Improved efficiency and robustness in qPCR and multiplex end-point PCR by twisted intercalating nucleic acid modified primers.

Improved efficiency and robustness in qPCR and multiplex end-point PCR by twisted intercalating nucleic acid modified primers.

We introduce quantitative polymerase chain reaction (qPCR) primers and multiplex end-point PCR primers modified by the addition of a single ortho-Twisted Intercalating Nucleic Acid (o-TINA) molecule at the 5'-end. In qPCR, the 5'-o-TINA modified primers allow for a qPCR efficiency of 100%...

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Bibliographic Details
Main Authors: Uffe Vest Schneider, Nikolaj Dam Mikkelsen, Anja Lindqvist, Limei Meng Okkels, Nina Jøhnk, Gorm Lisby
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3368873?pdf=render

Internet

http://europepmc.org/articles/PMC3368873?pdf=render

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