lncRNA-Six1 Is a Target of miR-1611 That Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis

lncRNA-Six1 Is a Target of miR-1611 That Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis

Emerging studies indicate important roles for non-coding RNAs (ncRNAs) as essential regulators in myogenesis, but relatively less is known about their function. In our previous study, we found that lncRNA-Six1 can regulate <i>Six1</i> in <i>cis</i> to participate in myogenesi...

Full description

Bibliographic Details
Main Authors: Manting Ma, Bolin Cai, Liang Jiang, Bahareldin Ali Abdalla, Zhenhui Li, Qinghua Nie, Xiquan Zhang
Format: Article
Language:English
Published: MDPI AG 2018-12-01
Series:Cells
Subjects:
miR-1611
lncRNA-Six1
<i>Six1</i>
myoblast proliferation
myoblast differentiation
transformation of muscle fiber types
Online Access:https://www.mdpi.com/2073-4409/7/12/243
id doaj-2e9e3e6c7c484c1d9996c84160e7fb3d
record_format Article
spelling doaj-2e9e3e6c7c484c1d9996c84160e7fb3d2020-11-24T20:42:46ZengMDPI AGCells2073-44092018-12-0171224310.3390/cells7120243cells7120243lncRNA-Six1 Is a Target of miR-1611 That Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken MyogenesisManting Ma0Bolin Cai1Liang Jiang2Bahareldin Ali Abdalla3Zhenhui Li4Qinghua Nie5Xiquan Zhang6Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, ChinaEmerging studies indicate important roles for non-coding RNAs (ncRNAs) as essential regulators in myogenesis, but relatively less is known about their function. In our previous study, we found that lncRNA-Six1 can regulate <i>Six1</i> in <i>cis</i> to participate in myogenesis. Here, we studied a microRNA (miRNA) that is specifically expressed in chickens (miR-1611). Interestingly, miR-1611 was found to contain potential binding sites for both lncRNA-Six1 and <i>Six1</i>, and it can interact with lncRNA-Six1 to regulate <i>Six1</i> expression. Overexpression of miR-1611 represses the proliferation and differentiation of myoblasts. Moreover, miR-1611 is highly expressed in slow-twitch fibers, and it drives the transformation of fast-twitch muscle fibers to slow-twitch muscle fibers. Together, these data demonstrate that miR-1611 can mediate the regulation of Six1 by lncRNA-Six1, thereby affecting proliferation and differentiation of myoblasts and transformation of muscle fiber types.https://www.mdpi.com/2073-4409/7/12/243miR-1611lncRNA-Six1<i>Six1</i>myoblast proliferationmyoblast differentiationtransformation of muscle fiber types
collection DOAJ
language English
format Article
sources DOAJ
author Manting Ma
Bolin Cai
Liang Jiang
Bahareldin Ali Abdalla
Zhenhui Li
Qinghua Nie
Xiquan Zhang
spellingShingle Manting Ma
Bolin Cai
Liang Jiang
Bahareldin Ali Abdalla
Zhenhui Li
Qinghua Nie
Xiquan Zhang
lncRNA-Six1 Is a Target of miR-1611 That Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis
Cells
miR-1611
lncRNA-Six1
<i>Six1</i>
myoblast proliferation
myoblast differentiation
transformation of muscle fiber types
author_facet Manting Ma
Bolin Cai
Liang Jiang
Bahareldin Ali Abdalla
Zhenhui Li
Qinghua Nie
Xiquan Zhang
author_sort Manting Ma
title lncRNA-Six1 Is a Target of miR-1611 That Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis
title_short lncRNA-Six1 Is a Target of miR-1611 That Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis
title_full lncRNA-Six1 Is a Target of miR-1611 That Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis
title_fullStr lncRNA-Six1 Is a Target of miR-1611 That Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis
title_full_unstemmed lncRNA-Six1 Is a Target of miR-1611 That Functions as a ceRNA to Regulate Six1 Protein Expression and Fiber Type Switching in Chicken Myogenesis
title_sort lncrna-six1 is a target of mir-1611 that functions as a cerna to regulate six1 protein expression and fiber type switching in chicken myogenesis
publisher MDPI AG
series Cells
issn 2073-4409
publishDate 2018-12-01
description Emerging studies indicate important roles for non-coding RNAs (ncRNAs) as essential regulators in myogenesis, but relatively less is known about their function. In our previous study, we found that lncRNA-Six1 can regulate <i>Six1</i> in <i>cis</i> to participate in myogenesis. Here, we studied a microRNA (miRNA) that is specifically expressed in chickens (miR-1611). Interestingly, miR-1611 was found to contain potential binding sites for both lncRNA-Six1 and <i>Six1</i>, and it can interact with lncRNA-Six1 to regulate <i>Six1</i> expression. Overexpression of miR-1611 represses the proliferation and differentiation of myoblasts. Moreover, miR-1611 is highly expressed in slow-twitch fibers, and it drives the transformation of fast-twitch muscle fibers to slow-twitch muscle fibers. Together, these data demonstrate that miR-1611 can mediate the regulation of Six1 by lncRNA-Six1, thereby affecting proliferation and differentiation of myoblasts and transformation of muscle fiber types.
topic miR-1611
lncRNA-Six1
<i>Six1</i>
myoblast proliferation
myoblast differentiation
transformation of muscle fiber types
url https://www.mdpi.com/2073-4409/7/12/243
work_keys_str_mv AT mantingma lncrnasix1isatargetofmir1611thatfunctionsasacernatoregulatesix1proteinexpressionandfibertypeswitchinginchickenmyogenesis
AT bolincai lncrnasix1isatargetofmir1611thatfunctionsasacernatoregulatesix1proteinexpressionandfibertypeswitchinginchickenmyogenesis
AT liangjiang lncrnasix1isatargetofmir1611thatfunctionsasacernatoregulatesix1proteinexpressionandfibertypeswitchinginchickenmyogenesis
AT bahareldinaliabdalla lncrnasix1isatargetofmir1611thatfunctionsasacernatoregulatesix1proteinexpressionandfibertypeswitchinginchickenmyogenesis
AT zhenhuili lncrnasix1isatargetofmir1611thatfunctionsasacernatoregulatesix1proteinexpressionandfibertypeswitchinginchickenmyogenesis
AT qinghuanie lncrnasix1isatargetofmir1611thatfunctionsasacernatoregulatesix1proteinexpressionandfibertypeswitchinginchickenmyogenesis
AT xiquanzhang lncrnasix1isatargetofmir1611thatfunctionsasacernatoregulatesix1proteinexpressionandfibertypeswitchinginchickenmyogenesis
_version_ 1716821794051063808

Similar Items