Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial
Abstract Probiotics have provided benefits to general health, but they are still insufficient to dental health. Objective: This study aimed to evaluate milk supplemented with probiotic bacteria and standard milk, measured by levels of Streptococcus mutans (S. mutans) and Lactobacillus spp., in 3-4-...
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doaj-1ebc15044faf40ec8ab11197441bdbe92020-11-24T23:48:08ZengUniversity of São PauloJournal of Applied Oral Science1678-77652018-05-0126010.1590/1678-7757-2017-0318S1678-77572018000100454Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trialJudy VillavicencioLina Maria VillegasMaria Cristina ArangoSusana AriasFrancia TrianaAbstract Probiotics have provided benefits to general health, but they are still insufficient to dental health. Objective: This study aimed to evaluate milk supplemented with probiotic bacteria and standard milk, measured by levels of Streptococcus mutans (S. mutans) and Lactobacillus spp., in 3-4-year-old children after 9 months of intervention. Material and Methods: The study was a triple-blind, placebo-controlled, randomized trial. The sample was composed of 363 preschoolers attending five child development centers in Cali, Colombia. They were randomized to two groups: children in the intervention group drank 200 mL of milk with Lactobacillus rhamnosus 5x106 and Bifidobacteruim longum 3x106, and children in the control group drank 200 mL of standard milk. Interventions occurred on weekdays and information was gathered through scheduled clinical examination. The primary result was the number of colony forming units (CFU) of S. mutans and Lactobacillus spp. in the saliva. Secondary results were dental caries, rated by the International Caries Detection and Assessment System (ICDAS), dental plaque, pH, and salivary buffer capacity. Results: The proportion of S. mutans was lower in the intervention group compared with the control group after 9 months; however, the differences did not reach statistical significance (p=0.173); on the other hand, statistically significant differences between groups were found in the CFU/mL of Lactobacillus spp. (p=0.002). There was not statistically significant difference in the prevalence of dental caries for both groups (p=0.767). Differences between groups were found in the salivary buffering capacity (p=0.000); neither salivary pH nor dental plaque were significantly different. Conclusions: Regular consumption of milk containing probiotics bacteria reduced CFU/mL of Lactobacillus spp. and increased salivary buffering capacity at 9 months of consumption.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100454&lng=en&tlng=enPreschool childrenProbioticsLactobacillus rhamnosusBifidobacterium longumStreptococcus mutansLactobacillus sppCaries preventionEarly childhood cariesClinical trial |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Judy Villavicencio Lina Maria Villegas Maria Cristina Arango Susana Arias Francia Triana |
spellingShingle |
Judy Villavicencio Lina Maria Villegas Maria Cristina Arango Susana Arias Francia Triana Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial Journal of Applied Oral Science Preschool children Probiotics Lactobacillus rhamnosus Bifidobacterium longum Streptococcus mutans Lactobacillus spp Caries prevention Early childhood caries Clinical trial |
author_facet |
Judy Villavicencio Lina Maria Villegas Maria Cristina Arango Susana Arias Francia Triana |
author_sort |
Judy Villavicencio |
title |
Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial |
title_short |
Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial |
title_full |
Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial |
title_fullStr |
Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial |
title_full_unstemmed |
Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial |
title_sort |
effects of a food enriched with probiotics on streptococcus mutans and lactobacillus spp. salivary counts in preschool children: a cluster randomized trial |
publisher |
University of São Paulo |
series |
Journal of Applied Oral Science |
issn |
1678-7765 |
publishDate |
2018-05-01 |
description |
Abstract Probiotics have provided benefits to general health, but they are still insufficient to dental health. Objective: This study aimed to evaluate milk supplemented with probiotic bacteria and standard milk, measured by levels of Streptococcus mutans (S. mutans) and Lactobacillus spp., in 3-4-year-old children after 9 months of intervention. Material and Methods: The study was a triple-blind, placebo-controlled, randomized trial. The sample was composed of 363 preschoolers attending five child development centers in Cali, Colombia. They were randomized to two groups: children in the intervention group drank 200 mL of milk with Lactobacillus rhamnosus 5x106 and Bifidobacteruim longum 3x106, and children in the control group drank 200 mL of standard milk. Interventions occurred on weekdays and information was gathered through scheduled clinical examination. The primary result was the number of colony forming units (CFU) of S. mutans and Lactobacillus spp. in the saliva. Secondary results were dental caries, rated by the International Caries Detection and Assessment System (ICDAS), dental plaque, pH, and salivary buffer capacity. Results: The proportion of S. mutans was lower in the intervention group compared with the control group after 9 months; however, the differences did not reach statistical significance (p=0.173); on the other hand, statistically significant differences between groups were found in the CFU/mL of Lactobacillus spp. (p=0.002). There was not statistically significant difference in the prevalence of dental caries for both groups (p=0.767). Differences between groups were found in the salivary buffering capacity (p=0.000); neither salivary pH nor dental plaque were significantly different. Conclusions: Regular consumption of milk containing probiotics bacteria reduced CFU/mL of Lactobacillus spp. and increased salivary buffering capacity at 9 months of consumption. |
topic |
Preschool children Probiotics Lactobacillus rhamnosus Bifidobacterium longum Streptococcus mutans Lactobacillus spp Caries prevention Early childhood caries Clinical trial |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100454&lng=en&tlng=en |
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