Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian Children
Aim: The purpose of this study was to investigate the presence of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus species in an American Indian population displaying a high incidence of severe early childhood caries (S-ECC) and to explore the genotypic diversity and fidelity of trans...
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doaj-35147c1e135e4af994a8c3e1a0281a542021-10-04T04:38:03ZengFrontiers Media S.A.Frontiers in Dental Medicine2673-49152021-10-01210.3389/fdmed.2021.740900740900Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian ChildrenAlissa Villhauer0David Lynch1Taylor Postler2Taylor Postler3Deborah Dawson4David Drake5Iowa Institute for Oral Health Research, University of Iowa College of Dentistry, Iowa City, IA, United StatesIowa Institute for Oral Health Research, University of Iowa College of Dentistry, Iowa City, IA, United StatesIowa Institute for Oral Health Research, University of Iowa College of Dentistry, Iowa City, IA, United StatesPrimary Health Care, Ames, IA, United StatesIowa Institute for Oral Health Research, University of Iowa College of Dentistry, Iowa City, IA, United StatesIowa Institute for Oral Health Research, University of Iowa College of Dentistry, Iowa City, IA, United StatesAim: The purpose of this study was to investigate the presence of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus species in an American Indian population displaying a high incidence of severe early childhood caries (S-ECC) and to explore the genotypic diversity and fidelity of transmission of S. sobrinus in this population.Methods: We report here on the microbial profiles of 71 children compiled from birth to 36 months of age and initial exploration of genotypic diversity in a subset of 40 mother/child dyads. Whole mouth plaque samples were collected from mother/child dyads in a Northern Plains American Indian cohort. Mutans streptococci (MS) and Lactobacillus counts were recorded and presence/absence of S. mutans, S. sobrinus, and Lactobacillus was noted for each child. S. sobrinus was isolated and genotyped via arbitrarily primed-polymerase chain reaction (AP-PCR).Results: The children in this study were divided into caries active (CA) and caries free (CF) groups. S. mutans, S. sobrinus, and Lactobacillus species were detected more frequently in CA groups. Distribution of MS and Lactobacillus counts was significantly different between the two groups, as was the distribution of three species colonization profiles. Twelve S. sobrinus genotypes were detected in the subset of mother/child dyads. Individual participants displayed a range of 0–3 distinct genotypes while mother/child pairs displayed a range of 1–5 genotypes. Eighty-three percent of children shared at least one genotype with his/her mother.Conclusion: Caries active children in this population display different microbial profiles than the caries free children in this cohort. A small number of S. sobrinus genotypes appear to be very common in this population. A high rate of vertical transmission of S. sobrinus from mother to child is seen in these mother/child dyads.https://www.frontiersin.org/articles/10.3389/fdmed.2021.740900/fullStreptococcus sobrinusStreptococcus mutanslactobacillisevere early childhood cariesoral health disparitiesgenotyping |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alissa Villhauer David Lynch Taylor Postler Taylor Postler Deborah Dawson David Drake |
spellingShingle |
Alissa Villhauer David Lynch Taylor Postler Taylor Postler Deborah Dawson David Drake Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian Children Frontiers in Dental Medicine Streptococcus sobrinus Streptococcus mutans lactobacilli severe early childhood caries oral health disparities genotyping |
author_facet |
Alissa Villhauer David Lynch Taylor Postler Taylor Postler Deborah Dawson David Drake |
author_sort |
Alissa Villhauer |
title |
Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian Children |
title_short |
Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian Children |
title_full |
Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian Children |
title_fullStr |
Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian Children |
title_full_unstemmed |
Mutans Streptococci and Lactobacilli: Colonization Patterns and Genotypic Characterization of Cariogenic Bacterial Species in American Indian Children |
title_sort |
mutans streptococci and lactobacilli: colonization patterns and genotypic characterization of cariogenic bacterial species in american indian children |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Dental Medicine |
issn |
2673-4915 |
publishDate |
2021-10-01 |
description |
Aim: The purpose of this study was to investigate the presence of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus species in an American Indian population displaying a high incidence of severe early childhood caries (S-ECC) and to explore the genotypic diversity and fidelity of transmission of S. sobrinus in this population.Methods: We report here on the microbial profiles of 71 children compiled from birth to 36 months of age and initial exploration of genotypic diversity in a subset of 40 mother/child dyads. Whole mouth plaque samples were collected from mother/child dyads in a Northern Plains American Indian cohort. Mutans streptococci (MS) and Lactobacillus counts were recorded and presence/absence of S. mutans, S. sobrinus, and Lactobacillus was noted for each child. S. sobrinus was isolated and genotyped via arbitrarily primed-polymerase chain reaction (AP-PCR).Results: The children in this study were divided into caries active (CA) and caries free (CF) groups. S. mutans, S. sobrinus, and Lactobacillus species were detected more frequently in CA groups. Distribution of MS and Lactobacillus counts was significantly different between the two groups, as was the distribution of three species colonization profiles. Twelve S. sobrinus genotypes were detected in the subset of mother/child dyads. Individual participants displayed a range of 0–3 distinct genotypes while mother/child pairs displayed a range of 1–5 genotypes. Eighty-three percent of children shared at least one genotype with his/her mother.Conclusion: Caries active children in this population display different microbial profiles than the caries free children in this cohort. A small number of S. sobrinus genotypes appear to be very common in this population. A high rate of vertical transmission of S. sobrinus from mother to child is seen in these mother/child dyads. |
topic |
Streptococcus sobrinus Streptococcus mutans lactobacilli severe early childhood caries oral health disparities genotyping |
url |
https://www.frontiersin.org/articles/10.3389/fdmed.2021.740900/full |
work_keys_str_mv |
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