Physiological responses of Brassica napus to fulvic acid under water stress: Chlorophyll a fluorescence and antioxidant enzyme activity
The ameliorative effect of fulvic acid (0, 300, and 600 mg L− 1) on photosystem II and antioxidant enzyme activity of the rapeseed (Brassica napus L.) plant under water stress (60, 100, and 140 mm evaporation from class A pan) was studied using split plots in a randomized complete block design with...
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doaj-1c0257dccbcc43c6bdcc5f280178d68c2021-02-02T00:49:03ZengKeAi Communications Co., Ltd.Crop Journal2095-54212214-51412015-10-013543443910.1016/j.cj.2015.05.006Physiological responses of Brassica napus to fulvic acid under water stress: Chlorophyll a fluorescence and antioxidant enzyme activityRamin Lotfi0Mohammad Pessarakli1Puriya Gharavi-Kouchebagh2Hossein Khoshvaghti3Department of Plant Eco-physiology, Faculty of Agriculture, University of Tabriz, Tabriz, IranSchool of Plant Science, The University of Arizona, Tucson, AZ, USADepartment of Plant Eco-physiology, Faculty of Agriculture, University of Tabriz, Tabriz, IranDepartment of Agriculture, Payame Noor University (PNU), IranThe ameliorative effect of fulvic acid (0, 300, and 600 mg L− 1) on photosystem II and antioxidant enzyme activity of the rapeseed (Brassica napus L.) plant under water stress (60, 100, and 140 mm evaporation from class A pan) was studied using split plots in a randomized complete block design with three replications. Results indicated that application of fulvic acid (FA) improved the maximum quantum efficiency of PSII (Fv/Fm) and performance index (PI) of plants under both well-watered and limited-water conditions. The time span from Fo to Fm and the energy necessary for the closure of all reaction centers was significantly increased, but the size of the plastoquinone pool was reduced with increasing water stress levels. Plants treated with FA had higher peroxidase and catalase activities under all irrigation conditions. Activities of ascorbate peroxidase and superoxide dismutase in plants increased with increasing water stress. Malondialdehyde increased under severe water stress, but application of FA significantly decreased lipid peroxidation. Production of reactive oxygen species (ROS) is a common phenomenon in plants under stress. Under this condition, the balance between the production of ROS and the quenching activity of antioxidants is upset, often resulting in oxidative damage. In this study, application of FA significantly increased fluorescence of chlorophyll a, inhibiting ROS production and enhancing antioxidant enzymes activity that destroyed ROS. Thus, ROS in plant cells was reduced under water stress by application of FA and consequently lipid peroxidation was reduced.http://www.sciencedirect.com/science/article/pii/S2214514115000744Brassica napusFluorescenceAntioxidant enzymeWater stressFulvic acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ramin Lotfi Mohammad Pessarakli Puriya Gharavi-Kouchebagh Hossein Khoshvaghti |
spellingShingle |
Ramin Lotfi Mohammad Pessarakli Puriya Gharavi-Kouchebagh Hossein Khoshvaghti Physiological responses of Brassica napus to fulvic acid under water stress: Chlorophyll a fluorescence and antioxidant enzyme activity Crop Journal Brassica napus Fluorescence Antioxidant enzyme Water stress Fulvic acid |
author_facet |
Ramin Lotfi Mohammad Pessarakli Puriya Gharavi-Kouchebagh Hossein Khoshvaghti |
author_sort |
Ramin Lotfi |
title |
Physiological responses of Brassica napus to fulvic acid under water stress: Chlorophyll a fluorescence and antioxidant enzyme activity |
title_short |
Physiological responses of Brassica napus to fulvic acid under water stress: Chlorophyll a fluorescence and antioxidant enzyme activity |
title_full |
Physiological responses of Brassica napus to fulvic acid under water stress: Chlorophyll a fluorescence and antioxidant enzyme activity |
title_fullStr |
Physiological responses of Brassica napus to fulvic acid under water stress: Chlorophyll a fluorescence and antioxidant enzyme activity |
title_full_unstemmed |
Physiological responses of Brassica napus to fulvic acid under water stress: Chlorophyll a fluorescence and antioxidant enzyme activity |
title_sort |
physiological responses of brassica napus to fulvic acid under water stress: chlorophyll a fluorescence and antioxidant enzyme activity |
publisher |
KeAi Communications Co., Ltd. |
series |
Crop Journal |
issn |
2095-5421 2214-5141 |
publishDate |
2015-10-01 |
description |
The ameliorative effect of fulvic acid (0, 300, and 600 mg L− 1) on photosystem II and antioxidant enzyme activity of the rapeseed (Brassica napus L.) plant under water stress (60, 100, and 140 mm evaporation from class A pan) was studied using split plots in a randomized complete block design with three replications. Results indicated that application of fulvic acid (FA) improved the maximum quantum efficiency of PSII (Fv/Fm) and performance index (PI) of plants under both well-watered and limited-water conditions. The time span from Fo to Fm and the energy necessary for the closure of all reaction centers was significantly increased, but the size of the plastoquinone pool was reduced with increasing water stress levels. Plants treated with FA had higher peroxidase and catalase activities under all irrigation conditions. Activities of ascorbate peroxidase and superoxide dismutase in plants increased with increasing water stress. Malondialdehyde increased under severe water stress, but application of FA significantly decreased lipid peroxidation. Production of reactive oxygen species (ROS) is a common phenomenon in plants under stress. Under this condition, the balance between the production of ROS and the quenching activity of antioxidants is upset, often resulting in oxidative damage. In this study, application of FA significantly increased fluorescence of chlorophyll a, inhibiting ROS production and enhancing antioxidant enzymes activity that destroyed ROS. Thus, ROS in plant cells was reduced under water stress by application of FA and consequently lipid peroxidation was reduced. |
topic |
Brassica napus Fluorescence Antioxidant enzyme Water stress Fulvic acid |
url |
http://www.sciencedirect.com/science/article/pii/S2214514115000744 |
work_keys_str_mv |
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