Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia
Abstract Camels (Camelus dromedarius) are known to harbor multidrug resistant Gram-negative bacteria and to be involved in the transmission of various microorganisms to humans. Data on the occurrence of colistin resistant Escherichia coli as well as mobilized colistin resistance (mcr) genes in camel...
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doaj-1249b66ad9a748539fe22c25098d2cc72020-11-25T01:02:29ZengBMCActa Veterinaria Scandinavica1751-01472018-06-016011510.1186/s13028-018-0389-1Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern TunisiaMohamed Rhouma0Salma Bessalah1Imed Salhi2William Thériault3John Morris Fairbrother4Philippe Fravalo5Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of MontrealLivestock and Wildlife Laboratory, Arid Lands Institute (I.R.A), University of GabèsLivestock and Wildlife Laboratory, Arid Lands Institute (I.R.A), University of GabèsDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine, University of MontrealDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine, University of MontrealDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine, University of MontrealAbstract Camels (Camelus dromedarius) are known to harbor multidrug resistant Gram-negative bacteria and to be involved in the transmission of various microorganisms to humans. Data on the occurrence of colistin resistant Escherichia coli as well as mobilized colistin resistance (mcr) genes in camels are lacking. We investigated the presence of colistin resistance and mcr (1–2) genes in E. coli from the feces of camels in Tunisia. Presumptive E. coli isolates from camel-calves in southern Tunisia were qualitatively screened for growth on Mueller–Hinton agar supplemented with 2 mg/L of colistin. The minimal inhibitory concentration of colistin was determined for isolates growing on this medium. All isolates were screened for the presence of the mcr-1 and mcr-2 genes by polymerase chain reaction without detecting any of these genes. However, one isolate was confirmed resistant to colistin and further testing of this isolate revealed it to be Enterobacter cloacae. Our study demonstrated absence of colistin resistance and of the mcr-1 and mcr-2 genes in E. coli isolated from camel feces in southern Tunisia. Thus, there is no evidence that camels represent a major source of mcr genes contamination for the local population or for tourists visiting southern Tunisia.http://link.springer.com/article/10.1186/s13028-018-0389-1CamelColistin resistanceE. coliHumanMCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohamed Rhouma Salma Bessalah Imed Salhi William Thériault John Morris Fairbrother Philippe Fravalo |
spellingShingle |
Mohamed Rhouma Salma Bessalah Imed Salhi William Thériault John Morris Fairbrother Philippe Fravalo Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia Acta Veterinaria Scandinavica Camel Colistin resistance E. coli Human MCR |
author_facet |
Mohamed Rhouma Salma Bessalah Imed Salhi William Thériault John Morris Fairbrother Philippe Fravalo |
author_sort |
Mohamed Rhouma |
title |
Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia |
title_short |
Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia |
title_full |
Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia |
title_fullStr |
Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia |
title_full_unstemmed |
Screening for fecal presence of colistin-resistant Escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern Tunisia |
title_sort |
screening for fecal presence of colistin-resistant escherichia coli and mcr-1 and mcr-2 genes in camel-calves in southern tunisia |
publisher |
BMC |
series |
Acta Veterinaria Scandinavica |
issn |
1751-0147 |
publishDate |
2018-06-01 |
description |
Abstract Camels (Camelus dromedarius) are known to harbor multidrug resistant Gram-negative bacteria and to be involved in the transmission of various microorganisms to humans. Data on the occurrence of colistin resistant Escherichia coli as well as mobilized colistin resistance (mcr) genes in camels are lacking. We investigated the presence of colistin resistance and mcr (1–2) genes in E. coli from the feces of camels in Tunisia. Presumptive E. coli isolates from camel-calves in southern Tunisia were qualitatively screened for growth on Mueller–Hinton agar supplemented with 2 mg/L of colistin. The minimal inhibitory concentration of colistin was determined for isolates growing on this medium. All isolates were screened for the presence of the mcr-1 and mcr-2 genes by polymerase chain reaction without detecting any of these genes. However, one isolate was confirmed resistant to colistin and further testing of this isolate revealed it to be Enterobacter cloacae. Our study demonstrated absence of colistin resistance and of the mcr-1 and mcr-2 genes in E. coli isolated from camel feces in southern Tunisia. Thus, there is no evidence that camels represent a major source of mcr genes contamination for the local population or for tourists visiting southern Tunisia. |
topic |
Camel Colistin resistance E. coli Human MCR |
url |
http://link.springer.com/article/10.1186/s13028-018-0389-1 |
work_keys_str_mv |
AT mohamedrhouma screeningforfecalpresenceofcolistinresistantescherichiacoliandmcr1andmcr2genesincamelcalvesinsoutherntunisia AT salmabessalah screeningforfecalpresenceofcolistinresistantescherichiacoliandmcr1andmcr2genesincamelcalvesinsoutherntunisia AT imedsalhi screeningforfecalpresenceofcolistinresistantescherichiacoliandmcr1andmcr2genesincamelcalvesinsoutherntunisia AT williamtheriault screeningforfecalpresenceofcolistinresistantescherichiacoliandmcr1andmcr2genesincamelcalvesinsoutherntunisia AT johnmorrisfairbrother screeningforfecalpresenceofcolistinresistantescherichiacoliandmcr1andmcr2genesincamelcalvesinsoutherntunisia AT philippefravalo screeningforfecalpresenceofcolistinresistantescherichiacoliandmcr1andmcr2genesincamelcalvesinsoutherntunisia |
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