Retention behaviour of some estradiol derivatives on alumina in normal phase chromatrography
The retention constants of variously substituted estradiol derivatives were measured as a function of the composition of various binary mobile phases in order to study the relationship between chemical structure and retention behavior in TLC on alumina. The slopes and intercepts of the linear relati...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2003-01-01
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| Series: | Journal of the Serbian Chemical Society |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2003/0352-51390303163A.pdf |
| Summary: | The retention constants of variously substituted estradiol derivatives were measured as a function of the composition of various binary mobile phases in order to study the relationship between chemical structure and retention behavior in TLC on alumina. The slopes and intercepts of the linear relationships between the retention constant RM and the logarithm of the volume fraction of the polar mobile phase component were calculated and are discussed in relation to the characteristics of the solute and mobile phase. The RF values and relative retention RM of the compounds depend largely on the retention behavior of their substituents. The results obtained in this investigation are compared with the results of the same derivatives obtained in previous investigations on silica gel. |
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| ISSN: | 0352-5139 1820-7421 |