Optimization of Organotypic Cultures of Mouse Spleen for Staining and Functional Assays

Optimization of Organotypic Cultures of Mouse Spleen for Staining and Functional Assays

By preserving cell viability and three-dimensional localization, organotypic culture stands out among the newest frontiers of cell culture. It has been successfully employed for the study of diseases among which neoplasias, where tumoral cells take advantage of the surrounding stroma to promote thei...

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Bibliographic Details
Main Authors: Francesca Finetti, Nagaja Capitani, Noemi Manganaro, Vanessa Tatangelo, Francesca Libonati, Giulia Panattoni, Ivo Calaresu, Laura Ballerini, Cosima T. Baldari, Laura Patrussi
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Immunology
Subjects:
organotypic culture
spleen
vibratome
precision-cut
white pulp
red pulp
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2020.00471/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2020.00471/full

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