Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study
Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alp...
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Universidade Estadual de Campinas
2015-10-01
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doaj-2952e9c6b0d24d3a8548401178a2a9622021-07-15T14:03:33ZengUniversidade Estadual de CampinasBrazilian Journal of Oral Sciences1677-32252015-10-0112410.20396/bjos.v12i4.8640941Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical studyPatricio Vildósola Grez0Patricia Palma Fluxa1Paula Zuñiga Saavedra2Eduardo Fernandez Godoy3Osmir Batista de Oliveira Junior4Gustavo Moncada Cortés5University of ChileUniversity of ChileUniversity of ChileUniversity of ChileUniversidade Estadual PaulistaUniversity of ChileAim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge’s criteria (examiner´ Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 ºC for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group.https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640941Streptococcus mutansDental cariesDental restoration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Patricio Vildósola Grez Patricia Palma Fluxa Paula Zuñiga Saavedra Eduardo Fernandez Godoy Osmir Batista de Oliveira Junior Gustavo Moncada Cortés |
spellingShingle |
Patricio Vildósola Grez Patricia Palma Fluxa Paula Zuñiga Saavedra Eduardo Fernandez Godoy Osmir Batista de Oliveira Junior Gustavo Moncada Cortés Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study Brazilian Journal of Oral Sciences Streptococcus mutans Dental caries Dental restoration |
author_facet |
Patricio Vildósola Grez Patricia Palma Fluxa Paula Zuñiga Saavedra Eduardo Fernandez Godoy Osmir Batista de Oliveira Junior Gustavo Moncada Cortés |
author_sort |
Patricio Vildósola Grez |
title |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title_short |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title_full |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title_fullStr |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title_full_unstemmed |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title_sort |
recovering streptococcus mutans over restorations by the tray technique - a randomized clinical study |
publisher |
Universidade Estadual de Campinas |
series |
Brazilian Journal of Oral Sciences |
issn |
1677-3225 |
publishDate |
2015-10-01 |
description |
Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge’s criteria (examiner´ Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 ºC for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group. |
topic |
Streptococcus mutans Dental caries Dental restoration |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640941 |
work_keys_str_mv |
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