Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β Cells
Background: Increasing evidence indicates that long noncoding RNAs (IncRNAs) perform specific biological functions in diverse processes. Recent studies have reported that IncRNAs may be involved in β cell function. The aim of this study was to characterize the role of IncRNA TUG1 in mouse pancreatic...
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Cell Physiol Biochem Press GmbH & Co KG
2015-03-01
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doaj-27b9fd601c8c492d813b5c22485cd08d2020-11-25T02:15:33ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-03-013551892190410.1159/000373999373999Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β CellsDan-dan YinEr-bao ZhangLiang-hui YouNing WangLin-tao WangFei-yan JinYa-nan ZhuLi-hua CaoQing-xin YuanWei DeWei TangBackground: Increasing evidence indicates that long noncoding RNAs (IncRNAs) perform specific biological functions in diverse processes. Recent studies have reported that IncRNAs may be involved in β cell function. The aim of this study was to characterize the role of IncRNA TUG1 in mouse pancreatic β cell functioning both in vitro and in vivo. Methods: qRT-PCR analyses were performed to detect the expression of lncRNA TUG1 in different tissues. RNAi, MTT, TUNEL and Annexin V-FITC assays and western blot, GSIS, ELISA and immunochemistry analyses were performed to detect the effect of lncRNA TUG1 on cell apoptosis and insulin secretion in vitro and in vivo. Results: lncRNA TUG1 was highly expressed in pancreatic tissue compared with other organ tissues, and expression was dynamically regulated by glucose in Nit-1 cells. Knockdown of lncRNA TUG1 expression resulted in an increased apoptosis ratio and decreased insulin secretion in β cells both in vitro and in vivo . Immunochemistry analyses suggested decreased relative islet area after treatment with lncRNA TUG1 siRNA. Conclusion: Downregulation of lncRNA TUG1 expression affected apoptosis and insulin secretion in pancreatic β cells in vitro and in vivo. lncRNA TUG1 may represent a factor that regulates the function of pancreatic β cells.http://www.karger.com/Article/FullText/373999ApoptosisInsulin synthesisInsulin secretionIncRNA TUG1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dan-dan Yin Er-bao Zhang Liang-hui You Ning Wang Lin-tao Wang Fei-yan Jin Ya-nan Zhu Li-hua Cao Qing-xin Yuan Wei De Wei Tang |
spellingShingle |
Dan-dan Yin Er-bao Zhang Liang-hui You Ning Wang Lin-tao Wang Fei-yan Jin Ya-nan Zhu Li-hua Cao Qing-xin Yuan Wei De Wei Tang Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β Cells Cellular Physiology and Biochemistry Apoptosis Insulin synthesis Insulin secretion IncRNA TUG1 |
author_facet |
Dan-dan Yin Er-bao Zhang Liang-hui You Ning Wang Lin-tao Wang Fei-yan Jin Ya-nan Zhu Li-hua Cao Qing-xin Yuan Wei De Wei Tang |
author_sort |
Dan-dan Yin |
title |
Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β Cells |
title_short |
Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β Cells |
title_full |
Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β Cells |
title_fullStr |
Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β Cells |
title_full_unstemmed |
Downregulation of lncRNA TUG1 Affects Apoptosis and Insulin Secretion in Mouse Pancreatic β Cells |
title_sort |
downregulation of lncrna tug1 affects apoptosis and insulin secretion in mouse pancreatic β cells |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2015-03-01 |
description |
Background: Increasing evidence indicates that long noncoding RNAs (IncRNAs) perform specific biological functions in diverse processes. Recent studies have reported that IncRNAs may be involved in β cell function. The aim of this study was to characterize the role of IncRNA TUG1 in mouse pancreatic β cell functioning both in vitro and in vivo. Methods: qRT-PCR analyses were performed to detect the expression of lncRNA TUG1 in different tissues. RNAi, MTT, TUNEL and Annexin V-FITC assays and western blot, GSIS, ELISA and immunochemistry analyses were performed to detect the effect of lncRNA TUG1 on cell apoptosis and insulin secretion in vitro and in vivo. Results: lncRNA TUG1 was highly expressed in pancreatic tissue compared with other organ tissues, and expression was dynamically regulated by glucose in Nit-1 cells. Knockdown of lncRNA TUG1 expression resulted in an increased apoptosis ratio and decreased insulin secretion in β cells both in vitro and in vivo . Immunochemistry analyses suggested decreased relative islet area after treatment with lncRNA TUG1 siRNA. Conclusion: Downregulation of lncRNA TUG1 expression affected apoptosis and insulin secretion in pancreatic β cells in vitro and in vivo. lncRNA TUG1 may represent a factor that regulates the function of pancreatic β cells. |
topic |
Apoptosis Insulin synthesis Insulin secretion IncRNA TUG1 |
url |
http://www.karger.com/Article/FullText/373999 |
work_keys_str_mv |
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