Establishment of a Transformation Coupled in vitro End Joining Assay to Estimate Radiosensitivity in Tumor Cells

Establishment of a Transformation Coupled in vitro End Joining Assay to Estimate Radiosensitivity in Tumor Cells

Here, we present a modified in vitro end-joining (EJ) assay to quantify EJ capacity, accuracy as well as pathway switch to alternative end-joining (Alt-EJ) or single strand annealing (SSA). A novel transformation assay was established to specifically measure circular repair products, which correlate...

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Bibliographic Details
Main Authors: Sarah Degenhardt, Kristin Dreffke, Urlike Schötz, Cordula Petersen, Rita Engenhart-Cabillic, Kai Rothkamm, Jochen Dahm-Daphi, Ekkehard Dikomey, Wael Yassin Mansour
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Oncology
Subjects:
in vitro end joining assay
DSB repair pathway choice
radiosensitivity
HNSC
head and neck squamous cell carcinoma
classical NHEJ
Online Access:https://www.frontiersin.org/article/10.3389/fonc.2020.01480/full

Internet

https://www.frontiersin.org/article/10.3389/fonc.2020.01480/full

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