Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue Engineering
Thermosensitive, physically crosslinked injectable hydrogels are in the area of interests of various scientific fields. One of the representatives of this materials group is an aqueous solution of methylcellulose. At ambient conditions, methylcellulose (MC) is a sol while on heating up to 37 ...
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doaj-253092b10ed740ee9a00c1509316ceb62020-11-25T02:22:04ZengMDPI AGPolymers2073-43602019-10-011111177210.3390/polym11111772polym11111772Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue EngineeringBeata Niemczyk-Soczynska0Arkadiusz Gradys1Dorota Kolbuk2Anna Krzton-Maziopa3Pawel Sajkiewicz4Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawinskiego 5b St., 02-106 Warsaw, PolandInstitute of Fundamental Technological Research, Polish Academy of Sciences, Pawinskiego 5b St., 02-106 Warsaw, PolandInstitute of Fundamental Technological Research, Polish Academy of Sciences, Pawinskiego 5b St., 02-106 Warsaw, PolandFaculty of Chemistry, Warsaw University of Technology, Noakowskiego 3 St., 00-664 Warsaw, PolandInstitute of Fundamental Technological Research, Polish Academy of Sciences, Pawinskiego 5b St., 02-106 Warsaw, PolandThermosensitive, physically crosslinked injectable hydrogels are in the area of interests of various scientific fields. One of the representatives of this materials group is an aqueous solution of methylcellulose. At ambient conditions, methylcellulose (MC) is a sol while on heating up to 37 °C, MC undergoes physical crosslinking and transforms into a gel. Injectability at room temperature, and crosslinkability during subsequent heating to physiological temperature raises hopes, especially for tissue engineering applications. This research work aimed at studying crosslinking kinetics, thermal, viscoelastic, and biological properties of MC aqueous solution in a broad range of MC concentrations. It was evidenced by Differential Scanning Calorimetry (DSC) that crosslinking of MC is a reversible two-stage process, manifested by the appearance of two endothermic effects, related to the destruction of water cages around methoxy groups, followed by crosslinking via the formation of hydrophobic interactions between methoxy groups in the polymeric chains. The DSC results also allowed the determination of MC crosslinking kinetics. Complementary measurements of MC crosslinking kinetics performed by dynamic mechanical analysis (DMA) provided information on the final storage modulus, which was important from the perspective of tissue engineering applications. Cytotoxicity tests were performed using mouse fibroblasts and showed that MC at low concentration did not cause cytotoxicity. All these efforts allowed to assess MC hydrogel relevance for tissue engineering applications.https://www.mdpi.com/2073-4360/11/11/1772methylcellulosethermosensitive hydrogelcrosslinking kineticsdscdmacellular tests |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Beata Niemczyk-Soczynska Arkadiusz Gradys Dorota Kolbuk Anna Krzton-Maziopa Pawel Sajkiewicz |
spellingShingle |
Beata Niemczyk-Soczynska Arkadiusz Gradys Dorota Kolbuk Anna Krzton-Maziopa Pawel Sajkiewicz Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue Engineering Polymers methylcellulose thermosensitive hydrogel crosslinking kinetics dsc dma cellular tests |
author_facet |
Beata Niemczyk-Soczynska Arkadiusz Gradys Dorota Kolbuk Anna Krzton-Maziopa Pawel Sajkiewicz |
author_sort |
Beata Niemczyk-Soczynska |
title |
Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue Engineering |
title_short |
Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue Engineering |
title_full |
Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue Engineering |
title_fullStr |
Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue Engineering |
title_full_unstemmed |
Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue Engineering |
title_sort |
crosslinking kinetics of methylcellulose aqueous solution and its potential as a scaffold for tissue engineering |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2019-10-01 |
description |
Thermosensitive, physically crosslinked injectable hydrogels are in the area of interests of various scientific fields. One of the representatives of this materials group is an aqueous solution of methylcellulose. At ambient conditions, methylcellulose (MC) is a sol while on heating up to 37 °C, MC undergoes physical crosslinking and transforms into a gel. Injectability at room temperature, and crosslinkability during subsequent heating to physiological temperature raises hopes, especially for tissue engineering applications. This research work aimed at studying crosslinking kinetics, thermal, viscoelastic, and biological properties of MC aqueous solution in a broad range of MC concentrations. It was evidenced by Differential Scanning Calorimetry (DSC) that crosslinking of MC is a reversible two-stage process, manifested by the appearance of two endothermic effects, related to the destruction of water cages around methoxy groups, followed by crosslinking via the formation of hydrophobic interactions between methoxy groups in the polymeric chains. The DSC results also allowed the determination of MC crosslinking kinetics. Complementary measurements of MC crosslinking kinetics performed by dynamic mechanical analysis (DMA) provided information on the final storage modulus, which was important from the perspective of tissue engineering applications. Cytotoxicity tests were performed using mouse fibroblasts and showed that MC at low concentration did not cause cytotoxicity. All these efforts allowed to assess MC hydrogel relevance for tissue engineering applications. |
topic |
methylcellulose thermosensitive hydrogel crosslinking kinetics dsc dma cellular tests |
url |
https://www.mdpi.com/2073-4360/11/11/1772 |
work_keys_str_mv |
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1724863621468520448 |