Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure

Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure

The hydroxylation of n-alkanes, which proceeds in the presence of a P450-monooxygenase advantageously at temperatures significantly below room temperature, is described. In addition, an enzymatic hydroxylation of the “liquid gas” n-butane with in situ cofactor regeneration, which does not require hi...

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Main Authors: Svenja Staudt, Christina A. Müller, Jan Marienhagen, Christian Böing, Stefan Buchholz, Ulrich Schwaneberg, Harald Gröger
Format: Article
Language:English
Published: Beilstein-Institut 2012-02-01
Series:Beilstein Journal of Organic Chemistry
Subjects:
biotransformations
cofactor regeneration
green chemistry
hydroxylation
P450-monooxygenase
Online Access:https://doi.org/10.3762/bjoc.8.20
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spelling doaj-1fad91934f3b4507bf388879cd46819e2021-02-02T00:50:50ZengBeilstein-InstitutBeilstein Journal of Organic Chemistry1860-53972012-02-018118619110.3762/bjoc.8.201860-5397-8-20Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressureSvenja Staudt0Christina A. Müller1Jan Marienhagen2Christian Böing3Stefan Buchholz4Ulrich Schwaneberg5Harald Gröger6Department of Chemistry and Pharmacy, University of Erlangen-Nürnberg, Henkestr. 42, 91054 Erlangen, GermanyDepartment of Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, GermanyDepartment of Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, GermanyEvonik Oxeno GmbH, Paul-Baumann-Straße 1, 45772 Marl, GermanyEvonik Degussa GmbH, Industrial Chemicals, Paul-Baumann-Straße 1, 45772 Marl, GermanyDepartment of Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, GermanyDepartment of Chemistry and Pharmacy, University of Erlangen-Nürnberg, Henkestr. 42, 91054 Erlangen, GermanyThe hydroxylation of n-alkanes, which proceeds in the presence of a P450-monooxygenase advantageously at temperatures significantly below room temperature, is described. In addition, an enzymatic hydroxylation of the “liquid gas” n-butane with in situ cofactor regeneration, which does not require high-pressure conditions, was developed. The resulting 2-butanol was obtained as the only regioisomer, at a product concentration of 0.16 g/L.https://doi.org/10.3762/bjoc.8.20biotransformationscofactor regenerationgreen chemistryhydroxylationP450-monooxygenase
collection DOAJ
language English
format Article
sources DOAJ
author Svenja Staudt
Christina A. Müller
Jan Marienhagen
Christian Böing
Stefan Buchholz
Ulrich Schwaneberg
Harald Gröger
spellingShingle Svenja Staudt
Christina A. Müller
Jan Marienhagen
Christian Böing
Stefan Buchholz
Ulrich Schwaneberg
Harald Gröger
Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
Beilstein Journal of Organic Chemistry
biotransformations
cofactor regeneration
green chemistry
hydroxylation
P450-monooxygenase
author_facet Svenja Staudt
Christina A. Müller
Jan Marienhagen
Christian Böing
Stefan Buchholz
Ulrich Schwaneberg
Harald Gröger
author_sort Svenja Staudt
title Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_short Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_full Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_fullStr Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_full_unstemmed Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
title_sort biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure
publisher Beilstein-Institut
series Beilstein Journal of Organic Chemistry
issn 1860-5397
publishDate 2012-02-01
description The hydroxylation of n-alkanes, which proceeds in the presence of a P450-monooxygenase advantageously at temperatures significantly below room temperature, is described. In addition, an enzymatic hydroxylation of the “liquid gas” n-butane with in situ cofactor regeneration, which does not require high-pressure conditions, was developed. The resulting 2-butanol was obtained as the only regioisomer, at a product concentration of 0.16 g/L.
topic biotransformations
cofactor regeneration
green chemistry
hydroxylation
P450-monooxygenase
url https://doi.org/10.3762/bjoc.8.20
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AT christinaamuller biocatalytichydroxylationofnbutanewithinsitucofactorregenerationatlowtemperatureandundernormalpressure
AT janmarienhagen biocatalytichydroxylationofnbutanewithinsitucofactorregenerationatlowtemperatureandundernormalpressure
AT christianboing biocatalytichydroxylationofnbutanewithinsitucofactorregenerationatlowtemperatureandundernormalpressure
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AT ulrichschwaneberg biocatalytichydroxylationofnbutanewithinsitucofactorregenerationatlowtemperatureandundernormalpressure
AT haraldgroger biocatalytichydroxylationofnbutanewithinsitucofactorregenerationatlowtemperatureandundernormalpressure
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