Frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity

Background: One of the causative factors in development of dental caries is microorganisms. Two species of Mutans streptococci including Streptococcus mutans and Streptococcus sobrinus are associated with dental caries in human beings. The aim of this study was to investigate the frequency of S. mut...

Full description

Bibliographic Details
Main Authors: Maryam Ghasempour, Ramazan Rajabnia, Azin Irannejad, Mahtab Hamzeh, Elahe Ferdosi, Meghdad Bagheri
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2013-01-01
Series:Dental Research Journal
Subjects:
Online Access:http://www.drjjournal.net/article.asp?issn=1735-3327;year=2013;volume=10;issue=4;spage=440;epage=445;aulast=Ghasempour
id doaj-2d171fc8b0f44fa8a005d4919a8132fb
record_format Article
spelling doaj-2d171fc8b0f44fa8a005d4919a8132fb2020-11-24T21:06:55ZengWolters Kluwer Medknow PublicationsDental Research Journal1735-33272008-02552013-01-0110444044510.4103/1735-3327.118478Frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activityMaryam GhasempourRamazan RajabniaAzin IrannejadMahtab HamzehElahe FerdosiMeghdad BagheriBackground: One of the causative factors in development of dental caries is microorganisms. Two species of Mutans streptococci including Streptococcus mutans and Streptococcus sobrinus are associated with dental caries in human beings. The aim of this study was to investigate the frequency of S. mutans and S. sobrinus in saliva of children with different caries activity and ability to form biofilm and acid susceptibility of these microorganisms. Materials and Methods: This analytical case-control study was performed on 83 preschool children, 4-6 years old. Children were divided into two groups including 41 caries-active and 42 caries-free children. Non-stimulated saliva samples were collected and culture and polymerase chain reaction techniques were used. Statistical analysis was performed using t-test, Chi-square, ANOVA, and Kappa tests. Results: S. mutans and S. sobrinus were found in 65% and 21.6% of the samples respectively. S. mutans was isolated from 75.6% of caries-active and 54.8% of caries-free children. Figures for S. sobrinus were 29.2% and 14.3% respectively. Acid susceptibility of microorganisms isolated from saliva was 87.43 in caries-active children and 94.30 for caries-free children. Biofilm formation of microorganisms in caries-active and caries-free children was 0.77 and 0.73, respectively. Conclusion: Frequency of S. mutans in caries-active children was significantly higher than caries-free children, but the difference in frequency of S. sobrinus was not significant. Acid susceptibility of microorganisms in caries-active children was significantly lower, but the ability to form biofilm was not significantly different in two groups.http://www.drjjournal.net/article.asp?issn=1735-3327;year=2013;volume=10;issue=4;spage=440;epage=445;aulast=GhasempourAcid susceptibilitybiofilmStreptococcus mutansStreptococcus sobrinus
collection DOAJ
language English
format Article
sources DOAJ
author Maryam Ghasempour
Ramazan Rajabnia
Azin Irannejad
Mahtab Hamzeh
Elahe Ferdosi
Meghdad Bagheri
spellingShingle Maryam Ghasempour
Ramazan Rajabnia
Azin Irannejad
Mahtab Hamzeh
Elahe Ferdosi
Meghdad Bagheri
Frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity
Dental Research Journal
Acid susceptibility
biofilm
Streptococcus mutans
Streptococcus sobrinus
author_facet Maryam Ghasempour
Ramazan Rajabnia
Azin Irannejad
Mahtab Hamzeh
Elahe Ferdosi
Meghdad Bagheri
author_sort Maryam Ghasempour
title Frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity
title_short Frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity
title_full Frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity
title_fullStr Frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity
title_full_unstemmed Frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity
title_sort frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity
publisher Wolters Kluwer Medknow Publications
series Dental Research Journal
issn 1735-3327
2008-0255
publishDate 2013-01-01
description Background: One of the causative factors in development of dental caries is microorganisms. Two species of Mutans streptococci including Streptococcus mutans and Streptococcus sobrinus are associated with dental caries in human beings. The aim of this study was to investigate the frequency of S. mutans and S. sobrinus in saliva of children with different caries activity and ability to form biofilm and acid susceptibility of these microorganisms. Materials and Methods: This analytical case-control study was performed on 83 preschool children, 4-6 years old. Children were divided into two groups including 41 caries-active and 42 caries-free children. Non-stimulated saliva samples were collected and culture and polymerase chain reaction techniques were used. Statistical analysis was performed using t-test, Chi-square, ANOVA, and Kappa tests. Results: S. mutans and S. sobrinus were found in 65% and 21.6% of the samples respectively. S. mutans was isolated from 75.6% of caries-active and 54.8% of caries-free children. Figures for S. sobrinus were 29.2% and 14.3% respectively. Acid susceptibility of microorganisms isolated from saliva was 87.43 in caries-active children and 94.30 for caries-free children. Biofilm formation of microorganisms in caries-active and caries-free children was 0.77 and 0.73, respectively. Conclusion: Frequency of S. mutans in caries-active children was significantly higher than caries-free children, but the difference in frequency of S. sobrinus was not significant. Acid susceptibility of microorganisms in caries-active children was significantly lower, but the ability to form biofilm was not significantly different in two groups.
topic Acid susceptibility
biofilm
Streptococcus mutans
Streptococcus sobrinus
url http://www.drjjournal.net/article.asp?issn=1735-3327;year=2013;volume=10;issue=4;spage=440;epage=445;aulast=Ghasempour
work_keys_str_mv AT maryamghasempour frequencybiofilmformationandacidsusceptibilityofstreptococcusmutansandstreptococcussobrinusinsalivaofpreschoolchildrenwithdifferentlevelsofcariesactivity
AT ramazanrajabnia frequencybiofilmformationandacidsusceptibilityofstreptococcusmutansandstreptococcussobrinusinsalivaofpreschoolchildrenwithdifferentlevelsofcariesactivity
AT azinirannejad frequencybiofilmformationandacidsusceptibilityofstreptococcusmutansandstreptococcussobrinusinsalivaofpreschoolchildrenwithdifferentlevelsofcariesactivity
AT mahtabhamzeh frequencybiofilmformationandacidsusceptibilityofstreptococcusmutansandstreptococcussobrinusinsalivaofpreschoolchildrenwithdifferentlevelsofcariesactivity
AT elaheferdosi frequencybiofilmformationandacidsusceptibilityofstreptococcusmutansandstreptococcussobrinusinsalivaofpreschoolchildrenwithdifferentlevelsofcariesactivity
AT meghdadbagheri frequencybiofilmformationandacidsusceptibilityofstreptococcusmutansandstreptococcussobrinusinsalivaofpreschoolchildrenwithdifferentlevelsofcariesactivity
_version_ 1716764266058481664