Phototherapy Effect of Multifunctional Nano-cerium Oxide Quantum Dots on HepG2 Cells
Objective To investigate the phototherapy effect of multifunctional nanometer cerium oxide quantum dot (HACQDs-Ce6) on HepG2 cells. Methods The dissolved oxygen analyzer was used to detect the catalytic oxygen production capacity of HACQDs-Ce6. CCK-8 method, scratch test and Calcein AM/PI staining w...
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Magazine House of Cancer Research on Prevention and Treatment
2020-08-01
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doaj-2cb12b3b4bb94919b0d71d37769af8282020-11-25T03:55:03ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782020-08-0147857357710.3971/j.issn.1000-8578.2020.19.15348578.2020.19.1534Phototherapy Effect of Multifunctional Nano-cerium Oxide Quantum Dots on HepG2 CellsZHANG Lidong0LI Hong1CHEN Jun2HUANG Ke3MAO Yinghua4RONG Shu5WANG Chunhui6Department of Preventive Medicine, Army Medical University, Chongqing 400038, ChinaInstitute of Medical Protection, Disease Control and Prevention of Eastern Theater Command, Nanjing 210002, ChinaDepartment of Preventive Medicine, Army Medical University, Chongqing 400038, ChinaDepartment of Gynecology, Women's Hospital Affiliated to Nanjing Medical University, Nanjing 210004, ChinaInstitute of Medical Protection, Disease Control and Prevention of Eastern Theater Command, Nanjing 210002, ChinaInstitute of Medical Protection, Disease Control and Prevention of Eastern Theater Command, Nanjing 210002, ChinaInstitute of Medical Protection, Disease Control and Prevention of Eastern Theater Command, Nanjing 210002, ChinaObjective To investigate the phototherapy effect of multifunctional nanometer cerium oxide quantum dot (HACQDs-Ce6) on HepG2 cells. Methods The dissolved oxygen analyzer was used to detect the catalytic oxygen production capacity of HACQDs-Ce6. CCK-8 method, scratch test and Calcein AM/PI staining were used to analyze the effects of HACQDs-Ce6 on the proliferation, migration and apoptosis of HepG2 cells. The recombinant mouse macrophage-granulocyte colony stimulating factor (rm GM-CSF) and recombinant mouse interleukin-4 (rm IL-4) were used to induce the differentiation of mouse bone marrow precursor cells into dendritic cells (DC) in vitro to investigate the effect of HACQDs-Ce6 on DC cells. Results HACQDs-Ce6 had catalytic oxygen production capacity. The proliferation activity of cells in the laser group was significantly lower than that in the dark group when the concentration of HACQDs-Ce6 was greater than 0.125 μg/ml (P=0.018). The migration ability of HepG2 cells in HACQDs-Ce6 group was significantly lower than that in the PBS group (P < 0.01). HACQDs-Ce6 almost had no effect on HFF-1/L wnt-3A and DC cells. Calcein AM/PI staining showed that the combined action of HACQDs-Ce6 and PDT killed almost all the HepG2 cells. Conclusion The phototherapy effect of HACQDs-Ce6 on HepG2 cells is obvious, with no damage to HFF-1/L wnt-3A/DC cells.http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.1534.htmnanomaterialstumorphotodynamic therapydendritic cell |
collection |
DOAJ |
language |
zho |
format |
Article |
sources |
DOAJ |
author |
ZHANG Lidong LI Hong CHEN Jun HUANG Ke MAO Yinghua RONG Shu WANG Chunhui |
spellingShingle |
ZHANG Lidong LI Hong CHEN Jun HUANG Ke MAO Yinghua RONG Shu WANG Chunhui Phototherapy Effect of Multifunctional Nano-cerium Oxide Quantum Dots on HepG2 Cells Zhongliu Fangzhi Yanjiu nanomaterials tumor photodynamic therapy dendritic cell |
author_facet |
ZHANG Lidong LI Hong CHEN Jun HUANG Ke MAO Yinghua RONG Shu WANG Chunhui |
author_sort |
ZHANG Lidong |
title |
Phototherapy Effect of Multifunctional Nano-cerium Oxide Quantum Dots on HepG2 Cells |
title_short |
Phototherapy Effect of Multifunctional Nano-cerium Oxide Quantum Dots on HepG2 Cells |
title_full |
Phototherapy Effect of Multifunctional Nano-cerium Oxide Quantum Dots on HepG2 Cells |
title_fullStr |
Phototherapy Effect of Multifunctional Nano-cerium Oxide Quantum Dots on HepG2 Cells |
title_full_unstemmed |
Phototherapy Effect of Multifunctional Nano-cerium Oxide Quantum Dots on HepG2 Cells |
title_sort |
phototherapy effect of multifunctional nano-cerium oxide quantum dots on hepg2 cells |
publisher |
Magazine House of Cancer Research on Prevention and Treatment |
series |
Zhongliu Fangzhi Yanjiu |
issn |
1000-8578 1000-8578 |
publishDate |
2020-08-01 |
description |
Objective To investigate the phototherapy effect of multifunctional nanometer cerium oxide quantum dot (HACQDs-Ce6) on HepG2 cells. Methods The dissolved oxygen analyzer was used to detect the catalytic oxygen production capacity of HACQDs-Ce6. CCK-8 method, scratch test and Calcein AM/PI staining were used to analyze the effects of HACQDs-Ce6 on the proliferation, migration and apoptosis of HepG2 cells. The recombinant mouse macrophage-granulocyte colony stimulating factor (rm GM-CSF) and recombinant mouse interleukin-4 (rm IL-4) were used to induce the differentiation of mouse bone marrow precursor cells into dendritic cells (DC) in vitro to investigate the effect of HACQDs-Ce6 on DC cells. Results HACQDs-Ce6 had catalytic oxygen production capacity. The proliferation activity of cells in the laser group was significantly lower than that in the dark group when the concentration of HACQDs-Ce6 was greater than 0.125 μg/ml (P=0.018). The migration ability of HepG2 cells in HACQDs-Ce6 group was significantly lower than that in the PBS group (P < 0.01). HACQDs-Ce6 almost had no effect on HFF-1/L wnt-3A and DC cells. Calcein AM/PI staining showed that the combined action of HACQDs-Ce6 and PDT killed almost all the HepG2 cells. Conclusion The phototherapy effect of HACQDs-Ce6 on HepG2 cells is obvious, with no damage to HFF-1/L wnt-3A/DC cells. |
topic |
nanomaterials tumor photodynamic therapy dendritic cell |
url |
http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.1534.htm |
work_keys_str_mv |
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1724470955297734656 |