Effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats

Background: An anti-cariogenic diet containing probiotics can be effective in caries prevention. This animal study compared the effects of milk and yogurt on Streptococcus sobrinus counts and caries score. Materials and Methods: A total of 36 male rats were infected with S. sobrinus (27,607) and di...

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Main Authors: Maryam Ghasempour, Ramazam Rajabnia, Manouchehr Ashrafpour, Atiyyeh Ehsani, Ali Akbar Moghadamnia, Samane Gharekhani, Elaheh Ferdosi Shahandashti, Meghdad Bagheri
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2015-01-01
Series:Dental Research Journal
Subjects:
Online Access:http://www.drjjournal.net/article.asp?issn=1735-3327;year=2015;volume=12;issue=6;spage=569;epage=573;aulast=Ghasempour
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spelling doaj-2e30953e00f748f28916693097f301602020-11-24T22:48:06ZengWolters Kluwer Medknow PublicationsDental Research Journal1735-33272008-02552015-01-0112656957310.4103/1735-3327.170577Effect of milk and yogurt on streptococcus sobrinus counts and caries score in ratsMaryam GhasempourRamazam RajabniaManouchehr AshrafpourAtiyyeh EhsaniAli Akbar MoghadamniaSamane GharekhaniElaheh Ferdosi ShahandashtiMeghdad BagheriBackground: An anti-cariogenic diet containing probiotics can be effective in caries prevention. This animal study compared the effects of milk and yogurt on Streptococcus sobrinus counts and caries score. Materials and Methods: A total of 36 male rats were infected with S. sobrinus (27,607) and divided into three groups. Group A and B received 200 mL of milk and 100 g of yogurt per day, respectively, and a control group received 2.5 mL of NCP number 2 diet twice daily for 21 days. After killing the animals, their lower left jaws were removed and sonicated to quantify the colonies of S. sobrinus. Dental caries was scored using Keyes technique. Data were analyzed using ANOVA and Kruskal-Wallis, Mann-Whitney and Wilcoxon-Signed Rank tests. Statistical significance was set at P < 0.05. Results: The mean (±standard error of the mean) of S. sobrinus colonies in the milk, yogurt and control groups were determined at 119666.67 (±20733), 46416.666 (±12846) and 163,250 (±33493), respectively. Microbial counts decreased in the yogurt group compared with the milk and control groups (P = 0.004 and P = 0.000; respectively). There were significant differences between caries scores of smooth surfaces in the milk and yogurt groups compared with the control group (P = 0.000 and P = 0.000, respectively). Both milk and yogurt significantly reduced caries score of fissured surfaces compared with controls (P = 0.004 and P = 0.000, respectively). Conclusion: Considering the limitations of this study, yogurt administration reduces S. sobrinus counts. In addition, yogurt and milk regimens reduce the caries scores of smooth and fissured surfaces.http://www.drjjournal.net/article.asp?issn=1735-3327;year=2015;volume=12;issue=6;spage=569;epage=573;aulast=GhasempourDental cariesmilkStreptococcus sobrinusyogurt
collection DOAJ
language English
format Article
sources DOAJ
author Maryam Ghasempour
Ramazam Rajabnia
Manouchehr Ashrafpour
Atiyyeh Ehsani
Ali Akbar Moghadamnia
Samane Gharekhani
Elaheh Ferdosi Shahandashti
Meghdad Bagheri
spellingShingle Maryam Ghasempour
Ramazam Rajabnia
Manouchehr Ashrafpour
Atiyyeh Ehsani
Ali Akbar Moghadamnia
Samane Gharekhani
Elaheh Ferdosi Shahandashti
Meghdad Bagheri
Effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats
Dental Research Journal
Dental caries
milk
Streptococcus sobrinus
yogurt
author_facet Maryam Ghasempour
Ramazam Rajabnia
Manouchehr Ashrafpour
Atiyyeh Ehsani
Ali Akbar Moghadamnia
Samane Gharekhani
Elaheh Ferdosi Shahandashti
Meghdad Bagheri
author_sort Maryam Ghasempour
title Effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats
title_short Effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats
title_full Effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats
title_fullStr Effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats
title_full_unstemmed Effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats
title_sort effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats
publisher Wolters Kluwer Medknow Publications
series Dental Research Journal
issn 1735-3327
2008-0255
publishDate 2015-01-01
description Background: An anti-cariogenic diet containing probiotics can be effective in caries prevention. This animal study compared the effects of milk and yogurt on Streptococcus sobrinus counts and caries score. Materials and Methods: A total of 36 male rats were infected with S. sobrinus (27,607) and divided into three groups. Group A and B received 200 mL of milk and 100 g of yogurt per day, respectively, and a control group received 2.5 mL of NCP number 2 diet twice daily for 21 days. After killing the animals, their lower left jaws were removed and sonicated to quantify the colonies of S. sobrinus. Dental caries was scored using Keyes technique. Data were analyzed using ANOVA and Kruskal-Wallis, Mann-Whitney and Wilcoxon-Signed Rank tests. Statistical significance was set at P < 0.05. Results: The mean (±standard error of the mean) of S. sobrinus colonies in the milk, yogurt and control groups were determined at 119666.67 (±20733), 46416.666 (±12846) and 163,250 (±33493), respectively. Microbial counts decreased in the yogurt group compared with the milk and control groups (P = 0.004 and P = 0.000; respectively). There were significant differences between caries scores of smooth surfaces in the milk and yogurt groups compared with the control group (P = 0.000 and P = 0.000, respectively). Both milk and yogurt significantly reduced caries score of fissured surfaces compared with controls (P = 0.004 and P = 0.000, respectively). Conclusion: Considering the limitations of this study, yogurt administration reduces S. sobrinus counts. In addition, yogurt and milk regimens reduce the caries scores of smooth and fissured surfaces.
topic Dental caries
milk
Streptococcus sobrinus
yogurt
url http://www.drjjournal.net/article.asp?issn=1735-3327;year=2015;volume=12;issue=6;spage=569;epage=573;aulast=Ghasempour
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