Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by <i>Staphylococcus</i> <i>aureus</i> and <i>Pseudomonas aeruginosa</i>

The formation of biofilms on biomaterials causes biofilm-associated infections. Available treatments often fail to fight the microorganisms in the biofilm, creating serious risks for patient well-being and life. Due to their significant antibiofilm activities, antimicrobial peptides are being intens...

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Main Authors: Malgorzata Anna Paduszynska, Katarzyna Ewa Greber, Wojciech Paduszynski, Wieslaw Sawicki, Wojciech Kamysz
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Antibiotics
Subjects:
Online Access:https://www.mdpi.com/2079-6382/9/9/566
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spelling doaj-22a268a59bfc47fdad65fbc31100c4cd2020-11-25T01:25:10ZengMDPI AGAntibiotics2079-63822020-09-01956656610.3390/antibiotics9090566Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by <i>Staphylococcus</i> <i>aureus</i> and <i>Pseudomonas aeruginosa</i>Malgorzata Anna Paduszynska0Katarzyna Ewa Greber1Wojciech Paduszynski2Wieslaw Sawicki3Wojciech Kamysz4Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdańsk, PolandDepartment of Physical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdańsk, PolandDepartment of Surgery, Poviat Hospital in Bytow, 77-100 Bytow, PolandDepartment of Physical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdańsk, PolandDepartment of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdańsk, PolandThe formation of biofilms on biomaterials causes biofilm-associated infections. Available treatments often fail to fight the microorganisms in the biofilm, creating serious risks for patient well-being and life. Due to their significant antibiofilm activities, antimicrobial peptides are being intensively investigated in this regard. A promising approach is a combination therapy that aims to increase the efficacy and broaden the spectrum of antibiotics. The main goal of this study was to evaluate the antimicrobial efficacy of temporin A and the short lipopeptides (C10)2-KKKK-NH2 and (C12)2-KKKK-NH2 in combination with gentamicin against biofilm formed by Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA). Peptides were synthesized with solid-phase temperature-assisted synthesis methodology. The minimum inhibitory concentrations (MICs), fractional inhibitory concentrations (FICs), minimum biofilm eradication concentrations (MBECs), and the influence of combinations of compounds with gentamicin on bacterial biofilm were determined for reference strains of SA (ATCC 25923) and PA (ATCC 9027). The peptides exhibited significant potential to enhance the antibacterial activity of gentamicin against SA biofilm, but there was no synergy in activity against planktonic cells. The antibiotic applied alone demonstrated strong activity against planktonic cells and poor effectiveness against SA biofilm. Biofilm formed by PA was much more sensitive to gentamicin, but some positive influences of supplementation with peptides were noticed. The results of the performed experiments suggest that the potential application of peptides as adjuvant agents in the treatment of biofilm-associated infections should be studied further.https://www.mdpi.com/2079-6382/9/9/566lipopeptidesantimicrobial peptidesgentamicinbiofilm-associated infectionsmicrobial resistancecombination therapy
collection DOAJ
language English
format Article
sources DOAJ
author Malgorzata Anna Paduszynska
Katarzyna Ewa Greber
Wojciech Paduszynski
Wieslaw Sawicki
Wojciech Kamysz
spellingShingle Malgorzata Anna Paduszynska
Katarzyna Ewa Greber
Wojciech Paduszynski
Wieslaw Sawicki
Wojciech Kamysz
Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by <i>Staphylococcus</i> <i>aureus</i> and <i>Pseudomonas aeruginosa</i>
Antibiotics
lipopeptides
antimicrobial peptides
gentamicin
biofilm-associated infections
microbial resistance
combination therapy
author_facet Malgorzata Anna Paduszynska
Katarzyna Ewa Greber
Wojciech Paduszynski
Wieslaw Sawicki
Wojciech Kamysz
author_sort Malgorzata Anna Paduszynska
title Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by <i>Staphylococcus</i> <i>aureus</i> and <i>Pseudomonas aeruginosa</i>
title_short Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by <i>Staphylococcus</i> <i>aureus</i> and <i>Pseudomonas aeruginosa</i>
title_full Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by <i>Staphylococcus</i> <i>aureus</i> and <i>Pseudomonas aeruginosa</i>
title_fullStr Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by <i>Staphylococcus</i> <i>aureus</i> and <i>Pseudomonas aeruginosa</i>
title_full_unstemmed Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by <i>Staphylococcus</i> <i>aureus</i> and <i>Pseudomonas aeruginosa</i>
title_sort activity of temporin a and short lipopeptides combined with gentamicin against biofilm formed by <i>staphylococcus</i> <i>aureus</i> and <i>pseudomonas aeruginosa</i>
publisher MDPI AG
series Antibiotics
issn 2079-6382
publishDate 2020-09-01
description The formation of biofilms on biomaterials causes biofilm-associated infections. Available treatments often fail to fight the microorganisms in the biofilm, creating serious risks for patient well-being and life. Due to their significant antibiofilm activities, antimicrobial peptides are being intensively investigated in this regard. A promising approach is a combination therapy that aims to increase the efficacy and broaden the spectrum of antibiotics. The main goal of this study was to evaluate the antimicrobial efficacy of temporin A and the short lipopeptides (C10)2-KKKK-NH2 and (C12)2-KKKK-NH2 in combination with gentamicin against biofilm formed by Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA). Peptides were synthesized with solid-phase temperature-assisted synthesis methodology. The minimum inhibitory concentrations (MICs), fractional inhibitory concentrations (FICs), minimum biofilm eradication concentrations (MBECs), and the influence of combinations of compounds with gentamicin on bacterial biofilm were determined for reference strains of SA (ATCC 25923) and PA (ATCC 9027). The peptides exhibited significant potential to enhance the antibacterial activity of gentamicin against SA biofilm, but there was no synergy in activity against planktonic cells. The antibiotic applied alone demonstrated strong activity against planktonic cells and poor effectiveness against SA biofilm. Biofilm formed by PA was much more sensitive to gentamicin, but some positive influences of supplementation with peptides were noticed. The results of the performed experiments suggest that the potential application of peptides as adjuvant agents in the treatment of biofilm-associated infections should be studied further.
topic lipopeptides
antimicrobial peptides
gentamicin
biofilm-associated infections
microbial resistance
combination therapy
url https://www.mdpi.com/2079-6382/9/9/566
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