Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco
Abstract Background In recent decades, there has been a marked increase in the number of reported cases of pertussis around the world, and pertussis continues to be a frequently occurring disease despite an effective childhood vaccination. This study aims to determine the role of household contacts...
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doaj-21cf7789fe0e49d2ba138f45a0583a702021-01-17T12:08:22ZengBMCBMC Infectious Diseases1471-23342020-01-012011910.1186/s12879-019-4680-1Pertussis in infants, in their mothers and other contacts in Casablanca, MoroccoKhalid Katfy0Idrissa Diawara1Fakhredine Maaloum2Siham Aziz3Nicole Guiso4Hassan Fellah5Bouchra Slaoui6Khalid Zerouali7Houria Belabbes8Naima Elmdaghri9Department of Microbiology, Faculty of Medicine and PharmacyDepartment of Microbiology, Faculty of Medicine and PharmacyDepartment of Microbiology, Faculty of Medicine and PharmacyBacteriology-Virology and Hospital Hygiene Laboratory, University Hospital Centre Ibn RochdMolecular Prevention and Therapy of Human Diseases, Institut PasteurDepartment of Immunology, Faculty of Medicine and PharmacyAbderrahim Harouchi Pediatric HospitalDepartment of Microbiology, Faculty of Medicine and PharmacyDepartment of Microbiology, Faculty of Medicine and PharmacyDepartment of Microbiology, Faculty of Medicine and PharmacyAbstract Background In recent decades, there has been a marked increase in the number of reported cases of pertussis around the world, and pertussis continues to be a frequently occurring disease despite an effective childhood vaccination. This study aims to determine the role of household contacts of children diagnosed with pertussis in Casablanca Morocco. Methods From November 2015 to October 2017, children suspected of whooping cough that consulted Ibn Rochd University hospital at Casablanca with their household contacts were enrolled in the study. Nasopharyngeal (NP) samples of the suspected children were analyzed by culture and RT-PCR. For the household contacts, NP and blood samples were collected and analyzed by RT-PCR and specific detection of pertussis toxin antibodies by ELISA, respectively. Results During the study period, the survey was carried out on 128 infants hospitalized for pertussis suspicion and their families (N = 140). B. pertussis DNA was specifically detected in 73 (57%) samples, coexistence of B. pertussis and B. parapertussis DNA in 3 (2.3%) samples, coexistence of B. pertussis and B. holmesii DNA in 10 (7.81%) and only one (0.78%) sample was IS 481 RT-PCR positive without the possibility of determining the Bordetella species with the diagnostic tools used. Confirmations of Pertussis infection in household contacts by culture, RT- PCR and serology were 10, 46 and 39%, respectively. B. pertussis DNA was confirmed in the infants as well in their mothers in 38% of the cases. Co detection of B. pertussis and B. parapertussis DNA in 2% and co-detection of B. pertussis and B. holmesii DNA in 4%. B. holmesii DNA alone was detected in 5 NP samples of index cases and their mothers. Conclusions The results of this study confirm that B. pertussis is still circulating in children and adults, and were likely a source of pertussis contamination in infants still not vaccinated. The use of RT-PCR specific for B. pertussis in the diagnosis of adults is less sensitive and should be associated with serologic tests to improve diagnosis of pertussis and contributes to preventing transmission of the disease in infants.https://doi.org/10.1186/s12879-019-4680-1PertussisB. holmesiiB. ParapertussisHousehold contactsAnti-PT IgG/IgA |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Khalid Katfy Idrissa Diawara Fakhredine Maaloum Siham Aziz Nicole Guiso Hassan Fellah Bouchra Slaoui Khalid Zerouali Houria Belabbes Naima Elmdaghri |
spellingShingle |
Khalid Katfy Idrissa Diawara Fakhredine Maaloum Siham Aziz Nicole Guiso Hassan Fellah Bouchra Slaoui Khalid Zerouali Houria Belabbes Naima Elmdaghri Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco BMC Infectious Diseases Pertussis B. holmesii B. Parapertussis Household contacts Anti-PT IgG/IgA |
author_facet |
Khalid Katfy Idrissa Diawara Fakhredine Maaloum Siham Aziz Nicole Guiso Hassan Fellah Bouchra Slaoui Khalid Zerouali Houria Belabbes Naima Elmdaghri |
author_sort |
Khalid Katfy |
title |
Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco |
title_short |
Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco |
title_full |
Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco |
title_fullStr |
Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco |
title_full_unstemmed |
Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco |
title_sort |
pertussis in infants, in their mothers and other contacts in casablanca, morocco |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2020-01-01 |
description |
Abstract Background In recent decades, there has been a marked increase in the number of reported cases of pertussis around the world, and pertussis continues to be a frequently occurring disease despite an effective childhood vaccination. This study aims to determine the role of household contacts of children diagnosed with pertussis in Casablanca Morocco. Methods From November 2015 to October 2017, children suspected of whooping cough that consulted Ibn Rochd University hospital at Casablanca with their household contacts were enrolled in the study. Nasopharyngeal (NP) samples of the suspected children were analyzed by culture and RT-PCR. For the household contacts, NP and blood samples were collected and analyzed by RT-PCR and specific detection of pertussis toxin antibodies by ELISA, respectively. Results During the study period, the survey was carried out on 128 infants hospitalized for pertussis suspicion and their families (N = 140). B. pertussis DNA was specifically detected in 73 (57%) samples, coexistence of B. pertussis and B. parapertussis DNA in 3 (2.3%) samples, coexistence of B. pertussis and B. holmesii DNA in 10 (7.81%) and only one (0.78%) sample was IS 481 RT-PCR positive without the possibility of determining the Bordetella species with the diagnostic tools used. Confirmations of Pertussis infection in household contacts by culture, RT- PCR and serology were 10, 46 and 39%, respectively. B. pertussis DNA was confirmed in the infants as well in their mothers in 38% of the cases. Co detection of B. pertussis and B. parapertussis DNA in 2% and co-detection of B. pertussis and B. holmesii DNA in 4%. B. holmesii DNA alone was detected in 5 NP samples of index cases and their mothers. Conclusions The results of this study confirm that B. pertussis is still circulating in children and adults, and were likely a source of pertussis contamination in infants still not vaccinated. The use of RT-PCR specific for B. pertussis in the diagnosis of adults is less sensitive and should be associated with serologic tests to improve diagnosis of pertussis and contributes to preventing transmission of the disease in infants. |
topic |
Pertussis B. holmesii B. Parapertussis Household contacts Anti-PT IgG/IgA |
url |
https://doi.org/10.1186/s12879-019-4680-1 |
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