Antimicrobial efficacy of the EndoVac system plus PDT against intracanal Candida albicans: an ex vivo study
This study evaluated the ex vivoantimicrobial efficacy of the EndoVac system and the photodynamic therapy (PDT) associated with chemomechanical debridement (CMD) and intracanal medication on Candida albicans. Seventy-eight sterile premolars were contaminated withC. albicans (ATCC 21433) for 30 days....
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Sociedade Brasileira de Pesquisa Odontológica
2015-01-01
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doaj-180eeb3685394cd4b68704bea08dfe0f2020-11-24T20:43:54ZengSociedade Brasileira de Pesquisa Odontológica Brazilian Oral Research1807-31072015-01-012911710.1590/1807-3107BOR-2015.vol29.0129S1806-83242015000100308Antimicrobial efficacy of the EndoVac system plus PDT against intracanal Candida albicans: an ex vivo studyRachel Garcia de MIRANDAHeloisa Dell Santo GUSMANAna Paula Vieira COLOMBOThis study evaluated the ex vivoantimicrobial efficacy of the EndoVac system and the photodynamic therapy (PDT) associated with chemomechanical debridement (CMD) and intracanal medication on Candida albicans. Seventy-eight sterile premolars were contaminated withC. albicans (ATCC 21433) for 30 days. The teeth were randomly assigned into four groups: Control (CMD with conventional irrigation); Endovac (CMD with EndoVac system); PDT (CMD with conventional irrigation and PDT); and Endovac + PDT (CMD with EndoVac and PDT). After the therapies, intracanal dressing (calcium hydroxide) was applied to all teeth for seven days. Samples were obtained before (T1) and after the therapeutic procedures (T2), and after intracanal medication (T3), plated onto BHI agar and incubated (37°C, 48 h) to determine the colony-forming units (CFU)/mL. The overall mean level ofC. albicans at baseline was relatively high (1.85 x 106 ± 2.7 x 106 CFU mL-1). A significant reduction of C. albicans(p < 0.05) was observed over time (T1 to T2 and T1 to T3) in all groups. An additional significant reduction from T2 to T3 was observed only in the Endovac group (p < 0.05). No differences in mean reduction of C. albicans were observed among groups. However, the Endovac group presented the lowest mean counts of C. albicans at T3, whereas the PDT group had the highest counts of this microorganism (p < 0.05). The EndoVac system of irrigation/aspiration associated with CMD was the most effective therapeutic protocol for reducing intracanal levels of C. albicans. PDT showed a very limited efficacy against this species.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100308&lng=en&tlng=enRoot Canal TherapyPhotochemotherapyCandida AlbicansCalcium HydroxideSodium Hypochlorite |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rachel Garcia de MIRANDA Heloisa Dell Santo GUSMAN Ana Paula Vieira COLOMBO |
spellingShingle |
Rachel Garcia de MIRANDA Heloisa Dell Santo GUSMAN Ana Paula Vieira COLOMBO Antimicrobial efficacy of the EndoVac system plus PDT against intracanal Candida albicans: an ex vivo study Brazilian Oral Research Root Canal Therapy Photochemotherapy Candida Albicans Calcium Hydroxide Sodium Hypochlorite |
author_facet |
Rachel Garcia de MIRANDA Heloisa Dell Santo GUSMAN Ana Paula Vieira COLOMBO |
author_sort |
Rachel Garcia de MIRANDA |
title |
Antimicrobial efficacy of the EndoVac system plus PDT against intracanal Candida albicans: an ex vivo study |
title_short |
Antimicrobial efficacy of the EndoVac system plus PDT against intracanal Candida albicans: an ex vivo study |
title_full |
Antimicrobial efficacy of the EndoVac system plus PDT against intracanal Candida albicans: an ex vivo study |
title_fullStr |
Antimicrobial efficacy of the EndoVac system plus PDT against intracanal Candida albicans: an ex vivo study |
title_full_unstemmed |
Antimicrobial efficacy of the EndoVac system plus PDT against intracanal Candida albicans: an ex vivo study |
title_sort |
antimicrobial efficacy of the endovac system plus pdt against intracanal candida albicans: an ex vivo study |
publisher |
Sociedade Brasileira de Pesquisa Odontológica |
series |
Brazilian Oral Research |
issn |
1807-3107 |
publishDate |
2015-01-01 |
description |
This study evaluated the ex vivoantimicrobial efficacy of the EndoVac system and the photodynamic therapy (PDT) associated with chemomechanical debridement (CMD) and intracanal medication on Candida albicans. Seventy-eight sterile premolars were contaminated withC. albicans (ATCC 21433) for 30 days. The teeth were randomly assigned into four groups: Control (CMD with conventional irrigation); Endovac (CMD with EndoVac system); PDT (CMD with conventional irrigation and PDT); and Endovac + PDT (CMD with EndoVac and PDT). After the therapies, intracanal dressing (calcium hydroxide) was applied to all teeth for seven days. Samples were obtained before (T1) and after the therapeutic procedures (T2), and after intracanal medication (T3), plated onto BHI agar and incubated (37°C, 48 h) to determine the colony-forming units (CFU)/mL. The overall mean level ofC. albicans at baseline was relatively high (1.85 x 106 ± 2.7 x 106 CFU mL-1). A significant reduction of C. albicans(p < 0.05) was observed over time (T1 to T2 and T1 to T3) in all groups. An additional significant reduction from T2 to T3 was observed only in the Endovac group (p < 0.05). No differences in mean reduction of C. albicans were observed among groups. However, the Endovac group presented the lowest mean counts of C. albicans at T3, whereas the PDT group had the highest counts of this microorganism (p < 0.05). The EndoVac system of irrigation/aspiration associated with CMD was the most effective therapeutic protocol for reducing intracanal levels of C. albicans. PDT showed a very limited efficacy against this species. |
topic |
Root Canal Therapy Photochemotherapy Candida Albicans Calcium Hydroxide Sodium Hypochlorite |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100308&lng=en&tlng=en |
work_keys_str_mv |
AT rachelgarciademiranda antimicrobialefficacyoftheendovacsystempluspdtagainstintracanalcandidaalbicansanexvivostudy AT heloisadellsantogusman antimicrobialefficacyoftheendovacsystempluspdtagainstintracanalcandidaalbicansanexvivostudy AT anapaulavieiracolombo antimicrobialefficacyoftheendovacsystempluspdtagainstintracanalcandidaalbicansanexvivostudy |
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