Evaluation of deoxynivalenol production in dsRNA Carrying and Cured Fusarium graminearum isolates by AYT1 expressing transformed tobacco
Introduction: Fusarium head blight (FHB), is the most destructive disease of wheat, producing the mycotoxin deoxynivalenol, a protein synthesis inhibitor, which is harmful to humans and livestock. dsRNAmycoviruses-infected-isolates of Fusariumgraminearum, showed changes in morphological and pathogen...
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University of Isfahan
2015-12-01
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doaj-34f00cd0252c49a5acfd3afa8bbef2792020-11-24T22:22:15ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812015-12-014155568Evaluation of deoxynivalenol production in dsRNA Carrying and Cured Fusarium graminearum isolates by AYT1 expressing transformed tobaccoMohammad Hasan shahhosseiny0Maryam Ghahri1Mohammad Amin Mahmoudi2Elham Moslemi3 Islamic Azad University, Shahr-e-Qods Branch, Department of Microbiology, Tehran/Iran Iranian Gene Fanavar Institute (IGF) Tehran/Iran Iranian Gene Fanavar Institute (IGF) Tehran/Iran Islamic Azad University, East Tehran Branch, Biology department, Tehran/Iran. Introduction: Fusarium head blight (FHB), is the most destructive disease of wheat, producing the mycotoxin deoxynivalenol, a protein synthesis inhibitor, which is harmful to humans and livestock. dsRNAmycoviruses-infected-isolates of Fusariumgraminearum, showed changes in morphological and pathogenicity phenotypes including reduced virulence towards wheat and decreased production of trichothecene mycotoxin (deoxynivalenol: DON). Materials and methods: Previous studies indicated that over expression of yeast acetyl transferase gene (ScAYT1) encoding a 3-O trichothecene acetyl transferase that converts deoxynivalenol to a less toxic acetylated form, leads to suppression of the deoxynivalenol sensitivity in pdr5 yeast mutants. To identify whether ScAYT1 over-expression in transgenic tobacco plants can deal with mycotoxin (deoxynivalenol) in fungal extract and studying the effect of dsRNA contamination on detoxification and resistance level, we have treated T1 AYT1 transgenic tobacco seedlings with complete extraction of normal F. graminearum isolate carrying dsRNA metabolites. First, we introduced AYT1into the model tobacco plants through Agrobacterium-mediated transformation in an attempt to detoxify deoxynivalenol. Results: In vitro tests with extraction of dsRNA carrying and cured isolates of F. graminearum and 10 ppm of deoxynivalenol indicated variable resistance levels in transgenic plants. Discussion and conclusion: The results of this study indicate that the transgene expression AYT1 and Fusarium infection to dsRNA can induce tolerance to deoxynivalenol, followed by increased resistance to Fusarium head blight disease of wheat.http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-444-1&slc_lang=en&sid=1Fusarium head blight Detoxification Deoxynivalenol ScAYT1 dsRNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammad Hasan shahhosseiny Maryam Ghahri Mohammad Amin Mahmoudi Elham Moslemi |
spellingShingle |
Mohammad Hasan shahhosseiny Maryam Ghahri Mohammad Amin Mahmoudi Elham Moslemi Evaluation of deoxynivalenol production in dsRNA Carrying and Cured Fusarium graminearum isolates by AYT1 expressing transformed tobacco Biological Journal of Microorganism Fusarium head blight Detoxification Deoxynivalenol ScAYT1 dsRNA |
author_facet |
Mohammad Hasan shahhosseiny Maryam Ghahri Mohammad Amin Mahmoudi Elham Moslemi |
author_sort |
Mohammad Hasan shahhosseiny |
title |
Evaluation of deoxynivalenol production in dsRNA Carrying and Cured Fusarium graminearum isolates by AYT1 expressing transformed tobacco |
title_short |
Evaluation of deoxynivalenol production in dsRNA Carrying and Cured Fusarium graminearum isolates by AYT1 expressing transformed tobacco |
title_full |
Evaluation of deoxynivalenol production in dsRNA Carrying and Cured Fusarium graminearum isolates by AYT1 expressing transformed tobacco |
title_fullStr |
Evaluation of deoxynivalenol production in dsRNA Carrying and Cured Fusarium graminearum isolates by AYT1 expressing transformed tobacco |
title_full_unstemmed |
Evaluation of deoxynivalenol production in dsRNA Carrying and Cured Fusarium graminearum isolates by AYT1 expressing transformed tobacco |
title_sort |
evaluation of deoxynivalenol production in dsrna carrying and cured fusarium graminearum isolates by ayt1 expressing transformed tobacco |
publisher |
University of Isfahan |
series |
Biological Journal of Microorganism |
issn |
2322-5173 2322-5181 |
publishDate |
2015-12-01 |
description |
Introduction: Fusarium head blight (FHB), is the most destructive disease of wheat, producing the mycotoxin deoxynivalenol, a protein synthesis inhibitor, which is harmful to humans and livestock. dsRNAmycoviruses-infected-isolates of Fusariumgraminearum, showed changes in morphological and pathogenicity phenotypes including reduced virulence towards wheat and decreased production of trichothecene mycotoxin (deoxynivalenol: DON).
Materials and methods: Previous studies indicated that over expression of yeast acetyl transferase gene (ScAYT1) encoding a 3-O trichothecene acetyl transferase that converts deoxynivalenol to a less toxic acetylated form, leads to suppression of the deoxynivalenol sensitivity in pdr5 yeast mutants. To identify whether ScAYT1 over-expression in transgenic tobacco plants can deal with mycotoxin (deoxynivalenol) in fungal extract and studying the effect of dsRNA contamination on detoxification and resistance level, we have treated T1 AYT1 transgenic tobacco seedlings with complete extraction of normal F. graminearum isolate carrying dsRNA metabolites. First, we introduced AYT1into the model tobacco plants through Agrobacterium-mediated transformation in an attempt to detoxify deoxynivalenol.
Results: In vitro tests with extraction of dsRNA carrying and cured isolates of F. graminearum and 10 ppm of deoxynivalenol indicated variable resistance levels in transgenic plants.
Discussion and conclusion: The results of this study indicate that the transgene expression AYT1 and Fusarium infection to dsRNA can induce tolerance to deoxynivalenol, followed by increased resistance to Fusarium head blight disease of wheat. |
topic |
Fusarium head blight Detoxification Deoxynivalenol ScAYT1 dsRNA |
url |
http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-444-1&slc_lang=en&sid=1 |
work_keys_str_mv |
AT mohammadhasanshahhosseiny evaluationofdeoxynivalenolproductionindsrnacarryingandcuredfusariumgraminearumisolatesbyayt1expressingtransformedtobacco AT maryamghahri evaluationofdeoxynivalenolproductionindsrnacarryingandcuredfusariumgraminearumisolatesbyayt1expressingtransformedtobacco AT mohammadaminmahmoudi evaluationofdeoxynivalenolproductionindsrnacarryingandcuredfusariumgraminearumisolatesbyayt1expressingtransformedtobacco AT elhammoslemi evaluationofdeoxynivalenolproductionindsrnacarryingandcuredfusariumgraminearumisolatesbyayt1expressingtransformedtobacco |
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