Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial

Abstract Probiotics have provided benefits to general health, but they are still insufficient to dental health. Objective: This study aimed to evaluate milk supplemented with probiotic bacteria and standard milk, measured by levels of Streptococcus mutans (S. mutans) and Lactobacillus spp., in 3-4-...

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Main Authors: Judy Villavicencio, Lina Maria Villegas, Maria Cristina Arango, Susana Arias, Francia Triana
Format: Article
Language:English
Published: University of São Paulo 2018-05-01
Series:Journal of Applied Oral Science
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100454&lng=en&tlng=en
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spelling doaj-1ebc15044faf40ec8ab11197441bdbe92020-11-24T23:48:08ZengUniversity of São PauloJournal of Applied Oral Science1678-77652018-05-0126010.1590/1678-7757-2017-0318S1678-77572018000100454Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trialJudy VillavicencioLina Maria VillegasMaria Cristina ArangoSusana AriasFrancia TrianaAbstract Probiotics have provided benefits to general health, but they are still insufficient to dental health. Objective: This study aimed to evaluate milk supplemented with probiotic bacteria and standard milk, measured by levels of Streptococcus mutans (S. mutans) and Lactobacillus spp., in 3-4-year-old children after 9 months of intervention. Material and Methods: The study was a triple-blind, placebo-controlled, randomized trial. The sample was composed of 363 preschoolers attending five child development centers in Cali, Colombia. They were randomized to two groups: children in the intervention group drank 200 mL of milk with Lactobacillus rhamnosus 5x106 and Bifidobacteruim longum 3x106, and children in the control group drank 200 mL of standard milk. Interventions occurred on weekdays and information was gathered through scheduled clinical examination. The primary result was the number of colony forming units (CFU) of S. mutans and Lactobacillus spp. in the saliva. Secondary results were dental caries, rated by the International Caries Detection and Assessment System (ICDAS), dental plaque, pH, and salivary buffer capacity. Results: The proportion of S. mutans was lower in the intervention group compared with the control group after 9 months; however, the differences did not reach statistical significance (p=0.173); on the other hand, statistically significant differences between groups were found in the CFU/mL of Lactobacillus spp. (p=0.002). There was not statistically significant difference in the prevalence of dental caries for both groups (p=0.767). Differences between groups were found in the salivary buffering capacity (p=0.000); neither salivary pH nor dental plaque were significantly different. Conclusions: Regular consumption of milk containing probiotics bacteria reduced CFU/mL of Lactobacillus spp. and increased salivary buffering capacity at 9 months of consumption.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100454&lng=en&tlng=enPreschool childrenProbioticsLactobacillus rhamnosusBifidobacterium longumStreptococcus mutansLactobacillus sppCaries preventionEarly childhood cariesClinical trial
collection DOAJ
language English
format Article
sources DOAJ
author Judy Villavicencio
Lina Maria Villegas
Maria Cristina Arango
Susana Arias
Francia Triana
spellingShingle Judy Villavicencio
Lina Maria Villegas
Maria Cristina Arango
Susana Arias
Francia Triana
Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial
Journal of Applied Oral Science
Preschool children
Probiotics
Lactobacillus rhamnosus
Bifidobacterium longum
Streptococcus mutans
Lactobacillus spp
Caries prevention
Early childhood caries
Clinical trial
author_facet Judy Villavicencio
Lina Maria Villegas
Maria Cristina Arango
Susana Arias
Francia Triana
author_sort Judy Villavicencio
title Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial
title_short Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial
title_full Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial
title_fullStr Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial
title_full_unstemmed Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial
title_sort effects of a food enriched with probiotics on streptococcus mutans and lactobacillus spp. salivary counts in preschool children: a cluster randomized trial
publisher University of São Paulo
series Journal of Applied Oral Science
issn 1678-7765
publishDate 2018-05-01
description Abstract Probiotics have provided benefits to general health, but they are still insufficient to dental health. Objective: This study aimed to evaluate milk supplemented with probiotic bacteria and standard milk, measured by levels of Streptococcus mutans (S. mutans) and Lactobacillus spp., in 3-4-year-old children after 9 months of intervention. Material and Methods: The study was a triple-blind, placebo-controlled, randomized trial. The sample was composed of 363 preschoolers attending five child development centers in Cali, Colombia. They were randomized to two groups: children in the intervention group drank 200 mL of milk with Lactobacillus rhamnosus 5x106 and Bifidobacteruim longum 3x106, and children in the control group drank 200 mL of standard milk. Interventions occurred on weekdays and information was gathered through scheduled clinical examination. The primary result was the number of colony forming units (CFU) of S. mutans and Lactobacillus spp. in the saliva. Secondary results were dental caries, rated by the International Caries Detection and Assessment System (ICDAS), dental plaque, pH, and salivary buffer capacity. Results: The proportion of S. mutans was lower in the intervention group compared with the control group after 9 months; however, the differences did not reach statistical significance (p=0.173); on the other hand, statistically significant differences between groups were found in the CFU/mL of Lactobacillus spp. (p=0.002). There was not statistically significant difference in the prevalence of dental caries for both groups (p=0.767). Differences between groups were found in the salivary buffering capacity (p=0.000); neither salivary pH nor dental plaque were significantly different. Conclusions: Regular consumption of milk containing probiotics bacteria reduced CFU/mL of Lactobacillus spp. and increased salivary buffering capacity at 9 months of consumption.
topic Preschool children
Probiotics
Lactobacillus rhamnosus
Bifidobacterium longum
Streptococcus mutans
Lactobacillus spp
Caries prevention
Early childhood caries
Clinical trial
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100454&lng=en&tlng=en
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