Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic Diversity
In this study, we evaluated the impact of the successive bioaugmentation of the activated sludge (AS) with the defined bacterial consortium on the activity and functional capacity of the AS microorganisms. In parallel, the removal of low concentrations of the selected non-steroidal anti-inflammatory...
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doaj-0e148ffe77fe46ec91befacccc19da872020-11-25T02:28:43ZengMDPI AGWater2073-44412020-04-01121133113310.3390/w12041133Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic DiversityJoanna Żur0Justyna Michalska1Artur Piński2Agnieszka Mrozik3Agnieszka Nowak4Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, PolandInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, PolandInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, PolandInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, PolandInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, PolandIn this study, we evaluated the impact of the successive bioaugmentation of the activated sludge (AS) with the defined bacterial consortium on the activity and functional capacity of the AS microorganisms. In parallel, the removal of low concentrations of the selected non-steroidal anti-inflammatory drugs (ibuprofen, naproxen, diclofenac) and analgesic paracetamol was studied. We found that the addition of the bacterial consortium consisting of three pharmaceuticals-degrading strains <i>Bacillus thuringiensis</i> B1 (2015b), <i>Stenotrophomonas maltophilia</i> KB2, and <i>Pseudomonas moorei</i> KB4 into the AS did not cause any significant changes in the biomass abundance and metabolic activity of the AS microorganisms. Although, the successive bioaugmentation of the AS caused a slight increase in the metabolic diversity, the intensity of carbohydrates usage, and metabolic richness. Microorganisms in the bioaugmented and non-bioaugmented AS were able to degrade the mixture of the analyzed drugs with similar efficiency, however, diclofenac was removed more effectively in the bioaugmented AS. Several metabolites were identified and efficiently utilized, with the exception of 4-OH diclofenac. Two new diclofenac-degrading strains assigned as <i>Serratia proteamaculans</i> AS4 and <i>Rahnella bruchi</i> AS7 were isolated from the diclofenac-treated AS.https://www.mdpi.com/2073-4441/12/4/1133activated sludgebacterial consortiumbioaugmentationbiodegradationfunctional capacitynon-steroidal anti-inflammatory drugs (NSAIDs) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joanna Żur Justyna Michalska Artur Piński Agnieszka Mrozik Agnieszka Nowak |
spellingShingle |
Joanna Żur Justyna Michalska Artur Piński Agnieszka Mrozik Agnieszka Nowak Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic Diversity Water activated sludge bacterial consortium bioaugmentation biodegradation functional capacity non-steroidal anti-inflammatory drugs (NSAIDs) |
author_facet |
Joanna Żur Justyna Michalska Artur Piński Agnieszka Mrozik Agnieszka Nowak |
author_sort |
Joanna Żur |
title |
Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic Diversity |
title_short |
Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic Diversity |
title_full |
Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic Diversity |
title_fullStr |
Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic Diversity |
title_full_unstemmed |
Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic Diversity |
title_sort |
effects of low concentration of selected analgesics and successive bioaugmentation of the activated sludge on its activity and metabolic diversity |
publisher |
MDPI AG |
series |
Water |
issn |
2073-4441 |
publishDate |
2020-04-01 |
description |
In this study, we evaluated the impact of the successive bioaugmentation of the activated sludge (AS) with the defined bacterial consortium on the activity and functional capacity of the AS microorganisms. In parallel, the removal of low concentrations of the selected non-steroidal anti-inflammatory drugs (ibuprofen, naproxen, diclofenac) and analgesic paracetamol was studied. We found that the addition of the bacterial consortium consisting of three pharmaceuticals-degrading strains <i>Bacillus thuringiensis</i> B1 (2015b), <i>Stenotrophomonas maltophilia</i> KB2, and <i>Pseudomonas moorei</i> KB4 into the AS did not cause any significant changes in the biomass abundance and metabolic activity of the AS microorganisms. Although, the successive bioaugmentation of the AS caused a slight increase in the metabolic diversity, the intensity of carbohydrates usage, and metabolic richness. Microorganisms in the bioaugmented and non-bioaugmented AS were able to degrade the mixture of the analyzed drugs with similar efficiency, however, diclofenac was removed more effectively in the bioaugmented AS. Several metabolites were identified and efficiently utilized, with the exception of 4-OH diclofenac. Two new diclofenac-degrading strains assigned as <i>Serratia proteamaculans</i> AS4 and <i>Rahnella bruchi</i> AS7 were isolated from the diclofenac-treated AS. |
topic |
activated sludge bacterial consortium bioaugmentation biodegradation functional capacity non-steroidal anti-inflammatory drugs (NSAIDs) |
url |
https://www.mdpi.com/2073-4441/12/4/1133 |
work_keys_str_mv |
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