High throughput single-cell detection of multiplex CRISPR-edited gene modifications

High throughput single-cell detection of multiplex CRISPR-edited gene modifications

Abstract CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells en...

Full description

Bibliographic Details
Main Authors: Elisa ten Hacken, Kendell Clement, Shuqiang Li, María Hernández-Sánchez, Robert Redd, Shu Wang, David Ruff, Michaela Gruber, Kaitlyn Baranowski, Jose Jacob, James Flynn, Keith W. Jones, Donna Neuberg, Kenneth J. Livak, Luca Pinello, Catherine J. Wu
Format: Article
Language:English
Published: BMC 2020-10-01
Series:Genome Biology
Subjects:
Genetics
Single cell
Genome editing
Loss-of-function
Mutation
CRISPR-Cas9
Online Access:http://link.springer.com/article/10.1186/s13059-020-02174-1
Description
Summary:Abstract CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence of Cas9 edits at single-cell resolution.
ISSN:1474-760X

Similar Items