Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures

RAD51 and DMC1 recombinases catalyse high-fidelity and mismatch tolerant recombination, processes that are indispensable for the maintenance of genomic integrity. Here, the authors via cryo-EM, molecular dynamics simulation and functional analysis elucidate the structural difference between RAD51 an...

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Main Authors:	Shih-Chi Luo, Hsin-Yi Yeh, Wei-Hsuan Lan, Yi-Min Wu, Cheng-Han Yang, Hao-Yen Chang, Guan-Chin Su, Chia-Yi Lee, Wen-Jin Wu, Hung-Wen Li, Meng-Chiao Ho, Peter Chi, Ming-Daw Tsai
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-01-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-20258-1

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spelling	doaj-14e414a2f00d459ea2add7b10358a4162021-01-17T12:12:56ZengNature Publishing GroupNature Communications2041-17232021-01-0112111010.1038/s41467-020-20258-1Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structuresShih-Chi Luo0Hsin-Yi Yeh1Wei-Hsuan Lan2Yi-Min Wu3Cheng-Han Yang4Hao-Yen Chang5Guan-Chin Su6Chia-Yi Lee7Wen-Jin Wu8Hung-Wen Li9Meng-Chiao Ho10Peter Chi11Ming-Daw Tsai12Institute of Biological Chemistry, Academia SinicaInstitute of Biochemical Sciences, National Taiwan UniversityDepartment of Chemistry, National Taiwan UniversityInstitute of Biological Chemistry, Academia SinicaInstitute of Biological Chemistry, Academia SinicaInstitute of Biochemical Sciences, National Taiwan UniversityInstitute of Biochemical Sciences, National Taiwan UniversityInstitute of Biochemical Sciences, National Taiwan UniversityInstitute of Biological Chemistry, Academia SinicaDepartment of Chemistry, National Taiwan UniversityInstitute of Biological Chemistry, Academia SinicaInstitute of Biological Chemistry, Academia SinicaInstitute of Biological Chemistry, Academia SinicaRAD51 and DMC1 recombinases catalyse high-fidelity and mismatch tolerant recombination, processes that are indispensable for the maintenance of genomic integrity. Here, the authors via cryo-EM, molecular dynamics simulation and functional analysis elucidate the structural difference between RAD51 and DMC1 with regard to mismatch tolerance.https://doi.org/10.1038/s41467-020-20258-1
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Shih-Chi Luo Hsin-Yi Yeh Wei-Hsuan Lan Yi-Min Wu Cheng-Han Yang Hao-Yen Chang Guan-Chin Su Chia-Yi Lee Wen-Jin Wu Hung-Wen Li Meng-Chiao Ho Peter Chi Ming-Daw Tsai
spellingShingle	Shih-Chi Luo Hsin-Yi Yeh Wei-Hsuan Lan Yi-Min Wu Cheng-Han Yang Hao-Yen Chang Guan-Chin Su Chia-Yi Lee Wen-Jin Wu Hung-Wen Li Meng-Chiao Ho Peter Chi Ming-Daw Tsai Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures Nature Communications
author_facet	Shih-Chi Luo Hsin-Yi Yeh Wei-Hsuan Lan Yi-Min Wu Cheng-Han Yang Hao-Yen Chang Guan-Chin Su Chia-Yi Lee Wen-Jin Wu Hung-Wen Li Meng-Chiao Ho Peter Chi Ming-Daw Tsai
author_sort	Shih-Chi Luo
title	Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures
title_short	Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures
title_full	Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures
title_fullStr	Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures
title_full_unstemmed	Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures
title_sort	identification of fidelity-governing factors in human recombinases dmc1 and rad51 from cryo-em structures
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-01-01
description	RAD51 and DMC1 recombinases catalyse high-fidelity and mismatch tolerant recombination, processes that are indispensable for the maintenance of genomic integrity. Here, the authors via cryo-EM, molecular dynamics simulation and functional analysis elucidate the structural difference between RAD51 and DMC1 with regard to mismatch tolerance.
url	https://doi.org/10.1038/s41467-020-20258-1
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Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures

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