Template preparation for rapid PCR in Colletotrichum lindemuthianum

Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic...

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Main Authors:	Roca M. Gabriela, Davide Lisete C., Wheals Alan E.
Format:	Article
Language:	English
Published:	Sociedade Brasileira de Microbiologia 2003-01-01
Series:	Brazilian Journal of Microbiology
Subjects:	anthracnose hygromycin resistance internal transcribed spacer rapid screening PCR methodology
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003

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spelling	doaj-0e78b7b4552d4bad921d52d4359b61462020-11-24T23:34:36ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052003-01-01341812Template preparation for rapid PCR in Colletotrichum lindemuthianumRoca M. GabrielaDavide Lisete C.Wheals Alan E.Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003anthracnosehygromycin resistanceinternal transcribed spacerrapid screeningPCR methodology
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Roca M. Gabriela Davide Lisete C. Wheals Alan E.
spellingShingle	Roca M. Gabriela Davide Lisete C. Wheals Alan E. Template preparation for rapid PCR in Colletotrichum lindemuthianum Brazilian Journal of Microbiology anthracnose hygromycin resistance internal transcribed spacer rapid screening PCR methodology
author_facet	Roca M. Gabriela Davide Lisete C. Wheals Alan E.
author_sort	Roca M. Gabriela
title	Template preparation for rapid PCR in Colletotrichum lindemuthianum
title_short	Template preparation for rapid PCR in Colletotrichum lindemuthianum
title_full	Template preparation for rapid PCR in Colletotrichum lindemuthianum
title_fullStr	Template preparation for rapid PCR in Colletotrichum lindemuthianum
title_full_unstemmed	Template preparation for rapid PCR in Colletotrichum lindemuthianum
title_sort	template preparation for rapid pcr in colletotrichum lindemuthianum
publisher	Sociedade Brasileira de Microbiologia
series	Brazilian Journal of Microbiology
issn	1517-8382 1678-4405
publishDate	2003-01-01
description	Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).
topic	anthracnose hygromycin resistance internal transcribed spacer rapid screening PCR methodology
url	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003
work_keys_str_mv	AT rocamgabriela templatepreparationforrapidpcrincolletotrichumlindemuthianum AT davidelisetec templatepreparationforrapidpcrincolletotrichumlindemuthianum AT whealsalane templatepreparationforrapidpcrincolletotrichumlindemuthianum
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Template preparation for rapid PCR in Colletotrichum lindemuthianum

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