Cyclodextrine Screening for the Chiral Separation of Amlodipine Enantiomers by Capillary Electrophoresis

Purpose: Amlodipine is a long acting, dihydropyridine type calcium channel blocker frequently used in the treatment of hypertension and coronary insufficiency. The calcium channel blocking activity resides primarily in the S-amlodipine enantiomer, while R-amlodipine is a potent inhibitor of smooth m...

Full description

Bibliographic Details
Main Authors: Gabriel Hancu, Monica Budău, Lajos Kristóf Kántor, Anca Cârje
Format: Article
Language:English
Published: Tabriz University of Medical Sciences 2015-03-01
Series:Advanced Pharmaceutical Bulletin
Subjects:
Online Access:http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol5-2015/5-Hancu.pdf
id doaj-3379d7253a414e75ae171f82bf6683e2
record_format Article
spelling doaj-3379d7253a414e75ae171f82bf6683e22020-11-25T00:48:02ZengTabriz University of Medical Sciences Advanced Pharmaceutical Bulletin2228-58812251-73082015-03-0151354010.5681/apb.2015.005Cyclodextrine Screening for the Chiral Separation of Amlodipine Enantiomers by Capillary ElectrophoresisGabriel Hancu0Monica Budău1Lajos Kristóf Kántor2Anca Cârje3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Tîrgu Mureş, Romania.Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Tîrgu Mureş, Romania.Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Tîrgu Mureş, Romania.Department of Analytical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Tîrgu Mureş, Romania.Purpose: Amlodipine is a long acting, dihydropyridine type calcium channel blocker frequently used in the treatment of hypertension and coronary insufficiency. The calcium channel blocking activity resides primarily in the S-amlodipine enantiomer, while R-amlodipine is a potent inhibitor of smooth muscle cell migration. Methods: In this study capillary electrophoresis was applied for the enantiomeric separation of amlodipine using different native and derivatized; neutral and charged cyclodextrines as chiral selectors. The effects of pH and composition of the background electrolyte, concentration and type of chiral selector, capillary temperature, running voltage and injection parameters have been investigated. Results: Stereoselective interactions were observed when using α-CD, β-CD, HP-β-CD, RAMEB, CM-β-CD and SBE-β-CD. Optimized separation conditions consisted on a 50 mM phosphate buffer, pH – 3.0, 20 mM RAMEB as chiral selector, + 25 kV applied voltage, 15°C temperature and UV detection at 238 nm. Using the optimized electrophoretic conditions we succeeded the chiral separation of amlodipine enantiomers in approximately 6 minute, the order of migration being R-amlodipine followed by S-amlodipine. The method was successfully applied for the determination of amlodipine enantiomers from commercially available pharmaceuticals. The linearity range, limits of detection and quantification, precision and accuracy were determined and the results obtained confirmed that the method was suitable for this purpose. Conclusion: It can be concluded that the proposed capillary electrophoresis methods can be useful for routine pharmaceutical applications with benefits of its effectivity, simplicity, short analysis time and low consumption of analytes, solvents and chiral selectors.http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol5-2015/5-Hancu.pdfAmlodipineCapillary electrophoresisChiral SeparationCyclodextrines
collection DOAJ
language English
format Article
sources DOAJ
author Gabriel Hancu
Monica Budău
Lajos Kristóf Kántor
Anca Cârje
spellingShingle Gabriel Hancu
Monica Budău
Lajos Kristóf Kántor
Anca Cârje
Cyclodextrine Screening for the Chiral Separation of Amlodipine Enantiomers by Capillary Electrophoresis
Advanced Pharmaceutical Bulletin
Amlodipine
Capillary electrophoresis
Chiral Separation
Cyclodextrines
author_facet Gabriel Hancu
Monica Budău
Lajos Kristóf Kántor
Anca Cârje
author_sort Gabriel Hancu
title Cyclodextrine Screening for the Chiral Separation of Amlodipine Enantiomers by Capillary Electrophoresis
title_short Cyclodextrine Screening for the Chiral Separation of Amlodipine Enantiomers by Capillary Electrophoresis
title_full Cyclodextrine Screening for the Chiral Separation of Amlodipine Enantiomers by Capillary Electrophoresis
title_fullStr Cyclodextrine Screening for the Chiral Separation of Amlodipine Enantiomers by Capillary Electrophoresis
title_full_unstemmed Cyclodextrine Screening for the Chiral Separation of Amlodipine Enantiomers by Capillary Electrophoresis
title_sort cyclodextrine screening for the chiral separation of amlodipine enantiomers by capillary electrophoresis
publisher Tabriz University of Medical Sciences
series Advanced Pharmaceutical Bulletin
issn 2228-5881
2251-7308
publishDate 2015-03-01
description Purpose: Amlodipine is a long acting, dihydropyridine type calcium channel blocker frequently used in the treatment of hypertension and coronary insufficiency. The calcium channel blocking activity resides primarily in the S-amlodipine enantiomer, while R-amlodipine is a potent inhibitor of smooth muscle cell migration. Methods: In this study capillary electrophoresis was applied for the enantiomeric separation of amlodipine using different native and derivatized; neutral and charged cyclodextrines as chiral selectors. The effects of pH and composition of the background electrolyte, concentration and type of chiral selector, capillary temperature, running voltage and injection parameters have been investigated. Results: Stereoselective interactions were observed when using α-CD, β-CD, HP-β-CD, RAMEB, CM-β-CD and SBE-β-CD. Optimized separation conditions consisted on a 50 mM phosphate buffer, pH – 3.0, 20 mM RAMEB as chiral selector, + 25 kV applied voltage, 15°C temperature and UV detection at 238 nm. Using the optimized electrophoretic conditions we succeeded the chiral separation of amlodipine enantiomers in approximately 6 minute, the order of migration being R-amlodipine followed by S-amlodipine. The method was successfully applied for the determination of amlodipine enantiomers from commercially available pharmaceuticals. The linearity range, limits of detection and quantification, precision and accuracy were determined and the results obtained confirmed that the method was suitable for this purpose. Conclusion: It can be concluded that the proposed capillary electrophoresis methods can be useful for routine pharmaceutical applications with benefits of its effectivity, simplicity, short analysis time and low consumption of analytes, solvents and chiral selectors.
topic Amlodipine
Capillary electrophoresis
Chiral Separation
Cyclodextrines
url http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol5-2015/5-Hancu.pdf
work_keys_str_mv AT gabrielhancu cyclodextrinescreeningforthechiralseparationofamlodipineenantiomersbycapillaryelectrophoresis
AT monicabudau cyclodextrinescreeningforthechiralseparationofamlodipineenantiomersbycapillaryelectrophoresis
AT lajoskristofkantor cyclodextrinescreeningforthechiralseparationofamlodipineenantiomersbycapillaryelectrophoresis
AT ancacarje cyclodextrinescreeningforthechiralseparationofamlodipineenantiomersbycapillaryelectrophoresis
_version_ 1725257177186172928