The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles

Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in samples is crucial. Lipids removal from HM pr...

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Bibliographic Details
Main Authors: Anna Ojo-Okunola, Shantelle Claassen-Weitz, Kilaza S. Mwaikono, Sugnet Gardner-Lubbe, Heather J. Zar, Mark P. Nicol, Elloise du Toit
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/3/2/39