approaches such as classical and novel mutagenesis approaches, adaptive laboratory evolution, as well as genetic and metabolic engineering. Moreover, this review provided perspectives and guidelines for future developments of robust strains for efficient bioethanol or biochemical production from lignocellulosic materials. |
topic |
Lignocellulose Inhibitor Zymomonas mobilis Robustness Adaptive laboratory evolution (ALE) Mutagenesis |
url |
http://link.springer.com/article/10.1186/s40643-018-0193-9 |
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