THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN

THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN

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The aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY) diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer using electroejaculator. Immediately after collection the semen was evaluated macroscopic and microscopically. After initial...

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Main Authors: W. M. M. Nalley, R. Handarini, T. L. Yusuf, B. Purwantara, G. Semiadi
Format: Article
Language:English
Published: Diponegoro University, Faculty of Animal and Agricultural Sciences 2011-06-01
Series:Journal of the Indonesian Tropical Animal Agriculture
Subjects:
glucose
glycerol
Timor deer semen
Tris
Online Access:http://www.jppt.undip.ac.id/pdf/36(2)2011p91-96.pdf
id doaj-337406ee629d4bb5892096006b66ffc9
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spelling doaj-337406ee629d4bb5892096006b66ffc92020-11-25T01:30:59ZengDiponegoro University, Faculty of Animal and Agricultural SciencesJournal of the Indonesian Tropical Animal Agriculture2087-82732011-06-013629196THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMENW. M. M. NalleyR. HandariniT. L. YusufB. PurwantaraG. SemiadiThe aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY) diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer using electroejaculator. Immediately after collection the semen was evaluated macroscopic and microscopically. After initial evaluation, the semen was divided into three tubes and extended with Tris egg yolk with three different glycerol concentrations, which were 10% (TGEY10); 12% (TGEY12) and 14% (TGEY14). The sperm motility, viability, acrosome intact and membrane intact were evaluated in raw semen, after equilibration and after thawing. The results showed that there were no differences (p>0.05) on the sperm motility, viability as well as sperm acrosome intact. Sperm membrane intact in TGEY10 (52.50±5.89%) and TGEY14 (51.50±4.12 %) were higher (p<0.05) than in TGEY12 (49.00±6.58). It was concluded that 10, 12 or 14% glycerol concentration can be used for Timor deer semen cryopreservation.http://www.jppt.undip.ac.id/pdf/36(2)2011p91-96.pdfglucoseglycerolTimor deer semenTris
collection DOAJ
language English
format Article
sources DOAJ
author W. M. M. Nalley
R. Handarini
T. L. Yusuf
B. Purwantara
G. Semiadi
spellingShingle W. M. M. Nalley
R. Handarini
T. L. Yusuf
B. Purwantara
G. Semiadi
THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN
Journal of the Indonesian Tropical Animal Agriculture
glucose
glycerol
Timor deer semen
Tris
author_facet W. M. M. Nalley
R. Handarini
T. L. Yusuf
B. Purwantara
G. Semiadi
author_sort W. M. M. Nalley
title THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN
title_short THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN
title_full THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN
title_fullStr THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN
title_full_unstemmed THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN
title_sort effect of glycerol concentration in tris glucose egg yolk extender on the quality of timor deer frozen semen
publisher Diponegoro University, Faculty of Animal and Agricultural Sciences
series Journal of the Indonesian Tropical Animal Agriculture
issn 2087-8273
publishDate 2011-06-01
description The aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY) diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer using electroejaculator. Immediately after collection the semen was evaluated macroscopic and microscopically. After initial evaluation, the semen was divided into three tubes and extended with Tris egg yolk with three different glycerol concentrations, which were 10% (TGEY10); 12% (TGEY12) and 14% (TGEY14). The sperm motility, viability, acrosome intact and membrane intact were evaluated in raw semen, after equilibration and after thawing. The results showed that there were no differences (p>0.05) on the sperm motility, viability as well as sperm acrosome intact. Sperm membrane intact in TGEY10 (52.50±5.89%) and TGEY14 (51.50±4.12 %) were higher (p<0.05) than in TGEY12 (49.00±6.58). It was concluded that 10, 12 or 14% glycerol concentration can be used for Timor deer semen cryopreservation.
topic glucose
glycerol
Timor deer semen
Tris
url http://www.jppt.undip.ac.id/pdf/36(2)2011p91-96.pdf
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