NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles

Abstract Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione coro...

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Main Authors:	Antonio Buonerba, Rosita Lapenta, Anna Donniacuo, Magda Licasale, Elena Vezzoli, Stefano Milione, Carmine Capacchione, Mario Felice Tecce, Andrea Falqui, Roberto Piacentini, Claudio Grassi, Alfonso Grassi
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-07-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-020-68397-1

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spelling	doaj-300c905aafb8441c8456aaf3c71700cf2021-07-11T11:22:17ZengNature Publishing GroupScientific Reports2045-23222020-07-0110111410.1038/s41598-020-68397-1NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticlesAntonio Buonerba0Rosita Lapenta1Anna Donniacuo2Magda Licasale3Elena Vezzoli4Stefano Milione5Carmine Capacchione6Mario Felice Tecce7Andrea Falqui8Roberto Piacentini9Claudio Grassi10Alfonso Grassi11Sanitary Environmental Engineering Division (SEED), and Consorzio Inter-universitario Previsione e Prevenzione dei Grandi Rischi (Cu.G.Ri.), Department of Civil Engineering, University of SalernoDepartment of Chemistry and Biology “Adolfo Zambelli”, University of SalernoDepartment of Chemistry and Biology “Adolfo Zambelli”, University of SalernoDepartment of Chemistry and Biology “Adolfo Zambelli”, University of SalernoBiological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST)Department of Chemistry and Biology “Adolfo Zambelli”, University of SalernoDepartment of Chemistry and Biology “Adolfo Zambelli”, University of SalernoDepartment of Pharmacy, University of SalernoBiological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST)Department of Neuroscience, Università Cattolica del Sacro CuoreDepartment of Neuroscience, Università Cattolica del Sacro CuoreDepartment of Chemistry and Biology “Adolfo Zambelli”, University of SalernoAbstract Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione corona functionalized with the dansyl chromophore (a-DG-AuNPs) were synthesized and found efficient nanodevice for applications in photothermal therapy (PTT). Actually the nanoparticle aggregation enhances the quenching of radiative excitation and the consequent conversion into heat. The a-DG-AuNPs are readily internalized in Hep G2 where the chromophore acts as both antenna and transducer of the NIR radiation under two-photons excitation, determining efficient cell ablation via photothermal effect.https://doi.org/10.1038/s41598-020-68397-1
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Antonio Buonerba Rosita Lapenta Anna Donniacuo Magda Licasale Elena Vezzoli Stefano Milione Carmine Capacchione Mario Felice Tecce Andrea Falqui Roberto Piacentini Claudio Grassi Alfonso Grassi
spellingShingle	Antonio Buonerba Rosita Lapenta Anna Donniacuo Magda Licasale Elena Vezzoli Stefano Milione Carmine Capacchione Mario Felice Tecce Andrea Falqui Roberto Piacentini Claudio Grassi Alfonso Grassi NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles Scientific Reports
author_facet	Antonio Buonerba Rosita Lapenta Anna Donniacuo Magda Licasale Elena Vezzoli Stefano Milione Carmine Capacchione Mario Felice Tecce Andrea Falqui Roberto Piacentini Claudio Grassi Alfonso Grassi
author_sort	Antonio Buonerba
title	NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles
title_short	NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles
title_full	NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles
title_fullStr	NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles
title_full_unstemmed	NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles
title_sort	nir multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2020-07-01
description	Abstract Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione corona functionalized with the dansyl chromophore (a-DG-AuNPs) were synthesized and found efficient nanodevice for applications in photothermal therapy (PTT). Actually the nanoparticle aggregation enhances the quenching of radiative excitation and the consequent conversion into heat. The a-DG-AuNPs are readily internalized in Hep G2 where the chromophore acts as both antenna and transducer of the NIR radiation under two-photons excitation, determining efficient cell ablation via photothermal effect.
url	https://doi.org/10.1038/s41598-020-68397-1
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NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles

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