Coenzyme Q10 Protects Hippocampal Neurons against Ischemia/ Reperfusion Injury via Modulation of BAX/Bcl-2 Expression

Introduction: Preliminary studies have con.rmed reduction in cell death following treatment with antioxidants. According to this .nding we study the relationship between consumption of CoQ10 and expression of Bax and Bcl2 in hippocampus following ischemia/reperfusion as proteins involved in cell pro...

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Main Authors: Mohammad Zamani, Majid Katebi, Mehdi Mehdizadeh, Farzaneh Mohamadzadeh, Mansooreh Soleimani
Format: Article
Language:English
Published: Iran University of Medical Sciences 2012-09-01
Series:Basic and Clinical Neuroscience
Subjects:
Online Access:http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-125&slc_lang=en&sid=1
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spelling doaj-1f9ef90339504b76a6157a9b68c201382020-11-24T22:23:56ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422012-09-0135510Coenzyme Q10 Protects Hippocampal Neurons against Ischemia/ Reperfusion Injury via Modulation of BAX/Bcl-2 ExpressionMohammad Zamani0Majid Katebi1Mehdi Mehdizadeh2Farzaneh Mohamadzadeh3Mansooreh Soleimani4 Introduction: Preliminary studies have con.rmed reduction in cell death following treatment with antioxidants. According to this .nding we study the relationship between consumption of CoQ10 and expression of Bax and Bcl2 in hippocampus following ischemia/reperfusion as proteins involved in cell programmed death or apoptosis. Methods: We studied the protective role of CoQ10 against ischemia-reperfusion. Experimental design includes four groups:  intact, ischemic control, sham control and treatment group with CoQ10. The mice were pre-treated with CoQ10 for a week, then ischemia was induced by common carotid artery ligation and following the reduction in in.ammation (a week) the mice was treated with CoQ10.  Nissl staining was applied for counting the necrotic cells of hippocampus and the western blot was performed to measure the Bax and Bcl2 expression.Results: Cell death was signi.cantly lower when mice were treated with CoQ10. Bax expression was signi.cantly high in the ischemic group but low in the treatment group, and the bcl2 expression was lower in the ischemic group than the treatment and the vehicle groups.Discussion: Ischemia for 15 minutes induced cell death in hippocampus with more potent effect on CA1. CoQ10 intake signi.cantly reduced cell death and prevented the expression of Bax while inducing an increase in expression of bcl2.http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-125&slc_lang=en&sid=1CoQ10 Hippocampus Ischemia-Reperfusion Neuroprotective Effect.
collection DOAJ
language English
format Article
sources DOAJ
author Mohammad Zamani
Majid Katebi
Mehdi Mehdizadeh
Farzaneh Mohamadzadeh
Mansooreh Soleimani
spellingShingle Mohammad Zamani
Majid Katebi
Mehdi Mehdizadeh
Farzaneh Mohamadzadeh
Mansooreh Soleimani
Coenzyme Q10 Protects Hippocampal Neurons against Ischemia/ Reperfusion Injury via Modulation of BAX/Bcl-2 Expression
Basic and Clinical Neuroscience
CoQ10
Hippocampus
Ischemia-Reperfusion
Neuroprotective Effect.
author_facet Mohammad Zamani
Majid Katebi
Mehdi Mehdizadeh
Farzaneh Mohamadzadeh
Mansooreh Soleimani
author_sort Mohammad Zamani
title Coenzyme Q10 Protects Hippocampal Neurons against Ischemia/ Reperfusion Injury via Modulation of BAX/Bcl-2 Expression
title_short Coenzyme Q10 Protects Hippocampal Neurons against Ischemia/ Reperfusion Injury via Modulation of BAX/Bcl-2 Expression
title_full Coenzyme Q10 Protects Hippocampal Neurons against Ischemia/ Reperfusion Injury via Modulation of BAX/Bcl-2 Expression
title_fullStr Coenzyme Q10 Protects Hippocampal Neurons against Ischemia/ Reperfusion Injury via Modulation of BAX/Bcl-2 Expression
title_full_unstemmed Coenzyme Q10 Protects Hippocampal Neurons against Ischemia/ Reperfusion Injury via Modulation of BAX/Bcl-2 Expression
title_sort coenzyme q10 protects hippocampal neurons against ischemia/ reperfusion injury via modulation of bax/bcl-2 expression
publisher Iran University of Medical Sciences
series Basic and Clinical Neuroscience
issn 2008-126X
2228-7442
publishDate 2012-09-01
description Introduction: Preliminary studies have con.rmed reduction in cell death following treatment with antioxidants. According to this .nding we study the relationship between consumption of CoQ10 and expression of Bax and Bcl2 in hippocampus following ischemia/reperfusion as proteins involved in cell programmed death or apoptosis. Methods: We studied the protective role of CoQ10 against ischemia-reperfusion. Experimental design includes four groups:  intact, ischemic control, sham control and treatment group with CoQ10. The mice were pre-treated with CoQ10 for a week, then ischemia was induced by common carotid artery ligation and following the reduction in in.ammation (a week) the mice was treated with CoQ10.  Nissl staining was applied for counting the necrotic cells of hippocampus and the western blot was performed to measure the Bax and Bcl2 expression.Results: Cell death was signi.cantly lower when mice were treated with CoQ10. Bax expression was signi.cantly high in the ischemic group but low in the treatment group, and the bcl2 expression was lower in the ischemic group than the treatment and the vehicle groups.Discussion: Ischemia for 15 minutes induced cell death in hippocampus with more potent effect on CA1. CoQ10 intake signi.cantly reduced cell death and prevented the expression of Bax while inducing an increase in expression of bcl2.
topic CoQ10
Hippocampus
Ischemia-Reperfusion
Neuroprotective Effect.
url http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-125&slc_lang=en&sid=1
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