Sequence-Based Discovery Demonstrates That Fixed Light Chain Human Transgenic Rats Produce a Diverse Repertoire of Antigen-Specific Antibodies

Sequence-Based Discovery Demonstrates That Fixed Light Chain Human Transgenic Rats Produce a Diverse Repertoire of Antigen-Specific Antibodies

We created a novel transgenic rat that expresses human antibodies comprising a diverse repertoire of heavy chains with a single common rearranged kappa light chain (IgKV3-15-JK1). This fixed light chain animal, called OmniFlic, presents a unique system for human therapeutic antibody discovery and a...

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Bibliographic Details
Main Authors: Katherine E. Harris, Shelley Force Aldred, Laura M. Davison, Heather Anne N. Ogana, Andrew Boudreau, Marianne Brüggemann, Michael Osborn, Biao Ma, Benjamin Buelow, Starlynn C. Clarke, Kevin H. Dang, Suhasini Iyer, Brett Jorgensen, Duy T. Pham, Payal P. Pratap, Udaya S. Rangaswamy, Ute Schellenberger, Wim C. van Schooten, Harshad S. Ugamraj, Omid Vafa, Roland Buelow, Nathan D. Trinklein
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-04-01
Series:Frontiers in Immunology
Subjects:
monoclonal human antibodies
transgenic rodent
rearranged light chain
somatic hypermutation
deep sequencing
antibody repertoire
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2018.00889/full

Internet

http://journal.frontiersin.org/article/10.3389/fimmu.2018.00889/full

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