Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras
<p class="DefaultCxSpFirst">Gelatin as the main ingredient of capsules is still a problem for a moslem. Most of gelatin production remains largely derived from non-halal materials. One of gelatin source is came from collagen of the skin and bones of bovine or pork. The main of study...
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Syarif Hidayatullah Jakarta State Islamic University
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doaj-23500ea9a74741968929d70e6b86c0b32020-11-24T22:35:51ZindSyarif Hidayatullah Jakarta State Islamic UniversityJurnal Kimia Valensi2460-60652548-30132016-08-0100263210.15408/jkv.v0i0.31502727Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul KerasSandra Hermanto0Fahrur Rahman Saputra1Zilhadia .2Program Studi Kimia Fakultas Sains dan Teknologi Universitas Islam Negeri Syarif Hidayatullah JakartaPragram Studi Farmasi, FKIK UIN Syarif Hidayatullah JakartaPragram Studi Farmasi, FKIK UIN Syarif Hidayatullah Jakarta<p class="DefaultCxSpFirst">Gelatin as the main ingredient of capsules is still a problem for a moslem. Most of gelatin production remains largely derived from non-halal materials. One of gelatin source is came from collagen of the skin and bones of bovine or pork. The main of study is determine the source of gelatin used in hard capsules by using SDS-PAGE (<em>Sodium Dodecyl Sulphate Gel electrophoresis Poly Acrylamide</em>) method. In the early stages, optimization of standards bovine and pork gelatin were hydrolyzed by pepsin at pH 4.5 and 60°C for 1 hour, 2 hours, and 3 hours. Gelatin hydrolyzateswere analyzed by SDS-PAGE to determine the optimal hydrolysis time. Identification of gelatin hydrolyzate fragments were carried by molecular weight. Hydrolysis time optimization throught applied to identify the source of hard gelatin capsules in the samples obtained from market and compared with the simulation of hard gelatin capsules. The results showed there were of specific bands of bovine gelatin with a molecular weight of 11,4 kDa; 34 kDa; 47kDa and specific bands of pork gelatin with a molecular weight of 24.7 kDa; 28 kDa; and 60 kDa. Similar results were obtained on a sample of hard capsules with bands of protein fragments that were identical to bovine gelatinstandard. Based on the results,each of the samples were tested contain of bovine gelatin respectively.</p> <p class="DefaultCxSpLast"><strong><em>DOI :</em></strong><a href="http://dx.doi.org/10.15408/jkv.v0i0.3150"><em>http://dx.doi.org/10.15408/jkv.v0i0.3150</em></a></p>http://journal.uinjkt.ac.id/index.php/valensi/article/view/3150gelatinhydrolysishard capsulespepsinSDS PAGE |
collection |
DOAJ |
language |
Indonesian |
format |
Article |
sources |
DOAJ |
author |
Sandra Hermanto Fahrur Rahman Saputra Zilhadia . |
spellingShingle |
Sandra Hermanto Fahrur Rahman Saputra Zilhadia . Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras Jurnal Kimia Valensi gelatin hydrolysis hard capsules pepsin SDS PAGE |
author_facet |
Sandra Hermanto Fahrur Rahman Saputra Zilhadia . |
author_sort |
Sandra Hermanto |
title |
Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras |
title_short |
Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras |
title_full |
Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras |
title_fullStr |
Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras |
title_full_unstemmed |
Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras |
title_sort |
aplikasi metode sds-page (sodium dodecyl sulphate poly acrylamide gel electrophoresis) untuk mengidentifikasi sumber asal gelatin pada kapsul keras |
publisher |
Syarif Hidayatullah Jakarta State Islamic University |
series |
Jurnal Kimia Valensi |
issn |
2460-6065 2548-3013 |
publishDate |
2016-08-01 |
description |
<p class="DefaultCxSpFirst">Gelatin as the main ingredient of capsules is still a problem for a moslem. Most of gelatin production remains largely derived from non-halal materials. One of gelatin source is came from collagen of the skin and bones of bovine or pork. The main of study is determine the source of gelatin used in hard capsules by using SDS-PAGE (<em>Sodium Dodecyl Sulphate Gel electrophoresis Poly Acrylamide</em>) method. In the early stages, optimization of standards bovine and pork gelatin were hydrolyzed by pepsin at pH 4.5 and 60°C for 1 hour, 2 hours, and 3 hours. Gelatin hydrolyzateswere analyzed by SDS-PAGE to determine the optimal hydrolysis time. Identification of gelatin hydrolyzate fragments were carried by molecular weight. Hydrolysis time optimization throught applied to identify the source of hard gelatin capsules in the samples obtained from market and compared with the simulation of hard gelatin capsules. The results showed there were of specific bands of bovine gelatin with a molecular weight of 11,4 kDa; 34 kDa; 47kDa and specific bands of pork gelatin with a molecular weight of 24.7 kDa; 28 kDa; and 60 kDa. Similar results were obtained on a sample of hard capsules with bands of protein fragments that were identical to bovine gelatinstandard. Based on the results,each of the samples were tested contain of bovine gelatin respectively.</p> <p class="DefaultCxSpLast"><strong><em>DOI :</em></strong><a href="http://dx.doi.org/10.15408/jkv.v0i0.3150"><em>http://dx.doi.org/10.15408/jkv.v0i0.3150</em></a></p> |
topic |
gelatin hydrolysis hard capsules pepsin SDS PAGE |
url |
http://journal.uinjkt.ac.id/index.php/valensi/article/view/3150 |
work_keys_str_mv |
AT sandrahermanto aplikasimetodesdspagesodiumdodecylsulphatepolyacrylamidegelelectrophoresisuntukmengidentifikasisumberasalgelatinpadakapsulkeras AT fahrurrahmansaputra aplikasimetodesdspagesodiumdodecylsulphatepolyacrylamidegelelectrophoresisuntukmengidentifikasisumberasalgelatinpadakapsulkeras AT zilhadia aplikasimetodesdspagesodiumdodecylsulphatepolyacrylamidegelelectrophoresisuntukmengidentifikasisumberasalgelatinpadakapsulkeras |
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