Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT

Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT

Aim. To establish correlation between CD34+ autologous hematopoietic stem cell (HSC) count and colony-forming units (CFU) in the same peripheral blood apheresis product samples before and after cryopreservation in multiple myeloma and lymphoma patients, and to assess clinical value of these paramete...

Full description

Bibliographic Details
Main Authors: VA Balashova, VI Rugal’, SS Bessmel’tsev, SV Gritsaev, NYu Semenova, SV Voloshin, ZhV Chubukina, AV Shmidt, AD Garifullin, IM Zapreeva, AA Kuzyaeva, II Kostroma, AYu Kuvshinov, AV Chechetkin
Format: Article
Language:Russian
Published: Practical Medicine Publishing House 2018-10-01
Series:Kliničeskaâ onkogematologiâ
Subjects:
CD34+ cells
CFU
CFU-GM
correlation
lymphoma
multiple myeloma
apheresis
auto-HSCT
Online Access:http://bloodjournal.ru/wp-content/uploads/2018/09/12-1.pdf
id doaj-2a481b55a04f4a729c12e4223e6e99b1
record_format Article
spelling doaj-2a481b55a04f4a729c12e4223e6e99b12020-11-24T21:41:29ZrusPractical Medicine Publishing HouseKliničeskaâ onkogematologiâ1997-69332500-21392018-10-0111436837710.21320/2500-2139-2018-11-4-368-377Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCTVA Balashova0VI Rugal’1SS Bessmel’tsev2SV Gritsaev3NYu Semenova4SV Voloshin5ZhV Chubukina6AV Shmidt7AD Garifullin8IM Zapreeva9AA Kuzyaeva10II Kostroma11AYu Kuvshinov12AV Chechetkin13Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024Aim. To establish correlation between CD34+ autologous hematopoietic stem cell (HSC) count and colony-forming units (CFU) in the same peripheral blood apheresis product samples before and after cryopreservation in multiple myeloma and lymphoma patients, and to assess clinical value of these parameters. Materials & Methods. Cell samples of peripheral blood cytapheresis product and cell cultures were studied before and after cryopreservation in 32 multiple myeloma and 25 lymphoma patients who underwent autologous HSC transplantation. The material was analyzed using culture technique and flow cytometry. Results. The paper provides information on the relationship between CD34+ HSC count obtained by flow cytometry, and CFU in cell culture obtained by cytapheresis of the same peripheral blood samples. A direct correlation was confirmed between CD34+ count and all the CFUs before and after cryopreservation in lymphoma patients. Correlation between CD34+ count and granulocyte-macrophage CFUs was revealed in multiple myeloma and lymphoma patients before cryopreservation. Conclusion. The parameter of colony-forming capacity used for the assessment of the functional HSC was shown to be equally reliable criterion for condition evaluation of autotransplant proliferative pool than CD34+ cells. Both methods should be applied for qualitative and quantitative evaluation of an autotransplant for multiple myeloma and lymphoma patients.http://bloodjournal.ru/wp-content/uploads/2018/09/12-1.pdfCD34+ cellsCFUCFU-GMcorrelationlymphomamultiple myelomaapheresisauto-HSCT
collection DOAJ
language Russian
format Article
sources DOAJ
author VA Balashova
VI Rugal’
SS Bessmel’tsev
SV Gritsaev
NYu Semenova
SV Voloshin
ZhV Chubukina
AV Shmidt
AD Garifullin
IM Zapreeva
AA Kuzyaeva
II Kostroma
AYu Kuvshinov
AV Chechetkin
spellingShingle VA Balashova
VI Rugal’
SS Bessmel’tsev
SV Gritsaev
NYu Semenova
SV Voloshin
ZhV Chubukina
AV Shmidt
AD Garifullin
IM Zapreeva
AA Kuzyaeva
II Kostroma
AYu Kuvshinov
AV Chechetkin
Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT
Kliničeskaâ onkogematologiâ
CD34+ cells
CFU
CFU-GM
correlation
lymphoma
multiple myeloma
apheresis
auto-HSCT
author_facet VA Balashova
VI Rugal’
SS Bessmel’tsev
SV Gritsaev
NYu Semenova
SV Voloshin
ZhV Chubukina
AV Shmidt
AD Garifullin
IM Zapreeva
AA Kuzyaeva
II Kostroma
AYu Kuvshinov
AV Chechetkin
author_sort VA Balashova
title Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT
title_short Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT
title_full Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT
title_fullStr Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT
title_full_unstemmed Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT
title_sort correlation of cd34+ hematopoietic stem cells and cfu in peripheral blood apheresis products in patients with malignant lymphoproliferative diseases before and after cryopreservation prior to auto-hsct
publisher Practical Medicine Publishing House
series Kliničeskaâ onkogematologiâ
issn 1997-6933
2500-2139
publishDate 2018-10-01
description Aim. To establish correlation between CD34+ autologous hematopoietic stem cell (HSC) count and colony-forming units (CFU) in the same peripheral blood apheresis product samples before and after cryopreservation in multiple myeloma and lymphoma patients, and to assess clinical value of these parameters. Materials & Methods. Cell samples of peripheral blood cytapheresis product and cell cultures were studied before and after cryopreservation in 32 multiple myeloma and 25 lymphoma patients who underwent autologous HSC transplantation. The material was analyzed using culture technique and flow cytometry. Results. The paper provides information on the relationship between CD34+ HSC count obtained by flow cytometry, and CFU in cell culture obtained by cytapheresis of the same peripheral blood samples. A direct correlation was confirmed between CD34+ count and all the CFUs before and after cryopreservation in lymphoma patients. Correlation between CD34+ count and granulocyte-macrophage CFUs was revealed in multiple myeloma and lymphoma patients before cryopreservation. Conclusion. The parameter of colony-forming capacity used for the assessment of the functional HSC was shown to be equally reliable criterion for condition evaluation of autotransplant proliferative pool than CD34+ cells. Both methods should be applied for qualitative and quantitative evaluation of an autotransplant for multiple myeloma and lymphoma patients.
topic CD34+ cells
CFU
CFU-GM
correlation
lymphoma
multiple myeloma
apheresis
auto-HSCT
url http://bloodjournal.ru/wp-content/uploads/2018/09/12-1.pdf
work_keys_str_mv AT vabalashova correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT virugal correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT ssbessmeltsev correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT svgritsaev correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT nyusemenova correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT svvoloshin correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT zhvchubukina correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT avshmidt correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT adgarifullin correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT imzapreeva correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT aakuzyaeva correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT iikostroma correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT ayukuvshinov correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
AT avchechetkin correlationofcd34hematopoieticstemcellsandcfuinperipheralbloodapheresisproductsinpatientswithmalignantlymphoproliferativediseasesbeforeandaftercryopreservationpriortoautohsct
_version_ 1725921863106822144

Similar Items