Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species
Adult Lymnaea acuminata (average length 20-22 mm) were collected locally from lakes and low-lying submerged fields from Gorakhpur. The chemoattraction studies were made in round glass aquaria measuring 30 cm in diameter and filled to a depth of 10 mm with 500 ml dechlorinated tap water. Each aquariu...
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2004-01-01
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doaj-32bb8bf82b27434e9ee3053985153c272020-11-24T23:55:51ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73102004-01-01374587590Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola speciesTiwari F.Singh D.K.Adult Lymnaea acuminata (average length 20-22 mm) were collected locally from lakes and low-lying submerged fields from Gorakhpur. The chemoattraction studies were made in round glass aquaria measuring 30 cm in diameter and filled to a depth of 10 mm with 500 ml dechlorinated tap water. Each aquarium was divided into four concentric zones. At the starting time of the assay 10 snails were placed on the circumference of outermost zone 0. Snail attractant pellets (SAP) were added simultaneously in the center of central zone 3. SAP of different amino acids were prepared at concentrations of 10, 20, 50, 80 and 100 mM/2% agar solution and, subsequently, spread to a uniform thickness of 5 mm. After cooling, SAP were cut in small pieces of 5 mm in diameter. Lymnaea acuminata's attraction to amino acids was studied using different amino acid concentrations in SAP. Pellets containing amino acids with non-polar R groups (proline and tryptophan), a charged polar group (arginine) and uncharged polar R groups (serine, citrulline and asparagine) were tested. The snails were more attracted to the uncharged polar R group amino acid serine than to other groups of amino acids. The preferred amino acid concentration was 80 mM. The attraction of snails to different amino acids was concentration dependent. Snails could discriminate amongst the different amino acids at > or = 50 mM.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400016SnailsLymnaea acuminataFasciolaAmino acidsAttraction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tiwari F. Singh D.K. |
spellingShingle |
Tiwari F. Singh D.K. Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species Brazilian Journal of Medical and Biological Research Snails Lymnaea acuminata Fasciola Amino acids Attraction |
author_facet |
Tiwari F. Singh D.K. |
author_sort |
Tiwari F. |
title |
Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species |
title_short |
Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species |
title_full |
Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species |
title_fullStr |
Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species |
title_full_unstemmed |
Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species |
title_sort |
attraction to amino acids by lymnaea acuminata, the snail host of fasciola species |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 0034-7310 |
publishDate |
2004-01-01 |
description |
Adult Lymnaea acuminata (average length 20-22 mm) were collected locally from lakes and low-lying submerged fields from Gorakhpur. The chemoattraction studies were made in round glass aquaria measuring 30 cm in diameter and filled to a depth of 10 mm with 500 ml dechlorinated tap water. Each aquarium was divided into four concentric zones. At the starting time of the assay 10 snails were placed on the circumference of outermost zone 0. Snail attractant pellets (SAP) were added simultaneously in the center of central zone 3. SAP of different amino acids were prepared at concentrations of 10, 20, 50, 80 and 100 mM/2% agar solution and, subsequently, spread to a uniform thickness of 5 mm. After cooling, SAP were cut in small pieces of 5 mm in diameter. Lymnaea acuminata's attraction to amino acids was studied using different amino acid concentrations in SAP. Pellets containing amino acids with non-polar R groups (proline and tryptophan), a charged polar group (arginine) and uncharged polar R groups (serine, citrulline and asparagine) were tested. The snails were more attracted to the uncharged polar R group amino acid serine than to other groups of amino acids. The preferred amino acid concentration was 80 mM. The attraction of snails to different amino acids was concentration dependent. Snails could discriminate amongst the different amino acids at > or = 50 mM. |
topic |
Snails Lymnaea acuminata Fasciola Amino acids Attraction |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400016 |
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