Biotinylated θ-toxin derivative as a probe to examine intracellular cholesterol-rich domains in normal and Niemann-Pick type C1 cells

Biotinylated θ-toxin derivative as a probe to examine intracellular cholesterol-rich domains in normal and Niemann-Pick type C1 cells

BCθ is a proteolytically nicked and biotinylated derivative of a cholesterol binding protein perfringolysin O (θ-toxin), and has been used to detect cholesterol-rich domains at the plasma membrane (PM). Here we show that by modifying the cell fixation condition, BCθ can also be used to detect choles...

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Bibliographic Details
Main Authors: Shigeki Sugii, Patrick C. Reid, Nobutaka Ohgami, Yukiko Shimada, Robert A. Maue, Haruaki Ninomiya, Yoshiko Ohno-Iwashita, Ta-Yuan Chang
Format: Article
Language:English
Published: Elsevier 2003-05-01
Series:Journal of Lipid Research
Subjects:
BC θ
perfringolysin O toxin
cholesterol metabolism
cholesterol stain
intracellular cholesterol trafficking
cholesterol mutants
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520311494

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http://www.sciencedirect.com/science/article/pii/S0022227520311494

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