Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China
We conducted a large-scale epidemiological investigation to detect the prevalence of Toxoplasma gondii in four marine bivalve shellfish species collected from six representative coastal regions of Weihai, eastern China. Between January 2018 and December 2018, 14,535 marine bivalve shellfish pooled i...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2021-04-01
|
Series: | Ecotoxicology and Environmental Safety |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321001597 |
id |
doaj-19fb9f67017043bb97e5600a5bd646de |
---|---|
record_format |
Article |
spelling |
doaj-19fb9f67017043bb97e5600a5bd646de2021-04-23T06:16:34ZengElsevierEcotoxicology and Environmental Safety0147-65132021-04-01213112048Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern ChinaWei Cong0Man-Yao Li1Yang Zou2Jun-Yang Ma3Bo Wang4Zhao-Yang Jiang5Hany M. Elsheikha6Marine College, Shandong University, Weihai, Shandong, 264209, PR ChinaMarine College, Shandong University, Weihai, Shandong, 264209, PR ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, College of Veterinary Medicine, Northeast Agricultural University, 600 Changjiang Street, Harbin 150030, PR ChinaMarine College, Shandong University, Weihai, Shandong, 264209, PR ChinaSchool of Mathematics and Actuarial Science, University of Leicester, Leicester LE1 7RH, UKMarine College, Shandong University, Weihai, Shandong, 264209, PR China; Corresponding authors.Faculty of Medicine and Health Sciences, School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, UK; Corresponding authors.We conducted a large-scale epidemiological investigation to detect the prevalence of Toxoplasma gondii in four marine bivalve shellfish species collected from six representative coastal regions of Weihai, eastern China. Between January 2018 and December 2018, 14,535 marine bivalve shellfish pooled into 2907 samples were randomly collected and examined for T. gondii DNA by a nested PCR assay targeting B1 gene. The results showed that 2.8% (82) of the 2907 pooled samples were tested positive for T. gondii DNA. Two T. gondii genotype (ToxoDB Genotype #9 and ToxoDB Genotype #1) were identified PCR-restriction fragment length polymorphism analysis. Factors that were found significantly associated with the presence of T. gondii DNA in marine bivalve shellfish included the source of samples (being wild) (odds ratio [OR], 3.34; 95% confidence interval [CI], 2.00–5.84; p < 0.01), surface runoff near the sampling site (OR, 2.64; 95% CI, 1.47–4.72; p < 0.01), and presence of cats near the sampling site (OR, 1.77; 95% CI, 1.02–3.07; p = 0.04). Moreover, the prevalence of T. gondii DNA in marine bivalve shellfish correlated with temperature (Pearson’s correlation: R = 0.75, p = 0.0049) and precipitation (R = 0.87, p = 0.00021). These findings provide new insights into the presence of T. gondii DNA in marine bivalve shellfish and highlight the impact of human activity on marine pollution by such an important terrestrial pathogen pollutant.http://www.sciencedirect.com/science/article/pii/S0147651321001597Toxoplasma gondiiMarine bivalve shellfishBiomonitoringZoonosisChina |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Cong Man-Yao Li Yang Zou Jun-Yang Ma Bo Wang Zhao-Yang Jiang Hany M. Elsheikha |
spellingShingle |
Wei Cong Man-Yao Li Yang Zou Jun-Yang Ma Bo Wang Zhao-Yang Jiang Hany M. Elsheikha Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China Ecotoxicology and Environmental Safety Toxoplasma gondii Marine bivalve shellfish Biomonitoring Zoonosis China |
author_facet |
Wei Cong Man-Yao Li Yang Zou Jun-Yang Ma Bo Wang Zhao-Yang Jiang Hany M. Elsheikha |
author_sort |
Wei Cong |
title |
Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China |
title_short |
Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China |
title_full |
Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China |
title_fullStr |
Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China |
title_full_unstemmed |
Prevalence, genotypes and risk factors for Toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern China |
title_sort |
prevalence, genotypes and risk factors for toxoplasma gondii contamination in marine bivalve shellfish in offshore waters in eastern china |
publisher |
Elsevier |
series |
Ecotoxicology and Environmental Safety |
issn |
0147-6513 |
publishDate |
2021-04-01 |
description |
We conducted a large-scale epidemiological investigation to detect the prevalence of Toxoplasma gondii in four marine bivalve shellfish species collected from six representative coastal regions of Weihai, eastern China. Between January 2018 and December 2018, 14,535 marine bivalve shellfish pooled into 2907 samples were randomly collected and examined for T. gondii DNA by a nested PCR assay targeting B1 gene. The results showed that 2.8% (82) of the 2907 pooled samples were tested positive for T. gondii DNA. Two T. gondii genotype (ToxoDB Genotype #9 and ToxoDB Genotype #1) were identified PCR-restriction fragment length polymorphism analysis. Factors that were found significantly associated with the presence of T. gondii DNA in marine bivalve shellfish included the source of samples (being wild) (odds ratio [OR], 3.34; 95% confidence interval [CI], 2.00–5.84; p < 0.01), surface runoff near the sampling site (OR, 2.64; 95% CI, 1.47–4.72; p < 0.01), and presence of cats near the sampling site (OR, 1.77; 95% CI, 1.02–3.07; p = 0.04). Moreover, the prevalence of T. gondii DNA in marine bivalve shellfish correlated with temperature (Pearson’s correlation: R = 0.75, p = 0.0049) and precipitation (R = 0.87, p = 0.00021). These findings provide new insights into the presence of T. gondii DNA in marine bivalve shellfish and highlight the impact of human activity on marine pollution by such an important terrestrial pathogen pollutant. |
topic |
Toxoplasma gondii Marine bivalve shellfish Biomonitoring Zoonosis China |
url |
http://www.sciencedirect.com/science/article/pii/S0147651321001597 |
work_keys_str_mv |
AT weicong prevalencegenotypesandriskfactorsfortoxoplasmagondiicontaminationinmarinebivalveshellfishinoffshorewatersineasternchina AT manyaoli prevalencegenotypesandriskfactorsfortoxoplasmagondiicontaminationinmarinebivalveshellfishinoffshorewatersineasternchina AT yangzou prevalencegenotypesandriskfactorsfortoxoplasmagondiicontaminationinmarinebivalveshellfishinoffshorewatersineasternchina AT junyangma prevalencegenotypesandriskfactorsfortoxoplasmagondiicontaminationinmarinebivalveshellfishinoffshorewatersineasternchina AT bowang prevalencegenotypesandriskfactorsfortoxoplasmagondiicontaminationinmarinebivalveshellfishinoffshorewatersineasternchina AT zhaoyangjiang prevalencegenotypesandriskfactorsfortoxoplasmagondiicontaminationinmarinebivalveshellfishinoffshorewatersineasternchina AT hanymelsheikha prevalencegenotypesandriskfactorsfortoxoplasmagondiicontaminationinmarinebivalveshellfishinoffshorewatersineasternchina |
_version_ |
1721512962845310976 |