Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus

Classical swine fever (CSF) is a highly contagious swine infectious disease and causes significant economic losses for the pig industry worldwide. The objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of CSF. Birefly, serum metabolomics of CSF...

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Main Authors: Jishu Shi, Changchun Tu, Wenjie Gong, Junjie Jia, Bikai Zhang, Shijiang Mi, Li Zhang, Xiaoming Xie, Huancheng Guo
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-04-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.00731/full
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spelling doaj-2ff3e574ed5b4f52acebe67ec536b2fe2020-11-25T01:07:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-04-01810.3389/fmicb.2017.00731258719Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever VirusJishu Shi0Changchun Tu1Changchun Tu2Wenjie Gong3Wenjie Gong4Junjie Jia5Bikai Zhang6Shijiang Mi7Li Zhang8Xiaoming Xie9Huancheng Guo10Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State UniversityManhattan, KS, USADepartment of Virology, Institute of Military Veterinary, Academy of Military Medical SciencesChangchun, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou, ChinaDepartment of Virology, Institute of Military Veterinary, Academy of Military Medical SciencesChangchun, ChinaDepartment of Anatomy and Physiology, College of Veterinary Medicine, Kansas State UniversityManhattan, KS, USADepartment of Virology, Institute of Military Veterinary, Academy of Military Medical SciencesChangchun, ChinaDepartment of Virology, Institute of Military Veterinary, Academy of Military Medical SciencesChangchun, ChinaDepartment of Virology, Institute of Military Veterinary, Academy of Military Medical SciencesChangchun, ChinaDepartment of Virology, Institute of Military Veterinary, Academy of Military Medical SciencesChangchun, ChinaDepartment of Virology, Institute of Military Veterinary, Academy of Military Medical SciencesChangchun, ChinaDepartment of Virology, Institute of Military Veterinary, Academy of Military Medical SciencesChangchun, ChinaClassical swine fever (CSF) is a highly contagious swine infectious disease and causes significant economic losses for the pig industry worldwide. The objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of CSF. Birefly, serum metabolomics of CSFV Shimen strain-infected piglets were analyzed by ultraperformance liquid chromatography/electrospray ionization time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) in combination with multivariate statistical analysis. In CSFV-infected piglets at days 3 and 7 post-infection changes were found in metabolites associated with several key metabolic pathways, including tryptophan catabolism and the kynurenine pathway, phenylalanine metabolism, fatty acid and lipid metabolism, the tricarboxylic acid and urea cycles, branched-chain amino acid metabolism, and nucleotide metabolism. Several pathways involved in energy metabolism including fatty acid biosynthesis and β-oxidation, branched-chain amino acid metabolism, and the tricarboxylic acid cycle were significantly inhibited. Changes were also observed in several metabolites exclusively associated with gut microbiota. The metabolomic profiles indicate that CSFV-host gut microbiome interactions play a role in the development of CSF.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00731/fullmetabolomic profilingUPLC-MSCSFVmetabolite
collection DOAJ
language English
format Article
sources DOAJ
author Jishu Shi
Changchun Tu
Changchun Tu
Wenjie Gong
Wenjie Gong
Junjie Jia
Bikai Zhang
Shijiang Mi
Li Zhang
Xiaoming Xie
Huancheng Guo
spellingShingle Jishu Shi
Changchun Tu
Changchun Tu
Wenjie Gong
Wenjie Gong
Junjie Jia
Bikai Zhang
Shijiang Mi
Li Zhang
Xiaoming Xie
Huancheng Guo
Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus
Frontiers in Microbiology
metabolomic profiling
UPLC-MS
CSFV
metabolite
author_facet Jishu Shi
Changchun Tu
Changchun Tu
Wenjie Gong
Wenjie Gong
Junjie Jia
Bikai Zhang
Shijiang Mi
Li Zhang
Xiaoming Xie
Huancheng Guo
author_sort Jishu Shi
title Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus
title_short Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus
title_full Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus
title_fullStr Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus
title_full_unstemmed Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus
title_sort serum metabolomic profiling of piglets infected with virulent classical swine fever virus
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2017-04-01
description Classical swine fever (CSF) is a highly contagious swine infectious disease and causes significant economic losses for the pig industry worldwide. The objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of CSF. Birefly, serum metabolomics of CSFV Shimen strain-infected piglets were analyzed by ultraperformance liquid chromatography/electrospray ionization time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) in combination with multivariate statistical analysis. In CSFV-infected piglets at days 3 and 7 post-infection changes were found in metabolites associated with several key metabolic pathways, including tryptophan catabolism and the kynurenine pathway, phenylalanine metabolism, fatty acid and lipid metabolism, the tricarboxylic acid and urea cycles, branched-chain amino acid metabolism, and nucleotide metabolism. Several pathways involved in energy metabolism including fatty acid biosynthesis and β-oxidation, branched-chain amino acid metabolism, and the tricarboxylic acid cycle were significantly inhibited. Changes were also observed in several metabolites exclusively associated with gut microbiota. The metabolomic profiles indicate that CSFV-host gut microbiome interactions play a role in the development of CSF.
topic metabolomic profiling
UPLC-MS
CSFV
metabolite
url http://journal.frontiersin.org/article/10.3389/fmicb.2017.00731/full
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